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Antioxidative, Antimutagenic, and Cytotoxic Activities of Ethanol Extracts from Cornus officianalis

산수유(Cornus officianalis) 에탄올 추출물의 항산화, 항돌연변이 활성 및 암세포 성장 억제 효과

  • Jeon, Yeon-Hee (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Mi-Hyun (Dept. of Food Science and Nutrition, Kyungpook National University) ;
  • Kim, Mee-Ra (Dept. of Food Science and Nutrition, Center for Beautiful Aging, Kyungpook National University)
  • 전연희 (경북대학교 식품영양학과) ;
  • 김미현 (경북대학교 식품영양학과) ;
  • 김미라 (경북대학교 식품영양학과, 장수생활과학연구소)
  • Published : 2008.01.31

Abstract

The antioxidative, antimutagenic and cytotoxic activities of ethanol extract from Cornus officianalis have been studied. The antioxidant activity of the ethanol extract was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The inhibition effects on the mutagenicity in Salmonella Typhimurium TA100 were evaluated by Ames test and cancer cell inhibitory effects in Hep3B cell and HeLa cell were tested by MTT assay. Cornus officianalis had an important free radical-scavenging activity towards the DPPH radical. At a concentration of 500 ppm, the DPPH radical-scavenging activity of Cornus officianalis was similar to that of L-ascorbic acid. None of the extracts produced a mutagenic effect on S. Typhimurium TA100. The ethanol extract from Cornus officianalis showed about 77% of inhibition at 500 ppm on the mutagenicity induced by 4-nitroquinoline-1-oxide. The extract from Cornus officianalis showed strong cytotoxicity against Hep3B and HeLa cells, with inhibition of 83 and 78% at a dose of $700{\mu}g$/plate, respectively. Moreover, the ethanol extracts had 34.33 mg H.E/g of polyphenols and 5.67 mg Q.E/g of flavonoids, respectively. Therefore, the present study showed antioxidative, antimutagenic and anticancer potential of the ethanol extract from Cornus officianalis.

본 연구에서는 산수유 에탄올 추출물의 항산화능, 항돌연변이 및 항암활성을 분석하여 새로운 생리활성 물질의 탐색과 고부가가치의 기능성식품으로서의 이용 가능성을 알아 보고자 하였다. 산수유 에탄올 추출물의 전자공여능은 농도 의존적으로 증가하여 500 ppm의 농도에서는 합성항산화제인 BHT보다 높았고 천연항산화제인 L-ascorbic acid와 유사하였다. 뿐만 아니라, S. Typhimurium TA100에 대해 산수유 에탄올 추출물은 직접돌연변이원인 4-NQO 사용 시 항돌연변이 효과가 우수한 것으로 나타났다. 또한, 산수유 에탄올 추출물은 $700{\mu}g/plate$ 첨가하였을 때 인체 간암 세포와 자궁경부암 세포의 증식을 모두 78% 이상 억제하였다. 산수유 에탄올 추출물은 비교적 높은 함량의 폴리페놀과 플라보노이드를 함유하고 있어, 이들 물질이 항산화와 항암효과에 영향을 미칠 것으로 사료되었다. 그러나 산수유에는 비타민 C를 비롯한 다른 항산화 성분도 함유되어 있으므로 이들의 항산화성도 추출물의 항산화력에 영향을 주었을 것으로 생각된다. 결론적으로, 산수유 에탄올 추출물은 항산화능과 항돌연변이 활성이 있었으며 인체 간암세포, 자궁경부암 세포 및 대장암 세포에 대해 항암 활성이 있는 것으로 나타나 기능성식품의 소재로서의 이용 가능성을 보여주었다.

Keywords

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