Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Colchicine-Induced Polyploidy and It's Agronomic Characters in Bupleurum falcatum

배수체 작성에 따른 시호 작물 특성

  • Son, Tae-Kwon (R&D Center, ISTECH, Inc., CU Techno Center, Catholic University of Daegu) ;
  • Lee, Sang-Chul (Division of Biosciences, College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Chung, Il-Kyung (Department of Biotechnology, Catholic University of Daegu)
  • 손태권 ((주)이즈텍 기술연구소) ;
  • 이상철 (경북대학교 농업생명과학대학) ;
  • 정일경 (대구가톨릭대학교 생명공학과)
  • Published : 2008.02.29
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Abstract

The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.

Keywords

References

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