Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Characterization of Microorganisms in Eoyukjang

어육장의 미생물학적 특성

  • Oh, Eu-Jin (Department of Food Science and Technology, Ewha Womans University) ;
  • Oh, Mi-Hwa (Department of Food Science and Technology, Ewha Womans University) ;
  • Lee, Jong-Mee (Department of Food and Nutritional Sciences, Ewha Womans University) ;
  • Cho, Mi-Sook (Department of Food and Nutritional Sciences, Ewha Womans University) ;
  • Oh, Sang-Suk (Department of Food Science and Technology, Ewha Womans University)
  • 오유진 (이화여자대학교 식품공학과) ;
  • 오미화 (이화여자대학교 식품공학과) ;
  • 이종미 (이화여자대학교 식품영양학과) ;
  • 조미숙 (이화여자대학교 식품영양학과) ;
  • 오상석 (이화여자대학교 식품공학과)
  • Published : 2008.12.31
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Abstract

Fermented soybean foods are an important component of the Korean diet. Eoyukjang is a type of traditional fermented soybean source. Microbial analysis of eoyukjang was conducted during the fermentation period in this study. Microorganisms isolated from eoyukjang were identified by biochemical tests and 16S rDNA sequencing. 17 different microorganisms, including bacteria, yeast, and fungi were detected in eoyukjang during the fermentation period. Even though Aspergillus participated in the early stage of fermentation of eoyukjang, Bacillus species and Saccharomyces cerevisiae were the major microzymes in eoyukjang throughout the maturation period. Eoyukjang is generally consumed after the boiling of the final sample. Therefore, the final sample of eoyukjang was boiled and analyzed. Our results showed that no vegetative microorganisms survived under the boiling conditions for eoyukjang. Fermented soybean products in the domestic market were also assessed for comparison with the results from eoyukjang. The total cell number of kanjang (soy sauce) samples was between 0 to 42 CFU/mL. The isolated microorganisms were identified as Bacillus species. All Bacillus isolates were not found to harbor the three enterotoxin-producing and emetic toxin-producing genes.

예로부터 전통 장류는 먹거리로 많이 이용되어 왔으며, 최근에는 장류의 다양한 영양 성분 및 생리 활성 능력이 밝혀지면서 관심이 증대되고 있다. 본 연구에서는 전통 방식으로 제조된 어육장의 발효에 관여하는 미생물을 숙성 기간별로 분석하고 이를 통해 안전성을 평가하였으며, 이와 함께 국내에서 시판되고 있는 간장의 미생물을 분석하여 어육장의 미생물분석과 비교하였다. 어육장은 2개월 단위로 샘플링하여 분석하였으며, 분리된 미생물은 API kit와 16S rDNA sequencing을 이용하여 동정하였다. 어육장의 발효에 전반적으로 관여하는 미생물은 Bacillus 균류와 Saccharomyces cerevisiae였으며, 숙성 초기에는 Aspergillus flavus가 발효에 관여하는 것으로 확인되었다. 어육장은 최종적으로 섭취 전에 달이는 과정을 거치게 되는데 실험결과 달인 후에는 미생물이 존재하지 않았다. 현재 국내에서 시판되고 간장의 경우 총 균수가 0-42 CFU/mL였고, 동정결과 B. subtilis, B. licheniformis, B. pumilus로 확인되었다. 분리된 Bacillus 균류에 대하여 독소여부를 분석한 결과 어육장 및 시판 장류에서 검출된 Bacillus균류는 본 실험에서 분석된 3가지 설사형 독소와 1종의 구토형 독소를 생성하지 않는 균주들인 것으로 확인되었다.

Keywords

References

  1. Jang JH. Fermentation Characteristics and functional effects of Chungkuk-jang using Bacillus spp. isolated during fermentation. PhD Thesis, Korea University. Seoul, Korea (2004)
  2. Cheighm HS, Lee JS, Lee CY. Antioxidative characteristics of melanoidin related products fractionated from fermented soybean sauce. J. Korean Soc. Food Sci. Nutr. 22: 570-575 (1993)
  3. Jung KO, Park SY, Park KY. Longer aging time increase the anticancer and antimetastic properties of Doenjang. J. Nutr. 22: 539-545 (2006) https://doi.org/10.1016/j.nut.2005.11.007
  4. Yoon MK, Choi A, Cho IH, You MJ, Kim JW, Cho MS, Lee JM, Kim YS. Characterization of volatile component in Eoyukjang. Korean J. Food Sci. Technol. 39: 366-371 (2007)
  5. Kim DH, Yook HS, Park BJ, Byun MW. Enhancement of preservation characteristics of Meju, an intermediate material for Koran legume-based fermented soy sauce, Kanjang, by irradiation. Int. J. Radiat. Phys. Ch. 64: 317-322 (2002) https://doi.org/10.1016/S0969-806X(01)00674-0
  6. Han GH, Cho TY, Yoo MS, Kim CS, Kim JM, Kim HA, Kim MO, Kim SC, Lee SA, Ko YS, Kim SH, Kim DB. Biogenic amines formation and content in fermented soybean paste (Cheonggukjang). Korean J. Food Sci. Technol. 39: 541-545 (2007)
  7. Lund BM. Food-borne disease due to Bacillus and Clostridium species. Lancet 336: 982-986 (1990) https://doi.org/10.1016/0140-6736(90)92431-G
  8. Kim YS, Ahn YS, Jeong DY, Shin DH. Isolation and identification of Bacillus cereus from fermented red pepper-soybean paste (Kochujang), and its heat resistance characteristics. Food Sci. Biotechnol. 17: 123-129 (2008)
  9. Garbutt J. Essential of food microbiology. Hodder Arnold. London, Great Britain (1997)
  10. Juneja VK. Control of foodborne microorganisms. Marcel Dekker. New York, NY, USA (2002)
  11. KFDA: Notification No. 2008-6. Korea Food & Drug Administration. Seoul, Korea (2008)
  12. Burgess G, Horwood P. Development of improved molecular detection methods for Bacillus Cereus Toxins. RIRDC, Australian Government, Australia (2006)
  13. From C, Pukall R, Schumann P, Hormazabal V, Granum PE. Toxin-producing ability among Bacillus spp. outside the Bacillus cereus group. J. Appl. Environ. Microbiol. 71: 1178-1183 (2005) https://doi.org/10.1128/AEM.71.3.1178-1183.2005
  14. Byun MO, Lee SB, Koo MS, Song JK, Ryu JC, Lee DH. Identification and characterization of osmotolerant yeast isolated from soy paste. Korean J. Microbiol. 27: 181-186 (1999)
  15. Chin HS, Breidt F, Fleming HP, Shin WC, Yoon SS. Identification of predominant bacterial isolates from the fermenting Kimchi using ITS-PCR and partial 16S rDNA sequence analyses. J. Microbiol. Biotechn. 16: 68-76 (2006)
  16. Lee WJ, Cho DH. Microbiological studies of Korean native soysauce fermentation. J. Korean Agric. Chem. Soc. 14: 137-148 (1971)
  17. Yoo SK, Cho WH, Kang SM, Lee SH. Isolation and identification of microorganisms in korean traditional soybean paste and soybean sauce. Korean J. Microbiol. 27: 113-117 (1999)
  18. Lee TS, Chu YH, Shin BK, Yu JH. Studies on the preservation of soy source. Korean J. Food Sci. Technol. 7: 200-207 (1975)
  19. Mantynen V, Lindstrom K. A rapid PCR-based DNA test for enterotoxic Bacillus cereus. J. Appl. Microbiol. 64: 1634-1639 (1998)
  20. Agata N, Gominet M, Okstad OA. The nceT gene of Bacillus cereus encodes an enterotoxic protein. J. Microbiol. 141: 983-988 (1995) https://doi.org/10.1099/13500872-141-4-983
  21. Lund T, De Buyser ML, Granum PE. A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. Mol. Microbiol. 38: 254-261 (2000) https://doi.org/10.1046/j.1365-2958.2000.02147.x