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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Examination of specific binding activity of aptamer RNAs to the HIV-NC by using a cell-based in vivo assay for protein-RNA interaction

  • Jeong, Yu-Young (National Research Laboratory for Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea) ;
  • Kim, Seon-Hee (National Research Laboratory for Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea) ;
  • Jang, Soo-In (National Research Laboratory for Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea) ;
  • You, Ji-Chang (National Research Laboratory for Molecular Virology, Department of Pathology, College of Medicine, The Catholic University of Korea)
  • Published : 2008.07.31
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Abstract

The nucleocapsid (NC) protein of the Human Immunodeficiency Virus-1 plays a key role in viral genomic packaging by specifically recognizing the Psi($\Psi$) RNA sequence within the HIV-1 genome RNA. Recently, a novel cell-based assay was developed to probe the specific interactions in vivo between the NC and $\Psi$-RNA using E.coli cells (J. Virol. 81: 6151-55, 2007). In order to examine the extendibility of this cell-based assay to RNAs other than $\Psi$-RNA, this study tested the RNA aptamers isolated in vitro using the SELEX method, but whose specific binding ability to NC in a living cellular environment has not been established. The results demonstrate for the first time that each of those aptamer RNAs can bind specifically to NC in a NC zinc finger motif dependent manner within the cell. This confirms that the cell-based assay developed for NC-$\Psi$interaction can be further extended and applied to NC-binding RNAs other than $\Psi$-RNA.

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References

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