Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Radioprotective effect of fucoidan against hematopoietic and small intestinal stem cells of γ-ray irradiated mice

감마선을 조사한 마우스의 조혈 및 소장줄기세포에 대한 fucoidan의 방호효과

  • Park, Eunjin (College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Jeon, Seong Mo (College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Joo, Hong-Gu (College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Hwang, Kyu-Kye (College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University) ;
  • Jee, Youngheun (College of Veterinary Medicine and Applied Radiological Science Research Institute, Cheju National University)
  • Accepted : 2008.12.05
  • Published : 2008.12.30
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Abstract

We investigated the potential of fucoidan for its ability to provide protection from gamma rayinduced damage. In our results, the fucoidan significantly improved the counts of endogenous colony forming unit to $9.5 {\pm} 1.5$, from $5.5 {\pm} 2.5$ compared with un-treated irradiated control group at 10 day after 7 Gy whole body irradiation. After 2 Gy irradiation, fucoidan treatment attenuated the percent of tail DNA of splenocytes, parameters of DNA damage, from $30.17 {\pm} 1.7%$ to $13.67 {\pm} 2.81%$ 2.81% by comet assay and also accelerated the proliferation of splenocytes, compared with un-treated irradiated control group by 3Hthymidine incorporation assay. Furthermore, fucoidan decreased the number of apoptotic fragments per intestinal crypt by 31.8% at 1 days after 2 Gy irradiation. These results indicated that the fucoidan significantly improved the hematopoietic recovery, prevented the DNA damage in immune cells and enhanced their proliferation, which had been suppressed by ionizing radiation. in addition, fucoidan rescued intestinal cells from radiation-induced apoptosis. Thus, this study raises the possibility of using fucoidan as adjuvant therapeutic agent after radiotherapy.

Keywords

References

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