Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Inhibitory Effects of Punica granatum L. Extracts on Degranulation in Human Basophilic KU812F Cells

석류 추출물에 의한 인간호염구(KU812F 세포)의 탈과립 억제효과

  • Park, Kyong-Tae (Department of Culinary Arts & Nutrition, Kaya University) ;
  • Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University) ;
  • Chun, Soon-Sil (Department of Food and Nutrition, Sunchon National University)
  • 박경태 (가야대학교 호텔조리영양학과) ;
  • 심선엽 (부경대학교 해양생명과학연구소) ;
  • 전순실 (순천대학교 식품영양학과)
  • Published : 2008.12.31
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Abstract

Punica granatum (PG) evidences a variety of physiological properties, including anti-diabetic, anti-cancer, antiinflammatory, anti-microbial, and anti-oxidative activities. Using the human basophilic KU812F cells, the inhibitory effects of the methanolic extract of PG seed, shell, and juice on calcium ionophore, A23187-induced degranulation were assessed. All of the PG extracts inhibited A23187-induced intracellular $Ca^{2+}$ levels, ${\beta}$-hexosaminidase, and histamine release in a dose-dependent manner. These results showed that all of the PG extracts are potent inhibitors of degranulation in allergic reactions, via the suppression of $Ca^{2+}$ influx.

석류는 항당뇨, 항암, 항산화, 항미생물 및 항염증 효과와 같은 다양한 생리적 특성을 가지고 있다. 석류의 씨, 껍질 및 즙의 메탄올 추출물의 항알레르기 활성을 조사하기 위해, 인간 호염구인 KU812F 세포에 있어, A23187로 유도된 탈과립에 있어 저해효과를 검토하였다. 세포를 각각의 추출물로 처리하여, A23187로 자극한 후, 세포 내 칼슘농도, ${\beta}$-hexosaminidase 및 히스타민 함량을 조사하였다. 각각의 석류 추출물은 A23187에 의해 유도된 세포 내 칼슘 농도, 히스타민 및 ${\beta}$-hexosaminidase 유리를 농도 의존적으로 감소시켰다. 이러한 결과는 석류 추출물은 알레르기 반응에 있어 칼슘 유입의 억제를 통한 탈과립의 강력한 저해제로서의 가능성을 제시하고 있다.

Keywords

References

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