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유자과피 열수 추출물의 항산화 활성

Antioxidant Activity of Hot-Water Extract from Yuza (Citrus junos SIEB ex TANAKA) Peel

  • 신정혜 (경남도립남해대학 호텔조리제빵과) ;
  • 이수정 (경상대학교 식품영양학과.농업생명과학연구원) ;
  • 서종권 (한국국제대학교 식품과학부) ;
  • 전은우 (한국국제대학교 식품과학부) ;
  • 성낙주 (경상대학교 식품영양학과.농업생명과학연구원)
  • Shin, Jung-Hye (Dept. of Hotel Curinary Arts & Bakery, Gyeongnam Provincial Namhae College) ;
  • Lee, Soo-Jung (Department of Food Science and Nutrition, Institute of Agriculture and Life Scineces, Gyeongsang National University) ;
  • Seo, Jong-Kwon (Devision of Food Science, International University of Korea) ;
  • Cheon, Eun-Woo (Devision of Food Science, International University of Korea) ;
  • Sung, Nak-Ju (Department of Food Science and Nutrition, Institute of Agriculture and Life Scineces, Gyeongsang National University)
  • 발행 : 2008.12.30

초록

산지별 유자의 특성 및 기능성 분석을 위하여 거제, 고성, 고흥 및 남해산(창선 및 설천) 유자를 시료로 하여 과피 열수 추출물을 제조한 다음 다양한 반응계에서 항산화 활성을 비교 분석하였다. 열수추출물 중의 총 페놀 및 플라보노이드 함량은 남해 설천산이 각각 $122.18{\pm}1.44$ mg/100 g과 $114.39{\pm}0.94$ mg/100 g으로 여타 시료에 비하여 유의적으로 높은 함량이었으며 여타 시료간에의 함량 차이는 미미하였다. Hesperidin과 naringin의 함량은 거제산 유자 추출물에서 각각 $55.45{\pm}1.36$ mg/100 g과 $28.41{\pm}0.64$ mg/100 g으로 가장 높은 함량이었다. 유자 열수 추출물의 항산화력은 시료의 첨가 농도가 $500{\sim}10,000{\mu}g$/ml로 증가함에 따라 유의적으로 활성이 증가하였다. 환원력의 경우 10,000 ${\mu}g$/ml농도에서는 500 ${\mu}g$/ml 농도에 비하여 6${\sim}$9배 정도 환원력이 증가하여 흡광도 값은 $0.68{\pm}0.012{\sim}0.97{\pm}0.021$의 범위였다. ABTs 소거 활성은 10,000 ${\mu}g$/ml농도에서 고성산($78.13{\pm}1.30%$)을 제외한 모든 시료에서 80% 이상의 활성을 나타내었다. Hydroxyl radical 소거활성은 10,000 ${\mu}g$/ml 농도에서 남해 설천산 ($31.36{\pm}1.36%$) 및 남해 창선산($30.28{\pm}1.60%$)을 제외한 시료에서 활성은 30% 미만으로 낮았다. 10,000 ${\mu}g$/ml 농도에서 NO 라디칼 소거활성은 $26.49{\pm}1.77{\sim}40.85{\pm}0.95%$의 범위였으며, ${\beta}$-carotene 존재 하에서 항산화능은 $24.40{\pm}1.91{\sim}38.17{\pm}0.56%$로 거제산과 고성산을 제외한 모든 시료에서 30% 이상의 활성을 나타내었다.

This study was to investigate of characteristics and antioxidant function of yuza from 4 different area. The hot-water extracts of yuza peel extracts from Geoje, Goseong, Goheung and Namhae(Changseon, Seolcheon) were tested for antioxidant activity in different reaction systems. Contents of total phenolic compounds and flavonoids were $122.18{\pm}1.44$ mg/100 g and $114.39{\pm}0.94$ mg/100 g in hot water extracts from Namhae-Seolcheon, respectively. The highest contents of hesperidin and naringin were $55.45{\pm}1.36$ mg/100 g and $28.41{\pm}0.64$ mg/100 g in hot water extracts from Geoje, respectively. Antioxidant activity of yuza peel hot-water extracts were significantely increased as the increament of sample adding concentration ($500{\sim}10,000{\mu}g$/ml). Reducing power was $6{\sim}9$ folds higher in 10,000 ${\mu}g$/ml concentration than 500 ${\mu}g$/ml and it's O.D. value showed $0.68{\pm}0.012{\sim}0.97{\pm}0.021$. ABTs scavenging activity was more than 80% in 10,000 ${\mu}g$/ml concentration samples, except from Goseong ($78.13{\pm}1.30%$). Hydroxyl radical scavenging activity was higher in Namhae-Seolcheon ($31.36{\pm}1.36%$) and Namhae-Changseon ($30.28{\pm}1.60%$) at 10,000 ${\mu}g$/ ml concentration, others activity were below 30%. At 10,000 ${\mu}g$/ml concentration, NO radical scavenging activity and antioxidant activity in ${\beta}$-carotene linoleic acid system were $26.49{\pm}1.77{\sim}40.85{\pm}0.95%$ and $24.40{\pm}1.91{\sim}38.17{\pm}0.56%$, respectively.

키워드

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