Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Separation and Identification of Antimicrobial Substances from Prunus mume extract

매실추출물로부터 항균물질의 분리 및 구조동정

  • Park, Woo-Po (Division of Food Science, Masan College) ;
  • Lee, Seung-Cheol (Division of Food and Biotechnology, Kyungnam University) ;
  • Kim, Sung-Yong (Department of Agricultural Economic, Gyeongsang National University) ;
  • Choi, Sung-Gil (Department of Food Science and Technology, and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Heo, Ho-Jin (Department of Food Science and Technology, and Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Cho, Sung-Hwan (Department of Food Science and Technology, and Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 박우포 (마산대학 식품과학부) ;
  • 이승철 (경남대학교 식품생명학과) ;
  • 김성용 (경상대학교 농업경제학과) ;
  • 최성길 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 허호진 (경상대학교 식품공학과.농업생명과학연구원) ;
  • 조성환 (경상대학교 식품공학과.농업생명과학연구원)
  • Published : 2008.12.31
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Abstract

Prunus mume was extracted using various solvents including ethyl ether, ethyl acetate and n-butanol. The extracts were fractionated by column chromatography. Antimicrobial compounds (A, B and C) in fractions showing antimicrobial activity were purified by Sephadex LH 20 column chromatography, and identified by $^{1}H$- and $^{13}C$-NMR analysis as isoeugenol, nomilin and $\beta$-sitosterol, respectively.

항균력이 탁월한 매실추출물로부터 항균활성물질을 column chromatography를 이용하여 순수하게 분리하고, nuclear magnetic resonance (NMR) spectrophtometer 측정기에 의하여 항균활성물질의 화학구조를 다음과 같이 분리 동정하였다. 즉, 매실추출물을 상온에서 메탄올에 현탁한 후, ethyl ether, ethyl acetate, n-butanol로 각각 분배 추출하여 각 용매의 수용성 추출물을 획득하였다. 각 용매의 수용성 추출물을 loading한 후, silica gel(70-230 mesh, Merck) column chromatography하여 소획분을 얻었다. 분리된 분획 중 항균력이 있는 소획분을 Sephadex LH 20을 충진하고 column chromatography하여 각 항균분획물질을 분리하였다. column chromatography에서 분리한 각 항균분획물질(Compound A, Compound B, Compound C)의 구조분석을 위해 $^{1}H$ 및 $^{13}C$ NMR 스펙트럼을 실온에서 측정하였다. NMR은 Bruker DRX500 (500MHz)을 이용하여 측정하였으며, 용매와 기준물질은 $CDCl_3$와 TMS를 각각 사용하였다. 이와같이 분리한 각 항균분획물질을 NMR 분석법으로 동정한 결과, isoeugenol, nomilin 및 $\beta$-sitosterol 등으로 확인되었다.

Keywords

References

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