Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Cytoprotective Effect by Antioxidant Activity of Codonopsis lanceolata and Platycodon grandiflorum Ethyl Acetate Fraction in Human HepG2 Cells

인간 HepG2 세포에서 더덕 및 도라지 에틸아세테이트 분획물의 항산화 효과에 의한 세포보호 효과

  • Kim, Soo-Hyun (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University) ;
  • Choi, Hyun-Jin (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University) ;
  • Oh, Hyun-Taek (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University) ;
  • Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University) ;
  • Cui, Cheng-Bi (Department of Food Science and Engineering, Agricultural College of Yanbian University) ;
  • Ham, Seung-Shi (Department of Food Science and Biotechnology, School of Biotechnology, Kangwon National University)
  • 김수현 (강원대학교 식품생명공학) ;
  • 최현진 (강원대학교 식품생명공학) ;
  • 오현택 (강원대학교 식품생명공학) ;
  • 정미자 (강원대학교 BK21 사업단(뉴트라슈티컬바이오)) ;
  • 최승필 (연변대학농학원 식품과학학부) ;
  • 함승시 (강원대학교 식품생명공학)
  • Published : 2008.12.31
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Abstract

The objective of this study was to determine whether Codonopsis lanceolata or Platycodon grandiflorum ethyl acetate fraction (CLEA or PGEA) protect cells against sodium nitroprusside (SNP)-induced oxidative stress via the expression of various antioxidant systems. The HepG2 cells exposed for 24 hr to 0.5 mM SNP showed a reduction in the cell viability by an MTT assay. Pretreatment with CLEA and PGEA resulted in an inhibition of SNP-induced cell death. In addition, the effects of CLEA and PGEA on the expression of antioxidant systems via RT-PCR analyses was assessed. The levels of catalase (CAT), glucose-6-phosphate dehydrogenase (G6PD) and metallothionein (MT)-1A mRNA were increased after 24 hr of CLEA exposure. The levels of Mn superoxide dismutase CAT, G6PD, MT-1A, and MT-2A mRNA were increased after PGEA treatment. In conclusion, CLEA and PGEA exert indirect antioxidant effects, perhaps via the induction of a variety of antioxidant systems which, may protect cells against oxidative stress.

이 연구의 목적은 더덕 및 도라지 에틸아세테이트 분획물(CLEA나 PGEA)이 다양한 항산화 시스템을 가동시켜 sodium nitroprusside(SNP)에 의해 유도된 산화적 스트레스에 대항하여 세포를 보호해 줄 수 있을지 연구하는 것이다. 0.5 mM의 SNP를 HepG2 세포주에 24시간 동안 노출시켰을 때 세포 생존율이 감소하였으나, CLEA와 PGEA 천처리에 의해 SNP 노출에 의한 세포 생존율 감소가 저해되었다. 또한 항산화 시스템들 발현에 미치는 CLEA와 PGEA의 영향을 RT-PCR로 알아보았다. Catalase, glucose-6-phosphate dehydrogenase(G6PD) 그리고 metallothionein (MT)-1A mRNA 수준이 CLEA를 세포에 24시간 동안 처리한 후 증가하였고, Mn superoxide dismutase, catalase, G6PD, MT-1A 와 MT-2A mRNA 수준이 PGEA 처리에 의해 증가하였다. 우리는 CLEA와 PGEA가 아마 다양한 항산화 시스템들을 증가시키는 간접적인 항산화 효과를 나타내며, 이들 효과들이 산화적 스트레스로부터 세포를 보호해 줄 것이라 추정하였다.

Keywords

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