Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Reactive Oxygen Species and Cytotoxicity of Bamboo (Phyllostachys pubescens) Sap

대나무수액의 활성산소 소거활성과 세포독성

  • Cho, Sook-Hyun (Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Choi, Yong-Jo (Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Rho, Chi-Woong (Gyeongsangnam-do Agricultural Research & Extension Services) ;
  • Choi, Chul-Yung (Division of Food Science, Jinju International University) ;
  • Kim, Deok-Song (Department of Biology, College of Natural Science, Chonnam National University) ;
  • Cho, Sung-Hwan (Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
  • 조숙현 (경상남도농업기술원) ;
  • 최용조 (경상남도농업기술원) ;
  • 노치웅 (경상남도농업기술원) ;
  • 최철웅 (국제대학교 식품과학부) ;
  • 김덕송 (전남대학교 생물학과) ;
  • 조성환 (경상대학교 식품공학과 및 농업생명과학연구원)
  • Published : 2008.02.28
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Abstract

The antioxidant properties of bamboos sap isolated from Phyllostachys pubescens were investigated. This product scavenged intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. The radical scavenging activity of bamboo sap protected the viability of peritoneal macrophage cells exposed to hydrogen peroxide $(H_2O_2)$, Furthermore, bamboo sap reduced apoptotic cell formation induced by $H_2O_2$ as demonstrated by decreases in the number of hypo-diploid cells am apoptotic cell body formation. These results indicate that bamboo sap has radical scavenging activity and ameliorates $H_2O_2$ induced cytotoxicity.

대나무수액의 자유라디칼 소거능을 측정하기 위하여 DPPH를 이용한 자유 라디칼 소거능 실험을 수행하여 항산화 효과를 측정한 결과 대나무수액의 농도가 높을수록 DPPH 활성이 뛰어남을 알 수 있었고, ROS를 이용하여 항산화효과를 확인하였다. 배양된 대식세포에 대나무수액을 농도별로 첨가한 결과 대나무수액 농토가 높을수록 과산화수소에 의해 유도된 산화적 자극이 감소하였다. 또한 세포 생존에 미치는 영향을 알고자 대나무수액을 농도별로 첨가하여 24시간 후 세포의 형태변화를 HIT assay로 실시한 결과 산화적 자극에 의해 발생한 세포손상이 대나무수액의 농도가 높아질수록 감소하는 것이 확인되었다. Ascorbic acid는 $H_2O_2$에 의해 야기되는 세포독성을 억제해 주는 효과가 10 %로 우수하게 나타났고 대나무수액 역시 고농도에서 27 %의 세포손상 방지효과가 우수하였다. 따라서 대나무수액은 안전하고 독성이 전혀 없는 천연 항산화제로서의 가능성을 보였다.

Keywords

References

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