Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
권호 표지

Inhibitory effects of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa) on larval migration of Anisakis spp. in vitro

시험관내에서 아니사키스 유충의 운동성에 대한 고련피, 관중, 사군자의 억제효과

  • Kwon, Hee-Nyung (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jee, Cha-Ho (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University)
  • 권희녕 (충북대학교 수의과대학 및 동물의학연구소) ;
  • 지차호 (충북대학교 수의과대학 및 동물의학연구소)
  • Accepted : 2008.10.23
  • Published : 2008.12.30
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Abstract

A high incidence of Anisakiasis has been reported in many countries where people eat frequently raw or undercooked seafood. Anisakis spp. larvae were obtained from the mackerel acquired from a fish market of Cheongju city. They were divided into several groups and placed in culture dishes containing RPMI-1640 (culture media), in the presence or absence of different concentrations of herbal extracts (Meliae ezadarach, Dryopteris crassirhizoma, Quisqualis indica var villosa). The objective of the present study was to investigate the activity of larval migration inhibition in vitro. Meliae ezadarach at the concentrations of 7.5, 15, and 30 mg/ml effectively inhibited the larvae migration in time-dependent manner during experimental period of 0-24 h. Treatment of Meliae ezadarach at the three concentrations completely inhibited the larvae migration in vitro. Dryopteris crassirhizoma at the concentrations of 5, 10, and 20 mg/ml also effectively inhibited the larvae migration in a time-dependent manner. The treatment of Dryopteris crassirhizoma for 12 h completely inhibited the larvae migration. The inhibitory effect of Dryopteris crassirhizoma was stronger than that of Meliae ezadarach. Although Quisqualis indica var villosa also showed the inhibitory effect on larvae migration, its inhibitory efficacy was the weakest among tested herbal extracts. These results indicated that some herbal extracts may be useful in controlling human anisakiasis.

Keywords

References

  1. 김승남. 관중의 항균작용 및 세포독성에 관한 연구. 서울대학교 대학원 석사학위 논문, 2000
  2. 김태훈. 메트로니다졸 내성 질편모충에 대한 고련피의 약제 효과. 경희대학교 대학원 석사학위논문, 2002
  3. 도농선, 민병선, 배기환. 관중의 항균성물질 분리 및 충치균에 대한 항균력 평가. 약학논문집 1996, 12, 111-114
  4. 박원식. 고련피 살충효과에 대한 in vitro실험. 경희대학교 대학원 석사학위논문, 2000
  5. 이재구. 최신수의기생충학. pp. 137-138, 대한교과서, 서울, 1999
  6. 이지영, 백남인, 신동화, 한지숙. 관중(Dryopteris crassirhizoma Nakai)의 식중독 미생물 증식 억제 물질의 분리 및 항균작용. 한국식품과학회지 2001, 33, 611-618
  7. 임경일, 신호준. 환자로부터 분리한 Anisakis (Nematoda: Anisakidae)유충의 형태학적 특징 및 분류학적 고찰. Yonsei Rep Trop Med 1993, 24, 39-46
  8. 임경일, 신호준, 김병화, 문승일. 고래회충유충증 107 례 보고 및 어류감염 실태 조사. 기생충학잡지 1995, 33, 179-186
  9. 전계식. 충무 어시장 붕장어(Astroconger myriaster)의 아니사키스 유충 감염상. 한국환경위생학회지 1997, 23, 14-17
  10. 전계식. 위 아니사키스증에 관한 문헌적 고찰. 자연과학연구소논문지 2000, 5, 33-37
  11. 최경림. 톡소포자충에 대한 고련피의 약제 효과에 따른 미세 구조적 변화. 경희대학교 대학원 박사학위논문, 2003
  12. 한국생약학교수협의회. 본초학. pp. 462-463, 대한약사회, 서울, 1995
  13. 허준. 동의보감. p. 162, 남산당, 서울, 1989
  14. Abollo E, Gestal C, Pascual S. Anisakis infestation in marine fish and cephalopods from galician waters: an updated perspective. Parasitol Res 2001, 87, 492-429 https://doi.org/10.1007/s004360100389
  15. Besprozvannykh VV, Ermolenko AV, Kamnev VD. Peter-the-Great Gulf fishes are a source of human invasion of the nematode Anisakis simplex (the family Anisakidae). Med Parazitol (Mosk) 2004, 3, 21-24
  16. d'Assonville JA, Janovsky E, Verster A. In vitro screening of Haemonchus contortus third stage larvae for ivermectin resistance. Vet Parasitol 1996, 61, 73-80 https://doi.org/10.1016/0304-4017(95)00807-1
  17. Douch PGC, Morum PE. The effects of anthelmintics on ovine larval nematode parasite migration in vitro. Int J Parasitol 1994, 24, 321-326 https://doi.org/10.1016/0020-7519(94)90078-7
  18. Doupe, Lymbery AJ, Wong S, Hobbs RP. Larval anisakid infections of some tropical fish species from north-west Australia. J Helmintol 2003, 77, 363-365 https://doi.org/10.1079/JOH2003193
  19. Iglesias L, Valero A, Bentez R, Adroher FJ. In vitro cultivation of Anisakis simplex: pepsin increases survival and moulting from fourth larval to adult stage. Parasitology 2001, 123, 285-291
  20. Kim CH, Chung BS, Moon YI, Chun SH. A case report on human infection with Anisakis sp. in Korea. Kisaengchunghak Chapchi 1971, 9, 39-43
  21. Koyama T, Kobayashi A, Kumada M, Komiya Y, Oshima T, Kagei N, Ishii T, Machida M. Morphological and taxonomical studies on Anisakidae larvae found in marine fishes and squids. Jpn J Parasitol 1969, 18, 466-487
  22. Lee SM, Na MK, An RB, Min BS, Lee HK. Antioxidant Activity of Two Phloroglucinol Derivatives from Dryopteris crassirhizoma. Bio Pharm Bull 2003, 26, 1354-1356 https://doi.org/10.1248/bpb.26.1354
  23. Lopez-Serrano MC, Gomez AA, Daschner A, Moreno-Ancillo A, de Parga JMs, Caballero MT, Barranco P, Cabañas R. Gastroallergic anisakiasis : findings in 22 patients. J Gastroenterol Hepatol 2000, 15, 503-506 https://doi.org/10.1046/j.1440-1746.2000.02153.x
  24. Manfredi MT, Crosa G, Galli P, Ganduglia S. Distribution of Anisakis simplex in fish caught in the Ligurian Sea. Parasitol Res 2000, 86, 551-553 https://doi.org/10.1007/s004360000202
  25. Noh JH, Kim B, Kim SM, Ock M, Park MI, Goo JY. A case of acute gastric anisakiasis provoking severe clinical problems by multiple infection. Korean J Parasitol 2003, 41, 97-100 https://doi.org/10.3347/kjp.2003.41.2.97
  26. Nuchjangreed C, Hamzah Z, Suntornthiticharoen P, Sorosjinda-Nunthawarasilp P. Anisakids in marine fish from the coast of Chon Buri Province, Thailand. Southeast Asian J Trop Med Public Health 2006, 37 (Suppl), 35-39
  27. Pampiglione S, Rivasi F, Criscuolo M, De Benedittis A, Gentile A, Russo S, Testini M. Human anisakiasis in Italy: a report of eleven new cases. Pathol Res Pract 2002, 198, 429-434 https://doi.org/10.1078/0344-0338-00277
  28. Skírnisson K. Pseudoterranova decipiens (Nematoda, Anisakidae) larvae reported from humans in Iceland after consumption of insufficiently cooked fish. Laeknabladid 2006, 92, 21-25
  29. Valero A, Martin-Sanchez J, Reyes-Muelas E, Adroher FJ. Larval anisakids parasitizing the blue whiting, Micromesistius poutassou, from Motril Bay in the Mediterranean region of southern Spain. J Helminthol 2000, 74, 361-364 https://doi.org/10.1017/S0022149X00000536
  30. van Thiel PH, Kuipers FC, Roskam RT. A nematode parasitic to herring, causing acute abdominal syndromes in man. Trop Geogr Med 1960, 12, 97-113
  31. Vieno NM, Tuhkanen T, Kronberg L. Analysis of neutral and basic pharmaceuticals in sewage treatment plants and in recipient rivers using solid phase extraction and liquid chromatography-tandem mass spectrometry detection. J Chromatogr A 2006, 17, 101-111
  32. Wagland BM, Jones WO, Hribar L, Bendixsen T, Emery DL. A new simplified assay for larval migration inhibition. Int J Parasitol 1992, 22, 1183-1185 https://doi.org/10.1016/0020-7519(92)90040-R