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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Identification of marbling-related candidate genes in M. longissimus dorsi of high- and low marbled Hanwoo (Korean Native Cattle) steers

  • Lee, Seung-Hwan (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Cho, Yong-Min (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Lee, Sang-Hong (Genetic Improvement and Evaluatiion Division, National Institute of Animal Science, RDA) ;
  • Kim, Bum-Soo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Kim, Nam-Kuk (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Choy, Yeon-Ho (Hanwoo Experiment Station, National Institute of Animal Science, RDA) ;
  • Kim, Kyoung-Hoon (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Yoon, Du-Hak (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Im, Seok-Ki (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Oh, Sung-Jong (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA)) ;
  • Park, Eung-Woo (Animal Genomics & Bioinformatics Division, National Institute of Animal Science, Rural Development Administration (RDA))
  • Published : 2008.12.31
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Abstract

This study was conducted to identify marbling-related candidate genes in M. longissimus dorsi of high- and low-marbled Hanwoo. The longissimus dorsi muscles were selected for gene expression from eight Hanwoo steer carcasses based on crude fat content. In the analysis of variance, gene expression of five candidate genes, FABP4, SCD, $PPAR\gamma$, Titin and Nebulin was determined to be significantly different between high- and low-marbled Hanwoo steers (P < 0.0001). The Pik-4 and CaMK II genes were also shown to have a significant effect on crude fat content (P < 0.01). In the analysis of the differential expression between high- and low marbled groups, FABP4 gene expression was approximately 2 times higher in the high marbled group relative to the low marbled group. However, the $PPAR\gamma$ and SCD gene were highly expressed in the low marbled group. In addition, Titin and Nebulin were highly expressed in the low marbled group when placed under relatively high shear force. Finally, the Pik-4 and CaM K II gene also displayed a high expression pattern in the low marbled group.

Keywords

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