Asian-Australasian Journal of Animal Sciences

  • 제21권4호
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  • Pages.596-602
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  • 2008
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Improvement of Functional Properties of Ovotransferrin by Phosphorylation through Dry-heating in the Presence of Pyrophosphate

  • Hayashi, Yoko (United Graduate School of Agricultural Sciences, Kagoshima University) ;
  • Li, Can-Peng (Department of Food and Pharmacy Engineering, School of Chemistry Science and Technology, Yunnan University) ;
  • Enomoto, Hirofumi (United Graduate School of Agricultural Sciences, Kagoshima University) ;
  • Ibrahim, Hisham R. (Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University) ;
  • Sugimoto, Yasushi (United Graduate School of Agricultural Sciences, Kagoshima University) ;
  • Aoki, Takayoshi (Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University)
  • 투고 : 2007.08.28
  • 심사 : 2007.11.19
  • 발행 : 2008.04.01
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초록

Ovotransferrin (OTf) was phosphorylated by dry-heating in the presence of pyrophosphate at pH 4.0 and $85^{\circ}C$ for 1 and 5 d, and the functional properties of phosphorylated OTf (PP-OTf) were investigated. The phosphorus content of OTf increased to 0.91% as a result of phosphorylation and the electrophoretic mobility of PP-OTf also increased. Although the solubility of dry-heated OTf slightly decreased, the decrease was reduced by phosphorylation. The stability against heat-induced insolubilization of OTf was somewhat improved by phosphorylation, but more than 70% of PP-OTf was insolubilized when it was heated at $70^{\circ}C$ for 10 min at pH 7.0. However, heat-induced insolubilization of PP-OTf was reduced when it was heated in the presence of phosphorylated ovalbumin. This may explain the excellent stability of phosphorylated egg white protein against heat-induced insolubilization which was reported previously. The emulsifying property of OTf was also somewhat improved by phosphorylation. The calcium phosphate-solubilizing ability of PP-OTf was enhanced. Although the degree of phosphorylation of OTf by dry-heating in the presence of pyrophosphate was similar to that of ovalbumin, the improvement of properties of PP-OTf was considerably different from those of phosphorylated ovalbumin.

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참고문헌

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