A Study for Verification of Hair Growth Effect of Azelaic Acid and Vitamin B6

아젤라인산 및 비타민 B6의 육모효과 검증에 관한 연구

  • Yoon, Sean Hyuck (Department of Plastic and Reconstructive Surgery, Collage of Medicine, Daegu Catholic University) ;
  • Park, Dae Hwan (Department of Plastic and Reconstructive Surgery, Collage of Medicine, Daegu Catholic University) ;
  • Sin, Jeong Im (Tissue Engineering Center, Collage of Medicine, Daegu Catholic University)
  • 윤신혁 (대구가톨릭대학교 의과대학 성형외과학교실) ;
  • 박대환 (대구가톨릭대학교 의과대학 성형외과학교실) ;
  • 신정임 (대구가톨릭대학교 의과대학 조직공학센터)
  • Received : 2008.05.12
  • Accepted : 2008.07.14
  • Published : 2008.10.15

Abstract

Purpose: Interest in the augmentation of hair growth for functional and aesthetic purpose has increased dramatically in recent years. Many hair growth products have been released, but most of these have not been proven scientifically. This study aims to measure the hair growth effect of azelaic acid and vitamin $B_6$, which have been known as hair growth materials, in animal models. Methods: Six weeks old C57BL/6 mice were used in this study and hair of mice were removed by topical treatment. The mice were divided into five experimental groups according to the testing material such as saline (negative control), propylene glycol(vehicle control), azelaic acid, vitamin B6 and azelaic acid plus vitamin B6 in combination. Hair growth was documented photographically and histologically, and then analysed by the high quality hair analysis program system. The quantity of endocrine factors, IGF-I and TGF-${\beta}1$ in the skin of mice was measured by PCR analysis. Results: The topical treatment of azelaic acid and vitamin B6 in combination for 2 weeks to dorsal skin accelerated hair regrowth more than other groups. The azelaic acid and vitamin $B_6$-combined treatment also promoted hair follicle elongation and thickness compared to the others. Histologic studies showed increased number of basal cells in azelaic acid and vitamin $B_6$-combined treatment. Furthermore, the azelaic acid and vitamin $B_6$-combined group significantly increased the expression of IGF-I but decreased the expression of TGF-${\beta}1$ in the skin of mice compared to other groups. Conclusion: These results suggest that azelaic acid and vitamin $B_6$, when used together, have an additive effect and might be used as hair growth materials.

Keywords

References

  1. Drake L, Hordinsky M, Fieldler V, Swinehart J, Unger WP, Cotterill PC, Thiboutot DM, Lowe N, Jacobson C, Whiting D, Stieglitz S, Kraus SJ, Griffin EI, Weiss D, Carrington P, Gencheff C, Cole GW, Pariser DM, Epstein ES, Tanaka W, Dallob A, Vandormael K, Geissler L, Waldstreicher J: The effects of finasteride on scalp skin and serum androgen levels in men with androgenetic alopecia. J Am Acad Dermatol 41: 550, 1999
  2. Olsen EA, Dunlap FE, Funicella T, Koperski JA, Swinehart JM, Tschen EH, Trancik RJ: A randomized clinical trial of 5% topical minoxidil versus 2% topical minoxidil and placebo in the treatment of androgenetic alopecia in men. J Am Acad Dermatol 47: 377, 2002 https://doi.org/10.1067/mjd.2002.124088
  3. Yang JS, Lavker RM, Sun TT: Upper human hair follicle contains a subpopulation of keratinocytes with superior in vitro proliferative potential. J Invest Dermatol 101: 652, 1993 https://doi.org/10.1111/1523-1747.ep12371671
  4. Yamamoto S, Jiang H, Kato R: Stimulation of hair growth by topical application of FK506, a potent immunosuppressive agent. J Invest Dermatol 102: 160, 1994 https://doi.org/10.1111/1523-1747.ep12371755
  5. Paus R, Stenn KS, Link RE: Telogen skin contains an inhibitor of hair growth. Br J Dermatol 122: 777, 1990 https://doi.org/10.1111/j.1365-2133.1990.tb06266.x
  6. Dargie HJ, Dollery CT, Daniel J: Minoxidil in resistant hypertension. Lancet 2: 515, 1977
  7. Frampton JE, Wagstaff AJ: Azelaic acid 15% gel in the treatment of papulopustular rosacea. Am J Clin Dermatol 5: 57, 2004 https://doi.org/10.2165/00128071-200405010-00009
  8. Krautheim A, Gollnick H: Transdermal penetration of topical drugs used in the treatment of acne. Clin Pharmacokinet 42: 1287, 2003 https://doi.org/10.2165/00003088-200342140-00005
  9. Sasmaz S, Arican O: Comparison of azelaic acid and anthralin for the therapy of patchy alopecia areata: a pilot study. Am J Clin Dermatol 6: 403, 2005 https://doi.org/10.2165/00128071-200506060-00007
  10. Messenger AG: The control of hair growth and pigmentation. New York, McGraw-Hill, 1994, p 39
  11. Mitsui S, Ohuchi A, Hotta M, Tsuboi R, Ogawa H: Genes for a range of growth factors and cyclin- dependent kinase inhibitors are expressed by isolated human hair follicles. Br J Dermatol 137: 693, 1997 https://doi.org/10.1111/j.1365-2133.1997.tb01103.x
  12. Tsuji Y, Denda S, Soma T, Raftery L, Momoi T, Hibino T: A potential suppressor of TGF-$\beta$ delays catagen progression in hair follicles. J Investig Dermatol Symp Proc 8: 65, 2003
  13. Lurie R, Ben-Amitai D, Laron Z: Laron syndrome (primary growth hormone insensitivity): a unique model to explore the effect of insulin-like growth factor 1 deficiency on human hair. Dermatology 208: 314, 2004 https://doi.org/10.1159/000077839
  14. Jiang H, Yamamoto S, Kato R: Induction of anagen in telogen mouse skin by topical application of FK506, a potent immunosuppressant. J Invest Dermatol 104: 523, 1995 https://doi.org/10.1111/1523-1747.ep12606018