Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Angiotensin Converting Enzyme Inhibition and Antihypertensive Effects of Phyllostachys pubescens Culm Extracts in Spontaneously Hypertensive Rats

맹종죽 줄기 추출물을 투여한 본태성 고혈압 쥐(SHR)의 ACE 저해 활성 및 혈압 강하 효과

  • Kim, Jung-Suk (School of Food and Life Science, and Biohealth Products Research Center and Food Science Institute, Inje University) ;
  • Kim, Mi-Jeong (BK21 Smart Food and Drug Center, Inje University) ;
  • Park, Min-Hee (School of Food and Life Science, and Biohealth Products Research Center and Food Science Institute, Inje University) ;
  • Ryu, Bog-Mi (Dept. of Food Science Institute, Inje University) ;
  • Moon, Gap-Soon (School of Food and Life Science, and Biohealth Products Research Center and Food Science Institute, Inje University)
  • 김정숙 (인제대학교 식품생명과학과, 바이오헬스소재연구센터(BPRC) 및 식품과학연구소) ;
  • 김미정 (인제대학교 BK21 식의약생명공학사업단) ;
  • 박민희 (인제대학교 식품생명과학과, 바이오헬스소재연구센터(BPRC) 및 식품과학연구소) ;
  • 류복미 (인제대학교 식품과학연구소) ;
  • 문갑순 (인제대학교 식품생명과학과, 바이오헬스소재연구센터(BPRC) 및 식품과학연구소)
  • Published : 2008.01.31
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Abstract

This study investigated the anti-hypertensive effect of Phyllostachys pubescens culm extract (PCE) by examining its effects on renal angiotensin-converting enzyme (ACE) inhibition and blood pressure using the spontaneously hypertensive rat (SHR) system. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured weekly for 8 weeks. Also, total antioxidant capacity and protein oxidation of tissues were examined by plasma Trolox Equivalent Antioxidant Capacity assay (TEAC) and hepatic protein carbonyl values, respectively. Twenty male SHR were randomly divided into four groups: PCE50, PCE100, and PCE500 (50, 100, and 500 mg of PCE per kilogram bodyweight daily, respectively), and control group. At week 2, the SBP in all PCE groups appeared to be significantly lower than the control (p<0.05), whereas the DBP were not different until week 4 (p<0.05). At week 8, SBP in the PCE500 was lower by 20% than the control. PCE groups considerably suppressed ACE dose-dependently compared with the control. Plasma TEAC and hepatic protein carbonyl values indicated increased antioxidative activity due to the PCE feed. No adverse effect was observed on the liver of SHR as there was no difference for the GOT and GPT values among the groups. Results of this study suggest that ACE inhibition may be one possible mechanism for the blood pressure lowering effect of PCE; thus, long term consumption of PCE may be beneficial in preventing high blood pressure along with the increased antioxidative status.

사람의 본태성 고혈압 연구 모델인 SHR에 PCE 에탄올 추출물을 농도별로 음용수로 섞어서 8주간 공급한 결과, SBP 및 DBP 모두 대조군에 비해 유의적으로 감소되었다. 특히, PCE에 의한 SBP 저하효과는 2주째부터 현저하였으며 PCE 농도에 의존적이었고 DBP는 4주째에 유의적인 차이가 나타나기 시작하여 이후의 실험시간 동안 낮게 유지되었다. 신장 조직에서 측정한 ACE 저해활성은 고농도의 PCE군들(PCE100 및 PCE500)에서 대조군보다 유의적으로 높게 나타났다. 혈장의 총항산화능은 PCE500에서 매우 높았으며 모든 PCE군들이 대조군에 비해 유의적으로 높았다. 조직의 단백질 산화는 PCE 투여에 의해 절반 정도로 억제되며 간 손상의 지표로 사용한 GOT 및 GPT는 PCE 투여군과 대조군 사이에 차이가 나타나지 않았다. 이상의 실험결과로 미루어볼 때, 줄기부의 활용도가 높은 맹종죽 줄기 추출물은 본태성 고혈압 예방의 목적으로 상용할 수 있는 천연식물 자원으로서의 가치가 높을 것으로 짐작되며, ACE 활성저해는 조직 산화의 억제 및 총항산화력의 증가와 더불어 PCE에 의한 혈압 저하의 기전으로 설명될 수 있을 것으로 사료된다. 끝으로, 선행 연구 및 본 연구에서 드러난 맹종죽 줄기 추출물의 in vitro 및 in vivo ACE 저해활성으로 볼 때, 맹종죽 줄기에서 ACE 활성저해의 원인 물질을 분리 동정해 내는 것이 향후 연구의 귀결점이 되어야할 것으로 판단된다.

Keywords

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