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토양으로부터 분리한 Pandoraea sp. BCNU 315 에 관한 연구

Study on Pandoraea sp. BCNU 315 Isolated from Soil

  • 김선아 (창원대학교 생물학과) ;
  • 최혜정 (창원대학교 생명공학협동과정) ;
  • 우승희 (창원대학교 생명공학협동과정) ;
  • 황민정 (창원대학교 생물학과) ;
  • 박미란 (창원대학교 생명공학협동과정) ;
  • 김동완 (창원대학교 미생물학과) ;
  • 문자영 (창원대학교 보건의과학과) ;
  • 주우홍 (창원대학교 생물학과)
  • Kim, Seon-A (Department of Biology, Changwon National University) ;
  • Choi, Hye-Jung (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Woo, Seung-Hee (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Hwang, Min-Jung (Department of Biology, Changwon National University) ;
  • Park, Mi-Ran (Interdisciplinary Program in Biotechnology, Changwon National University) ;
  • Kim, Dong-Wan (Department of Microbiology, Changwon National University) ;
  • Moon, Ja-Young (Department of Biochemistry and Health Sciences, Changwon National University) ;
  • Joo, Woo-Hong (Department of Biology, Changwon National University)
  • 발행 : 2008.02.28

초록

안산지역의 하천 침전 토양에서 식물병원성진균에 대한 길항능력이 좋은 세균을 분리하였다. 이들 중 한 균주가 Rhizoctonia solani, Botrytis cenerea와 Fusarium oxysporum의 생장을 저해하는 활성을 보였다. 이 균주는 표현 형질과 분자계통학적인 특징에 의거하여 Pandoraea sp.로 동정되었다. 그리고 생육 최적 조건을 검토한 결과 tryptone과 sucrose가 각각 최적 질소원과 탄소원임을 확인할 수 있었으며, 최적 pH와 온도는 각각 pH 7.0 와 $30^{\circ}C$임이 확인되었다. Pandoraea sp. BCNU 315가 생성하는 물질들은 column chromatography로 정제하였고 고속액체크로마토그래피(HPLC), GC-MS 그리고 핵자기공명(NMR) 장치로 구조를 분석하였다. 그 결과 한 화합물은 indole이었으며 또 다른 화합물은 cyclopentadecaheptehe임이 추정되었다. 한편 Pandoraea sp. BCNU 315가 생성하는 6개의 화합물 중 나머지 4개 화합물의 자세한 구조 해석은 추후 연구를 통하여 밝혀져야 할 것이다.

Bacteria that antagonize plant pathogenic fungi were isolated from the sediment soil at the Ansan industrial estate. One isolate of them showed growth inhibition of Rhizoctonia solani, Botrytis cenerea, and Fusarium oxysporum. This strain was identified as Pandoraea sp. based on phenotypic and phylogenetic characteristics and termed Pandoraea sp. BCNU 315. Tryptone as nitrogen source and sucrose as carbon source were found to be most effective for the microbial growth. In addition, the optimum temperature and pH for microbial growth were $30^{\circ}C$ and pH 7.0, respectively. The substances generated from Pandoraea sp. BCNU 315 were purified and analyzed by column chromatography, HPLC, GC-MS and NMR. As a result, one compound was determined to be indole, another compound was predicted as cyclopentadecaheptene. Detailed structural clarification of the all of the rest six compounds from Pandoraea sp. BCNU 315 has to be accompanied in the further studies.

키워드

참고문헌

  1. Arima, K., H. Imanaka, M. Kousaka, A. Kousaka, A. Fukuta and G. Tamura. 1964. Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas. Agric. Biol. Chem. 28, 575-576 https://doi.org/10.1271/bbb1961.28.575
  2. Brian, P. W., J. M. Wright, J. Stubs and A. M. Way. 1951. Uptake of antibiotic metabolites of soil microorganisms by plant. Nature 167, 347-349 https://doi.org/10.1038/167347a0
  3. Coenye, T., E. Falsen, B. Hoste, M. Ohlen, J. Goris, J. R. W. Govan, M. Gillis and P. Vandamme. 2000. Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov. Int. J. Syst. Bacteriol. 50, 887-899 https://doi.org/10.1099/00207713-50-2-887
  4. Gomez-Gil, L., P. Kumar and D. Barriault. 2007. Characterization of biphenyl dioxygenase of Pandoraea pnomenusa B-356 as a potent polychlorinated biphenyl-degrading enzyme. J. Bacteriol. 189, 5705-5715 https://doi.org/10.1128/JB.01476-06
  5. Gregory, K. F., O. N. Allen, A. J. Riker and W. H. Peterson. 1952. Antibiotics as agents for the control of certain damping-off fungi. Am. J. Botany 9, 405-415
  6. Hector, R. F. and D. Pappagianis. 1983. Inhibition of chitin synthesis in the cell wall of Coccidioides immitis by polyoxin D. J. Bacteriol. 154, 488-498
  7. Kasahara, A., T. Izumi, T. Kikuchi and L. Xiao-Ping. 1987. 5-Nitroindole. J. Heterocyl. Chem. 24, 1555-1556 https://doi.org/10.1002/jhet.5570240611
  8. Kim, Y. S. and S. D. Kim. 1994. Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biocontrol agent. J. Microbiol. Biotech. 4, 296-304
  9. Lee, E. T. and S. D. Kim. 1999. Isolation and antifungal activity of the chitinase producing bacterium Serratia sp. 3059 as antagonistic bacterium against Fusarium sp. Agric. Chem. Biotechnol. 42, 181-187
  10. Lee, E. T. and S. D. Kim. 1999. Purification and characterization of antifungal chitinase from indigenous antagonistic microorganism Serratia sp. 3059. Agri. Chem. Biotechnol. 42, 7-11
  11. Leoffler, W., J. S. M. Tschen, N. Vanittanakom, M. Kugler, E. Knorpp, T. F. Hsieh and T. G. Wu. 1986. Antifungal effects of bacilysin and fengymycin from Bacillus subtilis F-29-3: a comparison with activities of other Bacillus antibiotics. J. Phytopathol. 115, 204-213 https://doi.org/10.1111/j.1439-0434.1986.tb00878.x
  12. Levitan, A. 1967. In vitro antibacterial activity of kasugamycin. Appl. Microbiol. 15, 750-753
  13. Matsui, T., T. Yoshida, T. Yoshimura and T. Nagasawa. 2006. Regioselective carboxylation of 1,3-dihydroxybenzene by 2,6-dihydroxybenzoate decarboxylase of Pandoraea sp. 12B-2. Appl. Microbiol. Biotechnol.. 73, 95-102 https://doi.org/10.1007/s00253-006-0437-z
  14. Moore, J. E., T. Coenye, P. Vandamme and J. Stuart Elborn. 2001. First report of Pandoraea norimbergensis isolated from food - potential clinical significance. Food Microbiol. 18, 113-114 https://doi.org/10.1006/fmic.2000.0375
  15. Nomoto, S. and A. Shimoyama. 2001. First synthesis of blastidic acid, a component amino acid in an antibiotic, blasticidin S. Tetrahedron. Lett. 42, 1753-1755 https://doi.org/10.1016/S0040-4039(01)00010-7
  16. Okeke, B. C., T. Siddique, M. C. Arbestain and W. T. Frankenberger. 2002. Biodegradation of $\gamma$-hexachlorocyclohexane (lindane) and $\alpha$- hexachlorocyclohexane in water and a soil slurry by a Pandoraea Species. J. Agric. Food Chem. 50, 2548 -2555 https://doi.org/10.1021/jf011422a
  17. Paulitz, T. C. and J. E. Loper. 1991. Lack of a role for fluorescent siderophore production in the biological control of Phythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathol. 81, 930-935 https://doi.org/10.1094/Phyto-81-930
  18. Schlumbaum, A., F. Mauch, U. Vogeli and T. Boker. 1986. Plant chitinase are potent inhibitors of fungal growth. Nature 324, 365-367 https://doi.org/10.1038/324365a0
  19. Takeuchi, S., K. Hirayama, K. Ueda, H. Sasaki and H. Yonehara. 1958. Blasticidin S, a new antibiotic. J. Antibiot. 11, 1-5
  20. Vandamme, P., J. Goris, T. Coenye, B. Hoste, D. Janssens, K. Kersters, P. DeVos and E. Falsen. 1999. Assignment of centers for disease control group IVc-2 to the genus Ralstonia as Ralstonia paucula sp. nov. Int. J. Syst. Bacteriol. 49, 663-669 https://doi.org/10.1099/00207713-49-2-663