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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses

  • Pulla, Rama Krishna (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Myung-Kyum (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Senthil, Kalai Selvi (Kongunadu Arts and Science College) ;
  • In, Jun-Gyo (Biopia Co., Ltd.) ;
  • Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
  • Received : 2007.10.17
  • Accepted : 2007.12.26
  • Published : 2008.04.30
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Abstract

Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.

Keywords

References

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