BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Transgenic tobacco plants overexpressing the Nicta; CycD3; 4 gene demonstrate accelerated growth rates

  • Guo, Jia (School of Biotechnology, Kangwon National University) ;
  • Wang, Myeong-Hyeon (School of Biotechnology, Kangwon National University)
  • Published : 2008.07.31
Download PDF
⟨ Previous Next ⟩

Abstract

D-type cyclins control the onset of cell division and the response to extracellular signals during the G1 phase. In this study, we transformed a D-type cyclin gene, Nicta;CycD3;4, from Nicotiana tabacum using an Agrobacterium-mediated method. A predicted 1.1 kb cyclin gene was present in all of the transgenic plants, but not in wild-type. Northern analyses showed that the expression level of the Nicta;CycD3;4 gene in all of the transgenic plants was strong when compared to the wild-type plants, suggesting that Nicta;CycD3;4 gene driven by the CaMV 35S promoter was being overexpressed. Our results revealed that transgenic plants overexpressing Nicta;CycD3;4 had an accelerated growth rate when compared to wild-type plants, and that the transgenic plants exhibited a smaller cell size and a decreased cell population in young leaves when compared to wild-type plants.

Keywords

References

  1. Potuschak, T. and Doerner, P. (2001) Cell cycle controls: genome-wild analysis in Arabidopsis. Curr. Opin. Plant. Biol. 4, 501-506. https://doi.org/10.1016/S1369-5266(00)00207-7
  2. Umeda, M., Bhalerao, R., Schell, J., Uchimiya, H. and Koncz, C. (1998) A distinct cyclin-dependent kinase-activating kinase of Arabidopsis thaliana. Proc. Natl. Aca. Sci. U.S.A. 95, 5021-5026. https://doi.org/10.1073/pnas.95.9.5021
  3. Renaudin, J. P., Doonan, J. H., Freeman, D., Hashimoto, J., Hirt, H., Inzé, D., Jacobs, T., Kouchi, H., Rouzé, P., Sauter, M., Savouré, A., Sorrell, D. A., Sundaresan, V. and Murray, J. A. H. (1996) Plant cyclins: a unified nomenclature for plant A-, B- and D-type cyclins based on sequence organization. Plant Mol. Biol. 32, 1003-1018. https://doi.org/10.1007/BF00041384
  4. Cleary, A. L., Fowke, L. C., Wang, H. and John, P. C. L. (2002) The effect of ICK1, a plant cyclin-dependent kinase inhibitor, on mitosis in living plant cells. Plant Cell Re. 20, 814-820. https://doi.org/10.1007/s00299-001-0407-y
  5. Zhou, Y., Wang, H., Gilmer, S., Whitwill, S. and Fowke, L. C. (2003) Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana. Planta 216, 604-613.
  6. Cho, J. W., Par, S. C., Shin, E. A., Kim, C. K., Han, W., Sohn, S. I., Song, P. S. and Wang, M. H. (2004) Cyclin D1 and p22ack1 play opposite roles in plant growth and development. Biochem. Biophy. Res. Com. 324, 52-57. https://doi.org/10.1016/j.bbrc.2004.08.233
  7. Beemster, G. T. S., Fiorani, F. and Inze, D. (2003) Cell cycle: the key to plant growth control? Trends Plant Sci. 4, 154-158.
  8. Doerner, P., Jorgensen, J. E., You, R., Steppuhn, J. and Lamb, C. (1996) Control of root growth and development by cyclin expression. Nature 380, 520-523 https://doi.org/10.1038/380520a0
  9. Meijer, M. and Murray, J. A. H. (2000) The role and regulation of D-type cyclins in the plant cell cycle. Plant Mol. Biol. 43, 621-633. https://doi.org/10.1023/A:1006482115915
  10. De Veylder, L., de Almeida Engler, J., Burssens, S., Manevski, A., Lescure, B., Van Montagu, M., Engler, G. and Inze, D. (1999) A new D-type cyclin of Arabidopsis Arabidopsis thaliana expressed during lateral root primordia formation. Planta 203, 453-462.
  11. Soni, R., Carmichael, J. P., Shah, Z. H. and Murray, J. A. H. (1995) A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif. Plant Cell 7, 85-103. https://doi.org/10.1105/tpc.7.1.85
  12. Cockcroft, C. E., den Boer, B. G. W., Healy, J. M. S. and Murray, J. A. H. (2000) CycD control of growth rate in plants. Nature 405, 575-579. https://doi.org/10.1038/35014621
  13. Riou-Khamlichi, C., Huntley, R., Jacqmard, A. and Murray, J. A. H. (1999) Cytokinin activation of Arabidopsis cell division through a D-type cyclin. Science 283, 1541-1544 https://doi.org/10.1126/science.283.5407.1541
  14. Dewitte, W., Riou-Khamlichi, C., Scofield, S., Healy, J. M., Jacqmard, A., Kilby, N. J. and Murray, J. A. H. (2003) Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3. Plant Cell 15, 79-92. https://doi.org/10.1105/tpc.004838
  15. Sorrell, D. A., Combettes, B., Chaubet-Gigot, N., Gigot, C. and Murray, J. A. H. (1999) Distinct cyclin D genes show mitotic accumulation or constant levels of transcripts in tobacco bright yellow-2 cells. Plant Physiol. 119, 343-351. https://doi.org/10.1104/pp.119.1.343
  16. Oakenfull, E. A., Riou-Knamlichi, C. and Murray, J. A. H. (2002) Plant D-type cyclins and the control of G1 progression. Philo. Trans. Royal. Soc. London B. Biol. Sci. 357, 749-760. https://doi.org/10.1098/rstb.2002.1085
  17. Gutierrez, C. (1998) The retinoblastoma pathway in plant cell cycle. Curr. Opin. Plant Biol. 1, 492-497. https://doi.org/10.1016/S1369-5266(98)80041-1
  18. de Jager, S. M. and Murray, J. A. H. (1999) Retinoblastoma proteins in plants. Plant Mol. Biol. 41, 295-299. https://doi.org/10.1023/A:1006398232003
  19. Mironov, V., De Veylder, L., Van Montagu, M. and Inze, D. (1999) Cyclin-dependent kinases and cell division in plants: The nexus. Plant Cell 3, 29-41.
  20. Nakagami, H., Kawamura, K., Sugisaka, K., Sekine, M. and Shinmyo, A. (2002) Phosphorylation of Retinoblastoma- Related Protein by the Cyclin D/Cyclin- Dependent Kinase Complex Is Activated at the G1/S-Phase Transition in Tobacco. Plant Cell 14, 1847-1857. https://doi.org/10.1105/tpc.002550
  21. Boucheron, E., Healy, J. H. S., Bajon, C., Sauvanet, A., Rembur, J., Noin, M., Sekine, M., Riou Khamlichi, C., Murray, J. A. H., Van Onckelen, H. and Chriqui, D. (2005) Ectopic expression of Arabidopsis CYCD2 and CYCD3 in tobacco has distinct effects on the structural organization of the shoot apical meristem. J. Exp. Bot. 56, 807-812. https://doi.org/10.1093/jxb/eri075
  22. Hu, Y., Han, C., Mou, Z. and Li, J. (1999) Monitoring gene expression by cDNA array. Chin. Sci. Bull. 44, 441-444. https://doi.org/10.1007/BF02977884
  23. Wang, H., Zhou, Y., Gilmer, S., Whitwill, S. and Fowke, L. C. (2000) Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology. Plant J. 24, 613-623. https://doi.org/10.1046/j.1365-313x.2000.00899.x
  24. Zhou, Y., Wang, H., Gilmer, S., Whitwill, S. and Fowke, L. C. (2003) Effects of co-expressing the plant CDK inhibitor ICK1 and D-type cyclin genes on plant growth, cell size and ploidy in Arabidopsis thaliana. Planta. 216, 604-613.
  25. Zhou, Y., Fowke, L. C. and Wang, H. (2002) Plant CDK inhibitor: studies of interactions with cell cycle regulators in the yeast two-hybrid system and functional comparisons in transgenic Arabidopsis plants. Plant Cell Rep. 20, 967-975. https://doi.org/10.1007/s00299-001-0434-8
  26. Polymenis, M. and Schmidt, E. V. (1999) Coordination of cell growth with cell division. Curr. Opin. Genet. Dev. 9, 76-80. https://doi.org/10.1016/S0959-437X(99)80011-2
  27. Bartkova, J., Lukas, J., Müller, H., Lützhoft, D., Strauss, M. and Bartek, J. (1994) Cyclin D1 protein expression and function in human breast cancer. Int. J. Cancer 57, 353-361. https://doi.org/10.1002/ijc.2910570311
  28. Liang, S. B., Furihata, M., Takeuchi, T., Iwata, J., Chen, B. K., Sonobe, H. and Ohtsuki, Y. (2000) Overexpression of cyclin D1 in nonmelanocytic skin cancer. Virchows Arch. 436, 370-376. https://doi.org/10.1007/s004280050461
  29. Wang, T. C., Cardiff, R. D., Zukerberg, L., Lees, E., Arnold, A. and Schmidt, E. V. (1994) Mamary hyperplasia and carcinoma in MMTV-cyclin D1 transgenic mice. Nature 369, 669-671. https://doi.org/10.1038/369669a0
  30. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Planta 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  31. Ling, H., Zhao, J. Y., Zuo, K. J., Qiu, C. X., Yao, H. Y., Qin, J., Sun, X. F. and Tang, K. X. (2006) Isolation and Expression Analysis of a GDSL-like Lipase Gene from Brassica napus L. J. Biochem. Mol. Biol. 39, 297-303. https://doi.org/10.5483/BMBRep.2006.39.3.297
  32. Horsch, R. B., Fry, J. E., Hoffmann, N. L., Eichholtz, D., Rogers, S. G. and Fraley, R. T. (1985) A simple and general method for transferring genes into plants. Nature 227, 1229-1231. https://doi.org/10.1038/2271229a0
  33. Doyle, J. J. and Doyle, J. L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phyto Bull 19, 11-15.
  34. Yi, S. Y., Yu, S. H. and Choi, D. (1999) Molecular cloning of a catalase cDNA from Nicotiana glutinosa L. and its repression by tobacco mosaic virus infection. Mol. Cells 30, 320-325.

Cited by

  1. Overexpression of the anaphase-promoting complex (APC) genes in Nicotiana tabacum promotes increasing biomass accumulation vol.40, pp.12, 2013, https://doi.org/10.1007/s11033-013-2832-8
  2. CbCBF from Capsella bursa-pastoris enhances cold tolerance and restrains growth in Nicotiana tabacum by antagonizing with gibberellin and affecting cell cycle signaling vol.85, pp.3, 2014, https://doi.org/10.1007/s11103-014-0181-1
  3. The D-type cyclin gene (Nicta;CycD3;4) controls cell cycle progression in response to sugar availability in tobacco vol.168, pp.2, 2011, https://doi.org/10.1016/j.jplph.2010.06.004