Sibjeondaebotang and Yugmijihwangtang's Toxicological Effects on Rat's Fetus

십전대보탕과 육미지황탕이 실험동물의 태자에 미치는 독성학적 연구

  • Han, Sang-Baek (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University) ;
  • Jeon, Seong-Jin (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University) ;
  • Shin, Heon-Tae (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University) ;
  • Park, Hae-Mo (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University) ;
  • Lee, Sun-Dong (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University) ;
  • Park, Chul-Soo (Department. of Preventive Medicine, College of Oriental Medicine, Sangji University)
  • 한상백 (상지대학교 한의과대학 예방의학교실) ;
  • 전성진 (상지대학교 한의과대학 예방의학교실) ;
  • 신헌태 (상지대학교 한의과대학 예방의학교실) ;
  • 박해모 (상지대학교 한의과대학 예방의학교실) ;
  • 이선동 (상지대학교 한의과대학 예방의학교실) ;
  • 박철수 (상지대학교 한의과대학 예방의학교실)
  • Published : 2008.12.31

Abstract

Purpose : To evaluate safety of Sibjeondaebotang and Yugmijihwangtang in rats' fetus Methods : Female Sprague-Dawley rats were orally administered with the Sibjeondaebotang and Yugmijihwangtang at dose of 5mg/kg/day for 20 days. Pregnant rats were sacrificed at 20th day of gestation. Approximately live fetuses in the 20th day of gestation were randomly selected and fixed in 95% ethanol. To observe skeletal malformations, fetuses were stained with alcian blue and alizarin red S. Results : Neonatal body weight and number of fetus of Sibjeondaebotang, Yugmijihwangtang group were increased to those of control group. The fetuses treated with Sibjeondaebotang, Yugmijihwangtang didn't showed external malformation. Vertebral and sternal skeletal variations were observed in Sibjeondaebotang, Yugmijihwangtang administered group, but compared to the control, those skeletal variations were insignificant. There were no significant changes in number of ribs, cervical, thoracic, lumbar, sacral and caudal vertebrae Conclusion : From these results, it can be concluded that Sibjeondaebotang, Yugmijihwangtang shows no toxicity effects on fetus body weight and number of live fetuses. Although skeletal variations were shown in vertebrate and sternum, Sibjeondaebotang, Yugmijihwangtang did not show significant changes in bone malformation.