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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Suppressive effects of Lithospermum erythrorhizon extracts on lipopolysaccharide-induced activation of AP-1 and NF-κB via mitogen-activated protein kinase pathways in mouse macrophage cells

  • Han, Kyu-Yeon (Graduate School of Biotechnology and Institute of Life Science & Resources, Kyung Hee University) ;
  • Kwon, Taek-Hwan (Graduate School of Biotechnology and Institute of Life Science & Resources, Kyung Hee University) ;
  • Lee, Tae-Hoon (Graduate School of Biotechnology and Institute of Life Science & Resources, Kyung Hee University) ;
  • Lee, Sung-Joon (Department of Food and Biotechnology, Korea University) ;
  • Kim, Sung-Hoon (Lab of Angiogenesis and Chemoprevention, Department of Oriental Pathology, College of Oriental Medicine, Kyunghee University) ;
  • Kim, Ji-Young (Graduate School of Biotechnology and Institute of Life Science & Resources, Kyung Hee University)
  • Received : 2007.12.12
  • Accepted : 2008.03.11
  • Published : 2008.04.30
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Abstract

A variety of anti-inflammatory agents have been shown to exert chemopreventive activity via targeting of transcription factors such as NF-${\kappa}B$ and AP-1. Lithospermum erythrorhizon (LE) has long been used in traditional oriental medicine. In this study, we demonstrated the inhibitory effects of LE extracts on lipopolysaccharide (LPS)-stimulated production of inflammatory cytokines. As an underlying mechanism of inhibition, LE extracts reduced LPS-induced transactivation of AP-1 as well as NF-${\kappa}B$ in mouse macrophage cells. Electrophoretic mobility shift assays indicated that LE extracts inhibited the DNA binding activities of AP-1 and NF-${\kappa}B$. In addition, phosphorylation of $I{\kappa}B-{\alpha}$ protein was suppressed by LE extracts. Moreover, LE extracts inhibited c-Jun N-terminal kinase and extracellular signal-regulated signaling pathways. Our results suggest that the anti-inflammatory activity of LE extracts may be mediated by the inhibition of signal transduction pathways that normally lead to the activation of AP-1and NF-${\kappa}B$. These inhibitory effects may be useful for chemoprevention of cancer or other chronic inflammatory diseases.

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References

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