Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Isolation of Aeromonas sobria from Cultured Mud Loach, Misgurnus mizolepis

양식 미꾸라지(Misgurnus mizolepis)로 부터 Aeromonas sobria 검출

  • Yu, Jin-Ha (Department of Aquatic life Medicine, Kunsan National University) ;
  • Park, Sung-Woo (Department of Aquatic life Medicine, Kunsan National University)
  • 유진하 (군산대학교 수산생명의학과) ;
  • 박성우 (군산대학교 수산생명의학과)
  • Published : 20080400
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Abstract

Mass mortality occurred in mud loaches, Misgurnus mizolepis, cultured in ponds located in Kunsan. External signs of affected fish showed hemorrhage of skin and fins, Internally, pale liver with congestion, enlarged kidney, and spleen and enteritis exhibited. Causative bacteria isolated from liver, spleen, and kidney of the disease fish. In biochemical tests, the isolates were similar with those of the reference strains, A. sobria. The aerolysine gene from the present isolate was amplified PCR with the primer SOBF and SOBB for A. sobria. The isolate was identified as A. sobria on the basis of those tests. In virulence test, the present isolate resulted in the development of clinical signs identical to those in naturally infected fish. The present results conclude that the present isolate is A. sobria and can be a pathogen which causes motile aeromonad septicemia to mud loach.

2006년　5월　전북　군산시에　위치한　미꾸라지　양식장에서　미꾸라지가 대량으로 폐사하였다.　병어는　궤양을　동반한　체표의　출혈반점과　아가미　빈혈　및　울혈　있었고　간의　퇴색과　비장, 신장의　종대　및　장의　출혈이　나타났다. 병어의 간, 비장, 신장에서　그람음성의　단간균이　검출되었고　생화학적　시험측정　결과　분리균은 Aeromonas sobria로　판명되었다. 분리균과 A. sobria가 소유한 aerolysine 유전자　일치　여부를 분석한 결과 같은　유전자를　가지고　있음이　판명되었다. 분리균을　미꾸라지에　농도별로　복강주사　한　결과　자연발생어의 증상과　일치하였다. 그럼으로 A. sobria는 양식　미꾸라지의 새로운 세균성　질병의 원인균이 될 가능성이 높다고 판단된다.

Keywords

References

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