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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants

  • Wu, Xiao-Liang (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Hou, Wen-Cui (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Wang, Mei-Mei (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Zhu, Xiao-Ping (College of Plant Protection, Shandong Agricultural University) ;
  • Li, Fang (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Zhang, Jie-Dao (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Li, Xin-Zheng (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University) ;
  • Guo, Xing-Qi (State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University)
  • Published : 2008.05.31
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Abstract

The discovery of RNA silencing inhibition by virus encoded suppressors or low temperature leads to concerns about the stability of transgenic resistance. RNA-dependent RNA polymerase (RdRp) has been previously characterized to be essential for transgene-mediated RNA silencing. Here we showed that low temperature led to the inhibition of RNA silencing, the loss of viral resistance and the reduced expression of host RdRp homolog (NtRdRP1) in transgenic T4 progeny with untranslatable potato virus Y coat protein (PVY-CP) gene. Moreover, RNA silencing and the associated resistance were differently inhibited by potato virus X (PVX) and tobacco mosaic virus (TMV) infections. The increased expression of NtRdRP1 in both PVX and TMV infected plants indicated its general role in response to viral pathogens. Collectively, we propose that biotic and abiotic stress factors affect RNA silencing-mediated resistance in transgenic tobacco plants and that their effects target different steps of RNA silencing.

Keywords

References

  1. Mourrain, P., Beclin, C., Elmayan, T., Feuerbach, F., Godon, C., Morel, J. B., Jouette, D., Lacombe, A. M., Nikic, S., Picault, N., Remoue, K., Sanial, M., Vo, T. A. and Vaucheret H. (2000) Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance. Cell 101, 533-542 https://doi.org/10.1016/S0092-8674(00)80863-6
  2. Chellappan, P., Vanitharani, R., Ogbe, F. and Fauquet, C. M. (2005) Effect of temperature on geminivirus-induced RNA silencing in plants. Plant Physiol. 138, 1828-1841 https://doi.org/10.1104/pp.105.066563
  3. Szittya, G., Silhavy, D., Molnar, A., Havelda, Z., Lovas, A., Lakators, L., Banfalvi, Z. and Burgyan, J. (2003) Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. EMBO J. 22, 633-640 https://doi.org/10.1093/emboj/cdg74
  4. Soosaar, J. L., Burch-Smith, T. M. and Dinesh-Kumar, S. P. (2005) Mechanisms of plant resistance to viruses. Nature 3, 789-798
  5. Xie, Z., Fan, B., Chen, C. and Chen, Z. (2001) An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense. Proc. Natl. Acad. Sci. U.S.A. 98, 6515-6521.
  6. Dalmay, T., Hamilton, A., Rudd, S., Angell, S. and Baulcombe, D. C. (2000) An RNA-dependent RNA polymerase gene in Arabidopsis is required for posttranscriptional gene silencing mediated by a transgene but not by a virus. Cell 101, 543-553 https://doi.org/10.1016/S0092-8674(00)80864-8
  7. Guo, X. Q., Han, H. Y., Zhang, J. D. and Wang, H. G. (2003) The viral resistance of transgenic tobacco plants containing untranslatable $PVY^N$ coat protein gene. Acta Biologiae Experimentalis Sinica 36, 176-184
  8. Guo, X. Q., Lu, S. E., Zhu, C. X., Song, Y. Z., Meng, X. B., Zheng, C. C. and Wen F. J. (2001) RNA-mediated viral resistance against potato virus Y (PVY) in transgenic tobacco plants. Acta Phytopathologica Sinica 31, 349-356
  9. Qu, F. and Morris, T. J. (2005) Suppressors of RNA silencing encoded by plant viruses and their role in viral infections. FEBS Lett. 579, 5958-5964 https://doi.org/10.1016/j.febslet.2005.08.041
  10. Hine, R. B., Osborne, W. E. and Dennis, R. E. (1970) Elevation and temperature effects on severity of maize dwarf mosaic virus in sorghum in Arizona. Plant Dis. Rep. 54, 1064-1068
  11. Mitter, N., Sulistyowati, E., Graham, M. W. and Diezgen, R. G. (2001) Suppression of gene silencing: A threat to virus- resistant transgenic plants? Trends Plant Sci. 6, 246-247 https://doi.org/10.1016/S1360-1385(01)01947-1
  12. Voinnet, O., Lederer, C. and Baulcombe, D. C. (2000) A viral movement protein prevents spread of the gene silencing signal in Nicotiana benthamiana. Cell 103, 157-167 https://doi.org/10.1016/S0092-8674(00)00095-7
  13. Vance, V. B. (1991) Replication of potato virus X RNA is altered in coinfections with potato virus Y. Virology 182, 486-494 https://doi.org/10.1016/0042-6822(91)90589-4
  14. Zhou, L., Zhang, J., Wang, X., Jiang, H., Xi, F. and Hu, Y. (2006) Expression and characterization of RNA-dependent RNA polymerase of Dendrolimus punctatus tetravirus. J. Biochem. Mol. Biol. 39, 571-577 https://doi.org/10.5483/BMBRep.2006.39.5.571
  15. Agrawal, N., Dasaradhi, P. V. N., Mohmmed, A., Malhotra, P., Bhatnagar, R. K. and Mukherjee, S. K. (2003) RNA Interference: biology, mechanism, and applications. Microbiol. Mol. Biol. Rev. 67, 657-685 https://doi.org/10.1128/MMBR.67.4.657-685.2003
  16. Hamilton, A., Voinnet, O., Chappell, L. and Baulcombe, D. C. (2002) Two classes of short interfering RNA in RNA silencing. EMBO J. 21, 4671-4679 https://doi.org/10.1093/emboj/cdf464
  17. Voinnet, O., Pinto, Y. M. and Baulcombe, D. C. (1999) Suppression of gene silencing: a general strategy used by div erse DNA and RNA viruses. Proc. Natl. Acad. Sci. U.S.A. 96, 14147-14152. https://doi.org/10.1073/pnas.96.24.14147
  18. Roth, B. M., Pruss, G. J. and Vance, V. B. (2004) Plant viral suppressors of RNA silencing. Virus Res. 102, 97-108 https://doi.org/10.1016/j.virusres.2004.01.020
  19. Silhavy, D. and Burgyan, J. (2004) Effects and side-effects of viral RNA silencing suppressors on short RNAs. Trends Plant Sci. 9, 76-83 https://doi.org/10.1016/j.tplants.2003.12.010
  20. Brigneti, G., Voinnet, O., Li, W. X., Ji, L. H., Ding, S. W. and Baulcombe, D. C. (1998) Viral pathogenicity determinants are suppressors of transgene silencing in Nicotiana benthamiana. EMBO J. 17, 6739-6746 https://doi.org/10.1093/emboj/17.22.6739
  21. Qu, F., Ye, X. H., Hou, G., Sato, S., Clemente, T. E. and Morris, T. J. (2005) RDR6 has a broad-spectrum but temperature- dependent antiviral defense role in Nicotiana benthamiana. J. Virol. 79, 15209-15217 https://doi.org/10.1128/JVI.79.24.15209-15217.2005
  22. Ding, X. S., Liu, J., Cheng, N. H., Folimonov, A., Hou, Y. M., Bao, Y., Katagi, C., Carter, S. A. and Nelson, R. S. (2004) The tobacco mosaic virus 126-kDa protein associated with virus replication and movement suppresses RNA silencing. Mol. Plant Microbe. Interact. 17, 583-592 https://doi.org/10.1094/MPMI.2004.17.6.583
  23. Clark, M. F. and Adams, A. N. (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J. Gen. Virol. 34, 475-483 https://doi.org/10.1099/0022-1317-34-3-475
  24. Guo, X. Q., Wen, F. J., Song, Y. Z., Meng, X. B., Lu, S. E., Zheng, C. C. and Zhu, C. X. (2001) Expression of translatable and untranslatable potato virus Y ($PVY^N$) coat protein (CP) genes in tobacco and comparison of virus resistance between the transgenic plants. Chinese J. Virol. 17, 360-367

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