Cytogenetic Analysis in Korean Head and Neck Cancer Cell Lines: Comparative Genomic Hybridization(CGH) and Array-CGH

두경부 편평상피세포암 세포주의 염색체 이상 분석: 비교유전체보합법과 Array 비교유전체보합법

  • Shin, You-Ree (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Park, Soo-Yeun (Department of Anatomy, School of Medicine, Ewha Womans University) ;
  • Lee, Dong-Wook (Department of Otolaryngology, College of Medicine, Chungbuk National University) ;
  • Kim, Han-Su (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Go, Young-Min (Deparment of Otolaryngology, Seoul Adventist Hospital) ;
  • Park, Hyun-Joo (Department of Otolaryngology, School of Medicine, Ewha Womans University) ;
  • Choung, Sung-Min (Department of Otolaryngology, School of Medicine, Ewha Womans University)
  • 신유리 (이화여자대학교 의과대학 이비인후과학교실) ;
  • 박수연 (이화여자대학교 의과대학 해부학교실) ;
  • 이동욱 (충북대학교 의과대학 이비인후과학교실) ;
  • 김한수 (이화여자대학교 의과대학 이비인후과학교실) ;
  • 고영민 (서울위생병원 이비인후과) ;
  • 박현주 (이화여자대학교 의과대학 이비인후과학교실) ;
  • 정성민 (이화여자대학교 의과대학 이비인후과학교실)
  • Published : 2008.05.31

Abstract

Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15- p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more detailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.

Keywords

References

  1. Koh Ty, Park KH, Lee SG, et al: Inhibitory effect of p27KIP1 gene transfer on head and neck squamous cell carcinoma cell lines. Head Neck. 2003;25:44-49 https://doi.org/10.1002/hed.10166
  2. Bockmühl U, Wolf G, Schmidt S, et al: Genomic alterations associated with malignancy in head and neck cancer. Head Neck. 1998;20:145-151 https://doi.org/10.1002/(SICI)1097-0347(199803)20:2<145::AID-HED8>3.0.CO;2-2
  3. Van Dyke Dl, Worsham MJ, Genninger MS, et al: Recurrent cytogenetic abnormalities in squamous cell carcinoma of the head and neck region, Genes Chromosome Cancer. 1994;3:192-206
  4. Yin XY, Donovan-Peluso M, Whiteside TL, et al: Gene amplification and gene dosage in cell lines derived from squamous cell carcinoma of the head and neck. Genes Chromosome Cancer. 1991;3:443-454 https://doi.org/10.1002/gcc.2870030606
  5. Agochiya M, Brunton VG, Owens DW, et al: Increased dosage and amplification of the focal adhesion kinase gene in human cancer cells. Oncogene. 1999;18:5646-5653 https://doi.org/10.1038/sj.onc.1202957
  6. Bergamo NA, Rogatto SR, Poli-Frederico RC, et al: Comparative genomic hybridization analysis detects frequent over-representation of DNA sequences at 3q, 7p, and 8q in head and neck carcinomas. Cancer Genet Cytogenet. 2000;119:48-55 https://doi.org/10.1016/S0165-4608(99)00213-7
  7. Huang Q, Yu GP, McCormick SA, et al: Genetic differences detected by comparative genomic hybridization in head and neck squamous cell carcinomas from different tumor sites: Construction of oncogenetic trees for tumor progression. Genes Chromosome Cancer. 2002;34:224-233 https://doi.org/10.1002/gcc.10062
  8. Coe BP, Lee EH, Chi B, et al: Gain of a region on 7p22.3, containing MAD1L1, is the most frequent event in small-cell lung cancer cell lines. Genes Chromosome Cancer. 2005;45:11-19
  9. Schlade-Bartusiak K, Stembalska A, Ramsey D: Significant involvement of chromosome 13q deletions in progression of larynx cancer, detected by comparative genomic hybridization. J Appl Genet. 2005;35:407-413
  10. Tanner MM, Tirkkonen M, Kallioniemi A, holli K, Collins C, Kowbel D, Gray JW, Kallioniemi OP, Isola J: Amplification of chromosmal region 20q13 in invasive breast cancer: prognostic implications. Clin Cancer Res. 1995;1:1456-1461
  11. Carinci F, Lo Muzio L, Piattelli A, et al: Potential markers of tongue tumor progression selected by cDNA microarray. Int J Immunopathol Pharmacol. 2005;18:513-524 https://doi.org/10.1177/039463200501800311
  12. Maestro R, Gasparotto D, Vukosavljevic T, Barzan L, Sulfaro S, Boiocchi M: Three discrete regions of deletion at 3p in head and neck cancers. Cancer Res. 1993;53:5775-5779
  13. Roz L, Wu CL, Porter S, et al: Allelic imbalance on chromosome 3p in oral dysplastic lesions: an early event in oral carcinogenesis. Cancer Res. 1996;56:1228-1231
  14. Gotte K, Hadaczek P, Coy JF, et al: Fhit expression is absent or reduced in a subset of primary head and neck cancer. Anticancer Res. 2000;20:1057-1060
  15. Lin SC, Chang MF, Chung MY, et al: Frequent microsatellite alterations of chromosome locus 4q13.1 in oral squamous cell carcinomas. J Oral Pathol Med. 2005;34:209-213 https://doi.org/10.1111/j.1600-0714.2004.00296.x
  16. Smith D, Zhu Y, McAvoy S, Kuhn R: Common fragile sites, extremely large genes, neural development and cancer. Cancer Lett. 2006;28;232(1):48-57
  17. Takebayashi S, Ogawa T, Jung KY, et al: Identification of new minimally lost regions on 18q in head and neck squamous cell carcinoma. Cancer Res. 2000;60:3397-3403
  18. Pearlstein RP, Benninger MS, Carey TE, et al: Loss of 18q predicts poor survival of patients with squamous cell carcinoma of the head and neck. Genes Chromosome Cancer. 1998;21:333-339 https://doi.org/10.1002/(SICI)1098-2264(199804)21:4<333::AID-GCC7>3.0.CO;2-#
  19. Gollin SM: Chromosomal alterations in squamous cell carcinomas of the head and neck: Window to the biology of disease. Head Neck. 2001;23:238-253 https://doi.org/10.1002/1097-0347(200103)23:3<238::AID-HED1025>3.0.CO;2-H
  20. Toomes C, Jackson A, Maguire K, et al: The presence of multiple regions of homozygous deletion at the CSMD1 locus in oral squamous cell carcinoma question the role of CSMD1 in head and neck carcinogenesis. Genes Chromosomes Cancer. 2003;37:132-140 https://doi.org/10.1002/gcc.10191