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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Hepatitis B virus X protein enhances NFκB activity through cooperating with VBP1

  • Kim, Sang-Yong (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Jin-Chul (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Jeong-Ki (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Kim, Hye-Jin (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Lee, Hee-Min (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Choi, Mi-Sun (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Maeng, Pil-Jae (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University) ;
  • Ahn, Jeong-Keun (Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University)
  • Received : 2007.11.09
  • Accepted : 2007.11.21
  • Published : 2008.02.29
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Abstract

Hepatitis B virus X protein (HBx) is essential for hepatitis B virus infection and exerts a pleiotropic effect on various cellular machineries. HBx has been also demonstrated as an indirect transcriptional transactivator of various different viral and cellular promoters. In addition, HBx is involved in the development of various liver diseases including hepatocellular carcinoma. However the mechanism of HBx in hepatocellular carcinogenesis remains largely unknown. In this study, to identify possible new cellular proteins interacting with HBx, we carried out yeast two-hybrid assay. We obtained several possible cellular partners including VBP1, a binding factor for VHL tumor suppressor protein. The direct physical interaction between HBx and VBP1 in vitro and in vivo was confirmed by immunoprecipitation assay. In addition, we found that VBP1 facilitates HBx-induced $NF{\kappa}B$ activation and cell proliferation. These results implicate the important role of HBx in the development of hepatocellular carcinoma through its interaction with VBP1.

Keywords

References

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