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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Proteomic analysis of androgen-independent growth in low and high passage human LNCaP prostatic adenocarcinoma cells

  • Youm, Yun-Hee (Department of Urology, Eulji Medical Center, Eulji University School of Medicine) ;
  • Kim, Se-Yoon (Department of Biochemistry, Yonsei University) ;
  • Bahk, Young-Yil (Protein Network Research Center, Yonsei University) ;
  • Yoo, Tag-Keun (Department of Urology, Eulji Medical Center, Eulji University School of Medicine)
  • Published : 2008.10.31
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Abstract

The present study compared the proteomic characteristics of a low passage number (L-33) and high passage number (H-81) LNCaP cell clone. Marked differences in protein expression were noted in the response of L-33 and H-81 cells to androgens. To investigate if regulation of these proteins was androgen-dependent, expression of the androgen receptor was silenced via small interfering RNA. Consistent with the proteomic data, abrogation of androgen receptor production in H-81 cells resulted in the reversed expression level into L-33 cells compared with non-treated H-81 LNCaP cells. The results clarify the progression into an androgen-independent phenotype.

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References

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