Journal of Trauma and Injury

The Korean Society of Traumatology (대한외상학회)
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Effect of Heat Shock Protein 70 on Inducible Nitric Oxide Synthase during Sepsis in Rats

패혈증에서 Heat Shock Protein 70의 과도발현이 Inducible Nitric Oxide Synthase의 발현에 미치는 효과

  • Lee, Yong Keun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonbuk National University) ;
  • Shin, Hyo-Keun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonbuk National University) ;
  • Kwon, Woon Yong (Department of Emergency Medicine, Seoul National University College of Medicine) ;
  • Suh, Gil Joon (Department of Emergency Medicine, Seoul National University College of Medicine) ;
  • Youn, Yeo Kyu (Department of Surgery, Seoul National University College of Medicine)
  • 이용근 (전북대학교 치과대학 구강악안면외과학교실) ;
  • 신효근 (전북대학교 치과대학 구강악안면외과학교실) ;
  • 권운용 (서울대학교 의과대학 응급의학교실) ;
  • 서길준 (서울대학교 의과대학 응급의학교실) ;
  • 윤여규 (서울대학교 의과대학 외과학교실)
  • Received : 2008.05.22
  • Accepted : 2008.06.08
  • Published : 2008.06.30
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Abstract

Purpose: The aim of this study was to evaluate the effect of overexpression of heat shock protein 70 (HSP70) on the expression of inducible nitric oxide synthase and on the concentration of nitric oxide and to determine the mechanism for the relationship between HSP70 and inducible nitric oxide synthase (iNOS) in sepsis. Methods: Experiments were performed on male Sprague-Dawley rats, and sepsis was induced by using cecal ligation and puncture (CLP). Glutamine (GLN) or saline was administered 1 h after initiation of sepsis. We acquired serum and lung tissues from the rats 12 h or 24 h after initiation of sepsis. We analyzed the concentration of nitric oxide, the expression of HSP70 in the lung, and the gene expression of iNOS in the lung. Results: In CLP+GLN, glutamine given after initiation of sepsis enhanced the expression of HSP70 in the lung at 12 h (CLP+GLN vs. CLP:: $47.19{\pm}10.04$ vs. $33.22{\pm}8.28$, p = 0.025) and 24 h (CLP+GLN vs. CLP: $47.06{\pm}10.60$ vs. $31.90{\pm}4.83$, p = 0.004). In CLP+GLN, glutamine attenuated the expression of iNOS mRNA in the lung at 12 h (CLP+GLN vs. CLP: $4167.17{\pm}951.59$ vs. $5513.73{\pm}1051.60$, p = 0.025) and 24 h (CLP+GLN vs. CLP: $9,437.65{\pm}2,521.07$ vs. $18,740.27{\pm}8,241.20$, p = 0.016) and reduced the concentration of nitric oxide in serum at 12 h (CLP+GLN vs. CLP: $0.86{\pm}0.48$ vs. $3.82{\pm}2.53{\mu}mol/L$, p = 0.016) and 24 h (CLP+GLN vs. CLP: $0.39{\pm}0.25$ vs. $1.85{\pm}1.70{\mu}mol/L$, p = 0.025). Conclusion: The overexpression of HSP70 induced by the administration of glutamine in sepsis attenuated the gene expression of iNOS and reduced the concentration of nitric oxide.

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