Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP-HPLC

RP-HPLC를 이용한 감초에서 liquiritin, glycyrrhizic acid, glabridin의 분리

  • Tian, Minglei (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University) ;
  • Yan, Hongyuan (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University) ;
  • Row, Kyung Ho (Center for Advanced Bioseperation Technology, Department of Chemical Engineering, Inha University)
  • 전명래 (초정밀생물분리기술연구센터, 화공과, 인하대학교) ;
  • 염홍원 (초정밀생물분리기술연구센터, 화공과, 인하대학교) ;
  • 노경호 (초정밀생물분리기술연구센터, 화공과, 인하대학교)
  • Received : 2007.11.19
  • Accepted : 2008.02.21
  • Published : 2008.04.25
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Abstract

Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.

역상고성능액상크로마토그래피(RP-HPLC)를사용하여감초에포함된 liquiritin (LQ), glycyrrhizic acid (GA)과 glabridin을 분석하였다. 이동상의 조성에 대한 실험을 수행한 실험결과에 의하면, 최적화된 이동상은 메탄올-물 이성분계이다. 이동상의 유량은 1 mL/min으로 일정하였고, 초기 8분동안에 35/65 (MeOH/water, v/v%)에서 80/20으로 구배용매조성법을 적용하였고, 그 이후에는 80/20으로 유지하였다. LQ과 GA의 농도는 0.2 mg/mL에서 1.0 mg/mL까지, glabridin의 농도는 0.01 mg/mL에서 0.2 mg/mL의 범위에서 선형성을 보였고, 상대 표준편차는 0.90%(n=5) 보다 낮았다. 개발된 방법을 사용하여 감초에 포함된위의세가지성분의정량분석을성공적으로수행하였다. 평균수율은 LQ가 80.79%, GA가 89.71%, glabridin이 72.50%이었다.

Keywords

References

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