BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Expression of CD320 in human B cells in addition to follicular dendritic cells

  • Cho, Wha-Jung (Department of Microbiology and Immunology, Kangwon National University College of Medicine) ;
  • Choi, Jin-Suk (Department of Microbiology and Immunology, Kangwon National University College of Medicine) ;
  • Park, Chan-Hum (Department of Otorhinolaryngology, Hallym University College of Medicine) ;
  • Yoon, Sun-Ok (Laboratory of Cellular Immunology, Ochsner Clinic Foundation) ;
  • Jeoung, Doo-Il (Vascular System Research Center, Kangwon National University) ;
  • Kim, Young-Myeong (Vascular System Research Center, Kangwon National University) ;
  • Choe, Jong-Seon (Department of Microbiology and Immunology, Kangwon National University College of Medicine)
  • Published : 2008.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

CD320 has been recently discovered and reported as a follicular dendritic cell (FDC) protein. Although CD320 is known to enhance proliferation of germinal center (GC) B cells, little other information is available. In this study, we investigated its cellular distribution in the GC. Confocal microscopy of human tonsil sections revealed co-localization of CD320 with CD19 and CD38 but not with CD3 indicating that GC B cells expressed CD320 in addition to FDC. In purified GC B cells, CD320 expression was inhibited in the nucleus, membrane and cytoplasm. Reverse transcriptase-polymerase chain reaction confirmed CD320 mRNA expression in B cells. These finding indicate that CD320 is expressed in B cells in addition to FDC, and that its GC activity may be more complicated than previously thought.

Keywords

References

  1. MacLennan, I. C. M. (1994) Germinal Centers. Annu. Rev. Immunol. 12, 117-139. https://doi.org/10.1146/annurev.iy.12.040194.001001
  2. Nieuwenhuis, P. and Opstelten, D. (1984) Functional anatomy of germinal centers. Am. J. Anat. 170, 421-435. https://doi.org/10.1002/aja.1001700315
  3. Berek, C., Berger, A. and Apel, M. (1991) Maturation of the immune response in germinal centers. Cell 67, 1121-1129. https://doi.org/10.1016/0092-8674(91)90289-B
  4. Cosgrove, D., Gray, D., Dierich, A., Kaufman, J., Lemeur, M., Benoist, C. and Mathis, D. (1991) Mice lacking MHC class II molecules. Cell 66, 1051-1066. https://doi.org/10.1016/0092-8674(91)90448-8
  5. Kawabe, T., Naka, T., Yoshida, K., Tanaka, T., Fujiwara, H., Suematsu, S., Yoshida, N., Kishimoto, T. and Kikutani, H. (1994) The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation. Immunity 1, 167-178. https://doi.org/10.1016/1074-7613(94)90095-7
  6. Moller, P., Henne, C., Leithauser, F., Eichelmann, A., Schmidt, A., Bruderlein, S., Dhein, J. and Krammer, P.H. (1993) Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells and in follicle center and mediastinal B-cell lymphomas. Blood 81, 2067-2075.
  7. Koopman, G., Keehnen, R. M. J., Lindhout, E., Newman, W., Shimizu, Y., van Seventer, G. A., de Groot, C. and Pals, S. T. (1994) Adhesion through the LFA-1 (CD11a/ CD18)-ICAM-1 (CD54) and the VLA-4 (CD49d)-VCAM-1 (CD106) pathways prevents apoptosis of germinal center B cells. J. Immunol. 152, 3760-3767.
  8. Nagata, S. and Golstein, P. (1995) The Fas death factor. Science 267, 1449-1455. https://doi.org/10.1126/science.7533326
  9. Pasparakis, M., Alexopoulou, L., Episkopou, V. and Kollias, G. (1996) Immune and inflammatory responses in TNFa-deficient mice: a critical requirement for TNFa in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J. Exp. Med. 184, 1397-1411. https://doi.org/10.1084/jem.184.4.1397
  10. Matsumoto, M., Lo, S. F., Carruthers, C. J. L., Min, J., Mariathasan, S., Huang, G., Plas, D. R., Martin, S. M., Geha, R. S., Nahm, M. H. and Chaplin, D. D. (1996) Affinity maturation without germinal centres in lymphotoxin- a-deficient mice. Nature 382, 462-466. https://doi.org/10.1038/382462a0
  11. Li, L., Zhang, X., Kovacic, S., Long, A. J., Bourque, K., Wood, C. R. and Choi, Y. S. (2000) Identification of a human follicular dendritic cell molecule that stimulates germinal center B cell growth. J. Exp. Med. 191, 1077-1083. https://doi.org/10.1084/jem.191.6.1077
  12. Florian, S., Sonneck, K., Czerny, M., Hennersdorf, F., Hauswirth, A. W., Buhring, H.-J. and Valent, P. (2006) Detection of novel leukocyte differentiation antigens on basophils and mast cells by HLDA8 antibodies. Allergy 61, 1054-1062. https://doi.org/10.1111/j.1398-9995.2006.01171.x
  13. Zhang, X., Li, L., Jung, J., Xiang, S., Hollmann, C. and Choi., Y. S. (2001) The distinct roles of T cell-derived cytokines and a novel follicular dendritic cell-signaling molecule 8D6 in germinal center-B cell differentiation. J. Immunol. 167, 49-56. https://doi.org/10.4049/jimmunol.167.1.49
  14. Li, L., Yoon, S.-O., Fu, D.-D., Zhang, X. and Choi, Y. S. (2004) Novel follicular dendritic cell molecule, 8D6, collaborates with CD44 in supporting lymphomagenesis by a Burkitt lymphoma cell line, L3055. Blood 104, 815-821. https://doi.org/10.1182/blood-2004-01-0292
  15. Liu, Y.-J., Xu, J., de Bouteiller, O., Parham, C. L., Grouard, G., Djossou, O., de Saint-Vis, B., Lebecque, S., Banchereau, J. and Moore, K. W. (1997) Follicular dendritic cells specifically express the long CR2/CD21 isoform. J. Exp. Med. 185, 165-170. https://doi.org/10.1084/jem.185.1.165
  16. Dingwall, C. and Laskey, R. A. (1991) Nuclear targeting sequences--a consensus? Trends Biochem. Sci. 16, 478-481. https://doi.org/10.1016/0968-0004(91)90184-W
  17. Lee, I. Y., Lee, J., Park, W. S., Nam, E.-C., Shin, Y. O. and Choe, J. (2001) 3C8, a new monoclonal antibody directed against a follicular dendritic cell line, HK. Immune Network 1, 26-31 https://doi.org/10.4110/in.2001.1.1.26
  18. Choe, J., Kim, H.-S., Armitage, R. J. and Choi, Y. S. (1997) The functional role of BCR stimulation and IL-4 in the generation of human memory B cells from germinal center B cells. J. Immunol. 159, 3757-3766.
  19. Lee, I. Y., Cho, W., Kim, J., Park, C. S. and Choe, J. (2008) Human follicular dendritic cells interact with T cells via expression and regulation of cyclooxygenases and prostaglandin E and I synthases. J. Immunol. 180, 1390-1397. https://doi.org/10.4049/jimmunol.180.3.1390
  20. Zhang, P., Li, L., Hu, C., Xu, Q., Liu, X. and Qi, Y. (2005) Interactions among measles virus hemagglutinin, fusion protein and cell receptor signaling lymphocyte activation molecule (SLAM) indicating a new fusion-trimer model. BMB Rep. 38, 373-380. https://doi.org/10.5483/BMBRep.2005.38.4.373
  21. Hwang, H., Choi, S.Y. and Kim, T.Y. (2007) IL-4 suppresses UVB-induced apoptosis in skin. BMB Rep. 40, 36-43. https://doi.org/10.5483/BMBRep.2007.40.1.036

Cited by

  1. Vitamin B12 transport from food to the body's cells—a sophisticated, multistep pathway vol.9, pp.6, 2012, https://doi.org/10.1038/nrgastro.2012.76