The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Gene Expression Profiling in Rice Infected with Rice Blast Fungus using SAGE

  • Kim, Sang-Gon (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kim, Sun-Tae (Environmental Biotechnology National Core Research Center, Gyeongsang National University) ;
  • Kim, Sung-Kun (Department of Chemistry and Biochemistry and Institute of Biomedical Studies, Baylor University) ;
  • Kang, Kyu-Young (Environmental Biotechnology National Core Research Center, Gyeongsang National University)
  • Published : 2008.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Rice blast disease, caused by the pathogenic fungus Magnaporthe grisea, is a serious issue in rice (Oryza sativa L.) growing regions of the world. Transcript profiling in rice inoculated with the fungus has been investigated using the transcriptomics technology, serial analysis of gene expression (SAGE). Short sequence tags containing sufficient information which are ten base-pairs representing the unique transcripts were identified by SAGE technology. We identified a total of 910 tag sequences via the GenBank database, and the resulting genes were shown to be up-regulated in all functional categories under the fungal biotic stress. Compared to the compatible interaction, the stress and defense genes in the incompatible interaction appear to be more up-regulated. Particularly, thaumatin-like gene (TLP) was investigated in determining the gene and protein expression level utilizing Northern and Western blotting analyses, resulting in an increase in both the gene and the protein expression level which arose earlier in the incompatible interaction than in the compatible interaction.

Keywords

References

  1. Angelastro, J. M., Klimaschewski, L. P. and Vitolo, O. V. 2000. Improved NlaIII digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols. Nucleic Acids Res. 28:E62 https://doi.org/10.1093/nar/28.12.e62
  2. Cheong, Y. H., Kim, C. Y., Chun, H. J., Moon, B. C., Park, H. C., Kim, J. K., Lee, S., Han, C., Lee, S. Y. and Cho, M. J. 2000. Molecular cloning of a soybean class III beta-1,3-glucanase gene that is regulated both developmentally and in response to pathogen infection. Plant Sci. 154:71-81 https://doi.org/10.1016/S0168-9452(00)00187-4
  3. Church, G. M. and Gilbert, W. 1984. Genomic sequencing. Proc. Natl. Acad. Sci. USA 81:1991-1995 https://doi.org/10.1073/pnas.81.7.1991
  4. Dabir, D. V., Leverich, E. P., Kim, S. K., Tsai, F. D., Hirasawa, M., Knaff, D. B. and Koehler, C. M. 2007. A role for cytochrome c and cytochrome c peroxidase in electron shuttling from Erv1. EMBO J. 26:4801-4811 https://doi.org/10.1038/sj.emboj.7601909
  5. Datson, N. A., van der Perk-de Jong, J., van den Berg, M. P., de Kloet, E. R. and Vreugdenhil, E. 1999. MicroSAGE: a modified procedure for serial analysis of gene expression in limited amounts of tissue. Nucleic Acids Res. 27:1300-1307 https://doi.org/10.1093/nar/27.5.1300
  6. De Jong, J. C., McCormack, B. J., Smirnoff, N. and Talbot, N. J. 1997. Glycerol generates turgor in rice blast. Nature 389:244-245 https://doi.org/10.1038/38418
  7. Hamer, J. E. and Talbot, N. J. 1998. Infection-related development in the rice blast fungus Magnaporthe grisea. Curr. Opin. Microbiol. 1:693-697 https://doi.org/10.1016/S1369-5274(98)80117-3
  8. Hamer, J. E., Howard, R. J., Chumley, F. G. and Valent, B. 1988. A Mechanism for Surface Attachment in Spores of a Plant Pathogenic Fungus. Science 239:288-290 https://doi.org/10.1126/science.239.4837.288
  9. International rice genome sequencing project. 2005. The mapbased sequence of the rice genome. Nature 436:793-800 https://doi.org/10.1038/nature03895
  10. Kenzelmann, M. and Muhlemann, K. 1999. Substantially enhanced cloning efficiency of SAGE (Serial Analysis of Gene Expression) by adding a heating step to the original protocol. Nucleic Acids Res. 27:917-918 https://doi.org/10.1093/nar/27.3.917
  11. Kim, S., Ahn, I. P. and Lee, Y. H. 2001. Analysis of genes expressed during rice-Magnaporthe grisea interactions. Mol. Plant-Microbe Interact. 14:1340-1346 https://doi.org/10.1094/MPMI.2001.14.11.1340
  12. Kim, S. T., Cho, K. S., Yu, S., Kim, S. G., Hong, J. C., Han, C. D., Bae, D. W., Nam, M. H. and Kang, K. Y. 2003. Proteomic analysis of differentially expressed proteins induced by rice blast fungus and elicitor in suspension-cultured rice cells. Proteomics 3:2368-2378 https://doi.org/10.1002/pmic.200300577
  13. Kim, S. T., Kim, S. G., Hwang, D. H., Kang, S. Y., Kim, H. J., Lee, B. H., Lee, J. J. and Kang, K. Y. 2004. Proteomic analysis of pathogen-responsive proteins from rice leaves induced by rice blast fungus, Magnaporthe grisea. Proteomics 4:3569-3578 https://doi.org/10.1002/pmic.200400999
  14. Kim, S. T., Yu, S., Kang, Y. H., Kim, S. G., Kim, J. Y., Kim, S. H. and Kang, K. Y. 2008a. The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Rep. 27:593-603 https://doi.org/10.1007/s00299-007-0485-6
  15. Kim, S. T., Kim, S. G., Kang, Y. H., Wang, Y., Kim, J.-Y., Yi, N., Kim, J. K., Rakwal, R., Koh, H. J. and Kang, K. Y. 2008b. Proteomics analysis of rice lesion mimic mutant (spl1) reveals tightly localized probenazole-induced protein (PBZ1) in cells undergoing programmed cell death. J. Proteome Res. 7:1750-1760 https://doi.org/10.1021/pr700878t
  16. Madden, S. L., Wang, C. J. and Landes, G. 2000. Serial analysis of gene expression: from gene discovery to target identification. Drug Discov Today 5:415-425 https://doi.org/10.1016/S1359-6446(00)01544-0
  17. Margulies, E. H., Kardia, S. L. and Innis, J. W. 2001. Identification and prevention of a GC content bias in SAGE libraries. Nucleic Acids Res. 29:E60-60 https://doi.org/10.1093/nar/29.12.e60
  18. McGee, J. D., Hamer, J. E. and Hodges, T. K. 2001. Characterization of a PR-10 pathogenesis-related gene family induced in rice during infection with Magnaporthe grisea. Mol. Plant-Microbe Interact. 14:877-886 https://doi.org/10.1094/MPMI.2001.14.7.877
  19. Munasinghe, A., Patankar, S., Cook, B. P., Madden, S. L., Martin, R. K., Kyle, D. E., Shoaibi, A., Cummings, L. M. and Wirth, D. F. 2001. Serial analysis of gene expression (SAGE) in Plasmodium falciparum: application of the technique to A-T rich genomes. Mol. Biochem. Parasitol. 113:23-34 https://doi.org/10.1016/S0166-6851(00)00378-9
  20. Peters, D. G., Kassam, A. B., Yonas, H., O'Hare, E. H., Ferrell, R. E. and Brufsky, A. M. 1999. Comprehensive transcript analysis in small quantities of mRNA by SAGE-lite. Nucleic Acids Res. 27:e39 https://doi.org/10.1093/nar/27.1.39
  21. Powell, J. 1998. Enhanced concatemer cloning-a modification to the SAGE (Serial Analysis of Gene Expression) technique. Nucleic Acids Res. 26:3445-3446 https://doi.org/10.1093/nar/26.14.3445
  22. Talbot, N. J. 2003. On the trail of a cereal killer: Exploring the biology of Magnaporthe grisea. Annu. Rev. Microbiol. 57:177-202 https://doi.org/10.1146/annurev.micro.57.030502.090957
  23. Tanaka, N., Che, F. S., Watanabe, N., Fujiwara, S., Takayama, S. and Isogai, A. 2003. Flagellin from an incompatible strain of Acidovorax avenae mediates $H_2O_2$ generation accompanying hypersensitive cell death and expression of PAL, Cht-1, and PBZ1, but not of Lox in rice. Mol. Plant-Microbe Interact. 16:422-428 https://doi.org/10.1094/MPMI.2003.16.5.422
  24. Thalmair, M., Bauw, G., Thiel, S., Dohring, T., Langebartels, C. and Sandermann, H. 1996. Ozone and ultraviolet B effects on the defense-related proteins $\beta$-1,3-glucanase and chitinase in tobacco. J. Plant Physiol. 148:222-228 https://doi.org/10.1016/S0176-1617(96)80318-X
  25. Valent, B. and Chumley, F. G. 1991. Molecular genetic analysis of the rice blast fungus, Magnaporthe grisea. Annu. Rev. Phytopathol. 29:443-457 https://doi.org/10.1146/annurev.py.29.090191.002303
  26. van den Berg, A., van der Leij, J. and Poppema, S. 1999. Serial analysis of gene expression: rapid RT-PCR analysis of unknown SAGE tags. Nucleic Acids Res. 27:e17 https://doi.org/10.1093/nar/27.17.e17
  27. Velculescu, V. E., Zhang, L., Vogelstein, B. and Kinzler, K. W. 1995. Serial analysis of gene expression. Science 270:484-487 https://doi.org/10.1126/science.270.5235.484
  28. Yamamoto, M., Wakatsuki, T., Hada, A. and Ryo, A. 2001. Use of serial analysis of gene expression (SAGE) technology. J. Immunol. Methods 250:45-66 https://doi.org/10.1016/S0022-1759(01)00305-2
  29. Zeifler, R. S., Leong, S. A. and Teeng, P. S. 1994. Rice Blast Disease. CAB International, Wallingford

Cited by

  1. Gene Expression Profiling Related to Hyphal Growth in a Temperature-Sensitive Mutant of Magnaporthe oryzae vol.12, pp.12, 2013, https://doi.org/10.1016/S2095-3119(13)60503-1
  2. In-depth insight into in vivo apoplastic secretome of rice-Magnaporthe oryzae interaction vol.78, 2013, https://doi.org/10.1016/j.jprot.2012.10.029