Effect of Aster scaber Juice supplemented with Carrot and Ginseng extracts on Serum Lipid in Rats

인삼과 당근 즙액 첨가 참취녹즙이 흰쥐의 혈청지질에 미치는 영향

  • Choi, Ae-Ran (Department of Hotel & Food Service Industry, Joongbu University) ;
  • Seung, Suk-Kyung (Department of Dining Atmosphere Display, The Graduate School of Tourism & Hospitality)
  • 최애란 (중부대학교 호텔외식산업학과) ;
  • 성숙경 (경기대학교 관광전문대학원 식공간연출학과)
  • Published : 2008.12.31

Abstract

The effect on serum lipid of green aster juice blended with ginseng and carrot extracts was investigated using rats injected with $CCl_4$. The rats were classified with four groups: (i) normal control (NC), (ii) rats fed with the blended juice (BA), (iii) rats treated with $CCl_4$ after having been on a normal diet for 4 weeks ($NC-CCl_4$), and (iv) rats treated with $CCl_4$ after having been fed the blended juice for 4 weeks ($BA-CCl_4$). All groups had similar feed intake. The weight gains and feed efficiency ratio were lower in the $NC-CCl_4$ group. The liver weight per body weight was much higher in the $NC-CCl_4$ group than the NC group, but did not differ between the $BA-CCl_4$ and BA groups. Triglycerides increased only for the $NC-CCl_4$ group (88.72 mg/dL); the other groups had similar levels (56.48-65.33 mg/dL). The BA group had the lowest total cholesterol level (74.08 mg/dL) the other groups had similar levels (96.78-108.83 mg/dL). HDL-cholesterol was lower in the $NC-CCl_4$ group (40.56 mg/dL) compared with the NC group (48.95 mg/dL), but there was no difference between the BA and $BA-CCl_4$ groups. The LDL-cholesterol level was higher in the $NC-CCl_4$ group (55.20 mg/dL the highest level) than the NC group (43.33 mg/dL), and higher in the $BA-CCl_4$ group (50.10 mg/dL) than the BA group (18.09 mg/dL). The lipid peroxide content was much higher in the $NC-CCl_4$ group (22.61 nmol/g) than the NC group (12.52 nmol/g), but the $BA-CCl_4$ (17.41 nmol/g) and BA (13.99 nmol/g) groups were similar. The glutathione content was much lower in the $NC-CCl_4$ group ($2.25\;{\mu}mol/g$) than the NC and BA groups, and decreased to $2.63\;{\mu}mol/g$ for the $BA-CCl_4$ group. The glutathione content of the $BA-CCl_4$ recovered to the level of that in the NC group.

본 연구에서는 참취녹즙 : 당근즙액 : 인삼즙액의 혼합비율을 66.5 : 28.5 : 5로 혼합하여 제조한 혼합 참취녹즙이 사염화탄소 투여 흰쥐의 혈청지질에 미치는 영향을 조사하였다. 실험군은 정상식이군(NC군), 혼합 참취녹즙 식이군(BA군), 정상식이 4주 후 $CCl_4$를 투여하는 정상식이 사염화탄소 대조군($NC-CCl_4$군) 및 혼합 참취녹즙 식이 4주 후 $CCl_4$를 투여하는 혼합 참취녹즙 사염화탄소 대조군($BA-CCl_4$군)의 4군이었다. 식이섭취량은 모든 처리군 간에 차이가 없었으며, 증체량과 식이효율은 $NC-CCl_4$군만 크게 감소하였다. 체중 당 간의 중량비는 $NC-CCl_4$군은 NC군보다 크게 증가하였으나, $BA-CCl_4$군은 BA군과 유의적 차이가 없었다. 중성지질은 $NC-CCl_4$군만 유의적으로 증가하였고, $BA-CCl_4$군은 NC군이나 BA군과 차이가 없었다. 총 콜레스테롤 함량은 BA군만 낮았다. HDL-콜레스테롤 함량은 $NC-CCl_4$군은 유의적으로 감소하였으나, $BA-CCl_4$군은 NC군이나 BA군과 차이가 없었다. LDL-콜레스테롤 함량은 $NC-CCl_4$군(55.20 mg/dL)은 NC군(43.33 mg/dL)에 비해 유의적으로 증가하였으며, $BA-CCl_4$군(50.10 mg/dL)은 BA군(18.09 mg/dL)보다는 증가하였으나 $NC-CCl_4$군보다는 감소하는 경향이었다. Lipid peroxide 함량은 $NC-CCl_4$군은 NC군보다 크게 증가하였으나, $BA-CCl_4$군은 BA군과 유의적인 차이를 보이지 않았으며 $NC-CCl_4$군에 비해서는 크게 감소하였다. Glutathione 함량은 $NC-CCl_4$군이 $2.25\;{\mu}moles/g$로서 NC군과 BA군에 비해 크게 감소하였다. 그러나 $BA-CCl_4$군은 $2.63\;{\mu}moles/g$로서 BA군보다는 감소하였으나 NC군 수준으로 회복되었다.

Keywords

References

  1. Han, J.S., Kim, J.S., Kim, M.S., Choi, Y.H. and Huh, S.M. (1999) Changes on mineral contents of vegetagles by various cooking methods. Korean J. Soc. Food Sci., 15, 382-387
  2. Yoo, K.O., Ahn, S.D., Chun, I.J., Hong, J.K., Yoo, C.Y., Kim, J.H., Kim, S.C. and Lim, H.T. (1996) Comparative studies of the five edible mountain vegetables by DNA marker fingerprinting. Korean J. Plant Res., 9, 305-310
  3. Lee, J.M. and Chung, H.J. (1999) Physicochemical and Sensory Characteristics of snack using cham chwi(Aster scaber). Korean J. Dietary Culture, 14, 49-55
  4. Park, J.A. and Kim, M.K. (1999) Effect of Korean native plant diet on lipid metabolism, antioxidative capacity and cadmium deoxification in rats. Korean J. Nutr., 32, 353-357
  5. Kim, S.J., Kim, C.K. and Kim, G.H. (2004) Quality characteristics of aster scaber and development of functional healthy drinks using its extract. Korean J. Soc. Food Cookery Sci., 20, 310-316
  6. Lee, H.J., Han, D.S. and Kim, M.K. (2001) Effect of dried powder and juice of aster scaber on lipid metabolism and antioxidative capacity in rats. Korean J. Nutr., 34, 375-383
  7. Pachla, L.A., Reyholds, D.L. and Kissing, P.T. (1985) Analytical methods for determining ascorbic acid in biological samples, food products, and pharmaceuticals. J. Assoc. Off. Anal. Chem., 68, p.2-5
  8. Rose, R.C. and Nagrwold, D.L. (1981) Quantitative analysis of ascorbic acid and dehydroascorbic acid by high-performance liquid chromatography. Anal. Biochem., 114, p.140-145 https://doi.org/10.1016/0003-2697(81)90464-4
  9. Choi, A.R., Lee, M.Y., Sung, S.K. and Chang, K.H. (2007) Quality characteristics of Aster scaber green juice prepared by addition of ginseng and carrot juices. Nat. Sci. Inst., 16, 67-75
  10. LEE, S.M., Yu, R., Rhee, S.H. and Park, K.Y. (1997) Effects of carrot on the stability of vitamin C in (green-yellow) vegetable juices. J. Korean Soc. Food Sci. Nutr., 26, p.582-587
  11. Satho, K. (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin. Chim. Acta., 90, 37-43 https://doi.org/10.1016/0009-8981(78)90081-5
  12. Ellman, G.L. (1959) Tissue sulfydryl group. Korean Soc. Food Nutr., 82, 70-77
  13. Jung, M.E., Ham, S.S., Nam, S.M., Kang, I.J., Kim, S.J. and Chung, C.K. (2001) Biochemical and histological effects of phellinus linteus methanol extract on liver lipid metabolism of rats fed $CCL_4$ and high fat. J. Korean Soc. Food Sci. Nutr. 30, 331-337
  14. Ha, T.Y., Cho, I.J. and Lee, H.Y. (2001) Effect of cassia tora ethanol extracts on carbon tetrachlorideind uced liver injury in rats. Korean J. Food Sci. Technol., 33, 789-794
  15. Kim, J.H. and Kim, M.K. (1999) Effect of dried powders and ethanol extracts of Perilla frutescens, Artemisia princeps var. orientalis and Aster scaber on lipid metabolism and antioxidative capacity in rats. Korean J. Nutr. Soc., 32, 540-551
  16. Kim, O.K. (2002) Protective effects of houttuynia cordata thunb on carbon tetrachloride induced hepatotoxicity in rats. Kor. J. Pharmacogn., 33, 324-331
  17. Mun, S.I. (2000) Effect of zanthoxylum schinifolium its active principle on serum lipid levels in carbon tetrachloride-treated mice. Korean J. Food Nutr., 13, 249-254
  18. Lee, J.H., Chi, S.C., Kim, S.H., Shin, Y.H., Park, H.J. and Choi, J.W. (2005) Preventive effect of a schizandrin C derivative DDB-mixed preparation (DWP-04) against hepatotoxicity induced by carbon tetrachloride. Korea J. Pharmacogn., 36, 44-49
  19. Kwon, S.M., Yoon, S., Kim, S.Y., Park, K.S., Yeoh, I.H. and Lee-Kim, Y.C. (2000) Study on lipid lowering effect of green vegetable juice. Ann. Meeting, Symposium of Korean Nutr. Soc., Oct. Abst., p.84
  20. Kim, J.S., Kim, H.Y., Park, Y.K., Kim, T.S. and Kang, M.H. (2003) The effects of green vegetable juice (Angelica keiskei) supplementation on plasma lipids and antioxidant status in smokers. Korean J. Nutr., 36, 933-941
  21. Recknagel, R.O., Glende, E.A. and Hruszkewyez, A.M. (1977) Chemical mechanisms in carbon tetrachloride toxicity. In "Free radicals in biology" Pryor W.A.(ed.), p.97-99
  22. Kim, O.K. (2001) Protective effects of extracts of hovenia dulcis thunb on hepatotoxicity in carbon tetrachloride intoxicated rats. J. Korean Soc. Food Sci. Nutr., 30, 1260-1265
  23. Kim, A.R. and Chung, H.Y. (1995) Role of glutathione against cytotoxicity of t-butyl hydroperoxide in cultured liver cell. Kor. J. Gerontol., 5, 53-58
  24. Husain, S.R., Cillard, J. and Cillard, P. (1987) Hydroxy radical scavenging activity of flavonoids. Phytochemistry, 26, 2489-2491 https://doi.org/10.1016/S0031-9422(00)83860-1
  25. Shulz, H. (1994) Regulation of fatty acid oxidation in heart. J. Nutr., 124, 165-171 https://doi.org/10.1093/jn/124.2.165
  26. Parke, D.V. (1993) The importance of diet and nutrition in the detoxication of chemicals in Food Nutrition and Chemical Toxicity. Smith-Gordon, London, England, p.1-15
  27. Meister, A. and Anderson, M.E. (1983) Glutathion. Ann. Rev. Biochem., 52, 711-760 https://doi.org/10.1146/annurev.bi.52.070183.003431