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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Differentially expressed genes in Penaeus monodon hemocytes following infection with yellow head virus

  • Pongsomboon, Siriporn (Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Tang, Sureerat (Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Boonda, Suleeporn (Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University) ;
  • Aoki, Takashi (Laboratory of Genome Science, Tokyo University of Marine Science and Technology) ;
  • Hirono, Ikuo (Laboratory of Genome Science, Tokyo University of Marine Science and Technology) ;
  • Yasuike, Motoshige (Laboratory of Genome Science, Tokyo University of Marine Science and Technology) ;
  • Tassanakajon, Anchalee (Shrimp Molecular Biology and Genomics Laboratory, Department of Biochemistry, Faculty of Science, Chulalongkorn University)
  • Published : 2008.09.30
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Abstract

A cDNA microarray composed of 2,028 different ESTs from two shrimp species, Penaeus monodon and Masupenaeus japonicus, was employed to identify yellow head virus (YHV)-responsive genes in hemocytes of P. monodon. A total of 105 differentially expressed genes were identified and grouped into five different clusters according to their expression patterns. One of these clusters, which comprised five genes including cathepsin L-like cysteine peptidase, hypothetical proteins and unknown genes, was of particular interest because the transcripts increased rapidly ($\leq$ 0.25 hours) and reached high expression levels in response to YHV injection. Microarray data were validated by realtime RT-PCR analyses of selected differentially expressed transcripts. In addition, comparative analysis of the hemocyte transcription levels of three of these genes between surviving and non-surviving shrimp revealed significantly higher expression levels in surviving shrimp.

Keywords

References

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