Abstract
Cholesterol and cholesterol oxide products (COPs) of Gulbi (Pseudosciaena manchurica) processed and stored at different salting times of 5 hours and 5 days were analyzed. The content of cholesterol was $133.4{\pm}5.20\;mg/100\;g$ in the fresh sample. Cholesterol content was decreased during salting and storage; its contents were $130.3{\pm}2.95\;mg/100\;g$ and $87.2{\pm}3.49\;mg/100\;g$ in 5 hours and 5 days salting samples, respectively. The content of 7-ketocholesterol in 5 hours and 5 days salting samples were $75.2{\pm}2.70\;{\mu}g/100\;g$ and $82.4{\pm}3.30\;{\mu}g/100\;g$. The 7-ketocholesterol content of salting sample for 5 hours had no significantly difference for 2 days sun drying, but it was dramatically increased during 5 days sun drying and then increased during storage days. $7{\alpha}-$ and $7{\beta}-hydroxycholesterol$ were not detected in fresh sample, but increased during Gulbi processing and storage. The $7{\alpha}-hydroxycholesterol$ was increased during Gulbi processing and storage and a higher level of $658.1{\pm}6.20\;{\mu}g/100\;g$ was detected in 5 hours salting sample than in 5 days salting sample after 21 days storage. The $7{\beta}-hydroxycholesterol$ also showed similar tendency with $7{\alpha}-hydroxycholesterol$; it was largely increased between 7 and 14 days storage in 5 days salting sample.
굴비 가공 중 식염농도가 콜레스테롤 산화에 미치는 영향을 분석하고자 염장시간을 5시간과 5일로 달리하여 건조 저장한 후 콜레스테롤 및 그 산화물의 함량을 분석하였다. 생 시료의 콜레스테롤 함량은 $133.4{\pm}5.20\;mg/100\;g$으로 정량되었으나 천일염으로 염장 후 콜레스테롤 함량은 5시간 염장하였을 때 $130.3{\pm}2.95\;mg/100\;g$, 5일간 염장한 시료에서 $87.2{\pm}3.49\;mg/100\;g$으로 염장시간에 따른 콜레스테롤의 감소가 현저하였으며 저장기간 동안 콜레스테롤의 함량은 점차 감소하였다. 7-ketocholesterol은 5시간 및 5일간 염장 후 각각 $75.2{\pm}2.70\;{\mu}g/100\;g$과 $82.4{\pm}3.30\;{\mu}g/100\;g$으로 정량되었다. 5시간 염장시료는 건조 2일까지는 7-ketocholesterol의 함량에 유의차가 없었으나 건조 5일에 급격히 증가하였고, 저장기간 동안에도 점차 증가하였다. $7{\alpha}$ 및 $7{\beta}-hydroxycholesterol$은 생 시료에서는 전혀 검출되지 않았으나 가공과정을 거치면서 점차 증가하였는데 $7{\alpha}-hydroxycholesterol$의 함량은 저장 21일 후는 5시간 염장한 시료($658.1{\pm}6.20\;{\mu}g/100\;g$)에서 5일간 염장한 시료($539.6{\pm}6.17\;{\mu}g/100\;g$)보다 $118.5\;{\mu}g/100g$ 더 많이 생성되었다. $7{\beta}-hydroxycholesterol$도 염장 및 저장 기간에 따라 그 함량이 증가하였으며 5일간 염장한 시료에서는 저장 7일과 14일 사이에 증가폭이 가장 컸다.
