Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
권호 표지
DOI QR코드

DOI QR Code

Avian Somitic Cell Chimeras Using Surrogate Eggshell Technology

  • Mozdziak, Paul E. (Department of Poultry Science) ;
  • Hodgson, Dee (Department of Poultry Science) ;
  • Petitte, James N. (Department of Poultry Science)
  • Received : 2007.09.22
  • Accepted : 2007.12.10
  • Published : 2008.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A classical technique to study somitic cell fate is to employ the cross-transplantation of quail somites into a chick host. The densely stained nucleoli of the quail cells makes it possible to assess the fate of the donor quail cells in the chick host. Classical somite transplantation techniques have been hampered by the necessity of a small opening in the chick eggshell, difficulty in hatching the offspring and interspecies post-hatch graft rejection. With the advent of transgenic chicken technology, it is now possible to use embryos from transgenic chickens expressing reporter genes in somite cross-transplantation techniques to remove any possibility of interspecies graft rejection. This report describes using a surrogate eggshell system in conjunction with transgenic chick:chick somitic cell cross-transplantation to generate viable chimeric embryos and offspring. Greater than 40% of manipulated embryos survive past 10 days of incubation, and ~80% of embryos successfully cultured past 10 days of incubation hatched to produce viable offspring.

Keywords

References

  1. Andacht, T., W. Hu and R. Ivarie. 2004. Rapid and improved method for windowing eggs accessing the stage X chicken embryo. Mol. Reprod. Dev. 69:31-34. https://doi.org/10.1002/mrd.20155
  2. Beresford, B. 1983. Brachial muscles in the chick embryo: the fate of individual somites. J. Embryol. Exp. Morphol. 77:99-116.
  3. Beresford, B., C. Le Lievre and M. P. Rathbone. 1978. Chimaera studies of the origin and formation of the pectoral musculature of the avian embryo. J. Exp. Zool. 205:321-326. https://doi.org/10.1002/jez.1402050217
  4. Borwornpinyo, S., J. Brake, P. E. Mozdziak and J. N. Petitte. 2005. Culture of chicken embryos in surrogate eggshells. Poult. Sci. 84:1477-1482. https://doi.org/10.1093/ps/84.9.1477
  5. Chapman, S. C., A. Lawson, W. C. Macarthur, R. J. Wiese, R. H. Loechel, M. Burgos-Trinidad, J. K. Wakefield, R. Ramabhadran, T. J. Mauch and G. C. Schoenwolf. 2005. Ubiquitous GFP expression in transgenic chickens using a lentiviral vector. Development 132:935-940. https://doi.org/10.1242/dev.01652
  6. Chevallier, A., M. Kieny and A. Mauger. 1977. Limb-somite relationship: origin of the limb musculature. J. Embryol. Exp. Morphol. 41:245-258.
  7. Chevallier, A., M. Kieny and A. Mauger. 1978. Limb-somite relationship: effect of removal of somitic mesoderm on the wing musculature. J. Embryol. Exp. Morphol. 43:263-278.
  8. Christ, B. and B. Brand-Saberi. 2002. Limb muscle development. Int. J. Dev. Biol. 46:905-914.
  9. Fineman, R. M. and G. C. Schoenwolf. 1987. Animal-modeldysmorphogenesis and death in a chicken-embryo model. Am. J. Med. Genet. 27:543-552. https://doi.org/10.1002/ajmg.1320270307
  10. Fineman, R. M., G. C. Schoenwolf, M. Huff and P. L. Davis. 1986. Animal-model-causes of windowing-induced dysmorphogenesis (neural-tube defects and early amnion deficit spectrum) in chicken embryos. Am. J. Med. Genet. 25:489-505. https://doi.org/10.1002/ajmg.1320250311
  11. Fisher, M. and G. C. Schoenwolf. 1983. The use of early chickembryos in experimental embryology and teratologyimprovements in standard procedures. Teratol. 27:65-72. https://doi.org/10.1002/tera.1420270110
  12. Giamario, C., J. N. Petitte and P. E. Mozdziak. 2003. Hatchability of chicken embryos following somite manipulation. Biotechniques 34:1128-1130.
  13. Hamburger, V. and H. L. Hamilton. 1951. A series of normal stages in the development of the chick embryo. J. Morphol. 88:49. https://doi.org/10.1002/jmor.1050880104
  14. Harvey, A. J., G. Speksnijder, L. R. Baugh, J. A. Morris and R. Ivarie. 2002. Consistent production of transgenic chickens using replication-deficient retroviral vectors and highthroughput screening procedures. Poult. Sci. 81:202-212. https://doi.org/10.1093/ps/81.2.202
  15. Huang, R., Q. Zhi, C. P. Ordahl and B. Christ. 1997. The fate of the first avian somite. Anat. Embryol. (Berl) 195:435-449. https://doi.org/10.1007/s004290050063
  16. Kinutani, M., M. Coltey and N. M. Le Douarin. 1986. Postnatal development of a demyelinating disease in avian spinal cord chimeras. Cell 45:307-314. https://doi.org/10.1016/0092-8674(86)90395-8
  17. Kinutani, M. and N. M. Le Douarin. 1985. Avian spinal cord chimeras. I. Hatching ability and posthatching survival in homo- and heterospecific chimeras. Dev. Biol. 111:243-255. https://doi.org/10.1016/0012-1606(85)90449-X
  18. Le Douarin, N. 1973. A biological cell labeling technique and its use in experimental embryology. Dev. Biol. 30:217-222. https://doi.org/10.1016/0012-1606(73)90061-4
  19. Le Douarin, N. M. 1973. A feulgen-positive nucleolus. Exp. Cell Res. 77:459-468 https://doi.org/10.1016/0014-4827(73)90600-9
  20. Lee, H. T., K. S. Chung, D. W. Han, K. Whang, K. S. Roh, B. C. Koo, M. S. Kwon, C. K. Lee, Y. T. Heo, T. Kim and Y. M. Lee. 2004. Germ-line transmission of pseudotyped retroviral vector in chicken. Asian-Aust. J. Anim. Sci. 17:27-32. https://doi.org/10.5713/ajas.2004.27
  21. Lee, C. H. Yan, B. Xiao, P. Trefil, S. Liu, Y. Kim and X. Wu. 2005. Production of transgenic chimeric chickens using blastodermal cells. Asian-Aust. J. Anim. Sci. 18:158-164. https://doi.org/10.5713/ajas.2005.158
  22. Mozdziak, P. E., S. Borwornpinyo, D. W. McCoy and J. N. Petitte. 2003. Development of transgenic chickens expressing bacterial beta-galactosidase. Dev. Dyn. 226:439-445. https://doi.org/10.1002/dvdy.10234
  23. Mozdziak, P. E. and J. N. Petitte. 2004. Status of transgenic chicken models for developmental biology. Dev. Dyn. 229:414-421. https://doi.org/10.1002/dvdy.10461
  24. Mozdziak, P. E., Q. Wu, J. M. Bradford, S. L. Pardue, S. Borwornpinyo, C. Giamario and J. N. Petitte. 2006. Identification of the lacZ insertion site and beta-galactosidase expression in transgenic chickens. Cell Tissue Res. 324:41-53. https://doi.org/10.1007/s00441-005-0060-9
  25. Ordahl, C. P. and B. Christ. 1997. Avian somite transplantation: A review of basic methods. Methods in Cell Biology, Vol. 52. 52:3-27. https://doi.org/10.1016/S0091-679X(08)60371-4
  26. Ordahl, C. P. and N. M. Le Douarin. 1992. Two myogenic lineages within the developing somite. Development 114:339-353.
  27. Perry, M. M. 1988. A complete culture system for the chickembryo. Nature 331:70-72. https://doi.org/10.1038/331070a0
  28. Rowlett, K. and K. Simkiss. 1987. Explanted embryo culture - invitro and inovo techniques for domestic-fowl. Br. Poult. Sci. 28:91-101. https://doi.org/10.1080/00071668708416940
  29. Speksnijder, G. and R. Ivarie. 2000. A modified method of shell windowing for producing somatic or germline chimeras in fertilized chicken eggs. Poult. Sci. 79:1430-1433. https://doi.org/10.1093/ps/79.10.1430

Cited by

  1. culture system vol.59, pp.2, 2018, https://doi.org/10.1080/00071668.2017.1413234
  2. Expression of Cdc25B mRNA in Duodenal Mucosa of Chicken vol.23, pp.4, 2008, https://doi.org/10.5713/ajas.2010.90383
  3. Normal Development and Hatchability of Korean Oge Chickens in White Leghorn Surrogate Eggshells vol.41, pp.2, 2008, https://doi.org/10.5536/kjps.2014.41.2.87