Differential Gene Expression in a Red Alga Gracilaria textorii(Suringar) Hariot (Gracilariales, Florideophyceae) between Natural Populations

  • Woo, Seon-Ock (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Ko, Young-Wook (Department of Biological Science, Sungkyunkwan University) ;
  • Oh, Yoon-Sik (Division of Life Science, Gyeongsang National University) ;
  • Kim, Jeong -Ha (Department of Biological Science, Sungkyunkwan University) ;
  • Lee, Taek-Kyun (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute) ;
  • Yum, Seung-Shic (Southern Coastal Environment Research Department, Korea Ocean Research and Development Institute)
  • Published : 2008.09.30

Abstract

The bio-molecules involved in defense mechanisms can be used as efficient biomarkers for physiological changes in organisms caused by both of internal and external stress. Thus, the expression level of genes which encoding such molecules serve as critical 'early warning system' for environmental assessment as well as health diagnosis of biological organisms. In this study, Cytochrome P450, Heat shock protein 90, Ubiquitin and ${\beta}$-actin gene were isolated for the first time from a red alga Gracilaria textorii. The quantitative differential gene expression analyses of three genes, GteCYP1A, GteHsp90 and Gte-UB, were carried out in G. textorii sporophytes collected from two different localities, polluted Sujeong (Masan, Korea) and potentially unpolluted Danggeum (Daemaemuldo Is., Korea). The transcripts of all three tested genes were highly expressed in the Sujeong population. The results suggest: 1) the Sujeong site was more polluted than the Danggeum site; 2) G. textorii could be applicable to marine environment monitoring in coastal regions.

Keywords

References

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