Journal of Korean Society of Forest Science (한국산림과학회지)

Korean Society of Forest Science (한국산림과학회)
권호 표지

Study on Anti-inflammatory and Anti-microbial Effect of Pinus rigida Mill. inner Bark Extracts as a Cosmetic Material

리기다소나무(Pinus rigida Mill.) 내수피 추출물에 대한 화장품 소재로써의 항염 및 항균효과

  • Jang, Min-Jung (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Young-Hun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Chang-Eeon (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Kim, Sea-Hyun (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Byung-Guen (Department of Forest Resources, Yeungnam University) ;
  • Lee, Do-Hyung (Department of Forest Resources, Yeungnam University)
  • 장민정 (대구한의대학교 화장품약리학과) ;
  • 김영훈 (대구한의대학교 화장품약리학과) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 이창언 (대구한의대학교 화장품약리학과) ;
  • 이진태 (대구한의대학교 화장품약리학과) ;
  • 김세현 (국립산림과학원 산림유전자원부) ;
  • 이병근 (영남대학교 산림자원학과) ;
  • 이도형 (영남대학교 산림자원학과)
  • Received : 2008.01.23
  • Accepted : 2008.03.25
  • Published : 2008.06.29
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Abstract

Recently, there has been a great deal of interest in the applications of plant-based extracts to both cosmetic and medicinal industries. The objective of this study was to investigate the anti-inflammatory and antimicrobial effect of P. rigida extracts by water and ethyl acetate. Anti-inflammatory and anti-microbial effect of P. rigida extracts by water and EtOAc were investigated by using nitrite scavenging ability, nitric oxide production and anti-microbial ability. In the test of nitrite scavenging ability, P. rigida extracts by water and EtOAc showed 88.7% and 99% at 100 ppm concentration, respectively. The cell viability was measured using the MTT assay at 24 hours after P. rigida extracts as shown in over 80%. Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. NO productions in LPS and P. rigida extracts stimulated group were decreased in a concentration and were dependent on time as compared with LPS stimulated. The water extracts showed the highest inhibition at the 100 ppm concentration. In anti-microbial activity test, the water extract with 3.0 mg/disc resulted in the clear zone of 14 mm, and ethyl acetate with that of 15 mm for Staphylococcus aureus. However, P. rigida extracts didn't show any growth inhibitory effect on Esherichia coli. These results indicate that the extracts of P. rigida have anti-inflammatory activities as a cosmeceuticals.

최근 식물을 이용한 추출물이 화장품과 의약품에 많이 이용되고 있다. 이에 본 연구에서는 수목의 미활용 부위인 리기다소나무 내수피의 화장품소재로써 가능성을 검토하고자 연구하였다. 이를 위해 리기다소나무 내수피 추출물을 이용하여 아질산염 소거능, 항염효과, 항균효과를 분석하여 다음과 같은 결론을 얻었다. 아질산염 소거능 실험결과 100ppm에서 물가용부는 88.7%, EtOAc가용부는 99%로 대조군인 vitamin C와 유사한 소거작용을 나타내어 리기다소나무 내수피 추출물의 뛰어난 아질산염 소거능을 확인할 수 있었다. 시료의 NO 저해활성이 세포독성으로 인한 cell population의 저하에서 기인하는 것인지 관찰하기위해 MTT assay를 통해 cell의 생존율을 측정한 결과 80% 이상의 세포 생존율을 나타내어 이것을 토대로 NO 저해활성의 실험을 진행하였다. LPS로 활성화된 Raw 264.7 세포의 배양액 중에 생성된 nitrite양을 griess시약을 사용하여 NO생성 저해를 측정하였다. 물가용부와 EtOAc가용부 모든 농도에서 12시간부터 24시간까지 NO생성을 저해하였으며, 물가용부는 100ppm의 낮은 농도에서 뛰어난 NO생성 저해능을 나타내었다. 항균효과 측정 실험에서 그람양성균인 S. aureus에 대하여 물가용부 3mg/disc에서 14mm의 생육 저해환을 나타내었으며, EtOAc가용부는 3mg/disc에서 15mm의 생육 저해환을 나타내었다. 효모인 C. albicans에 대해서는 EtOAc가용부 3mg/disc에서 15mm의 생육 저해환을 나타내었으며, S. epidermidis와 그람음성균인 E. coli에서는 생육 저해환을 나타내지 않았다. 본 연구의 결과에서는 리기다소나무 내수피 추출물의 항염 및 항균효과와 천연 소재로서 화장품 산업에 활용 가능성을 확인할 수 있었다.

Keywords

Acknowledgement

Supported by : 영남대학교

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