Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Hepatic Differentiation of Human Eyelid Adipose-Derived Stem Cells

사람의 눈지방유래 줄기세포의 간세포 분화

  • Park, Soo-Yeon (Dept. of Biotechnology, Seoul Women's University) ;
  • Park, Se-Ah (Dept. of Biotechnology, Seoul Women's University) ;
  • Kang, Hyun-Mi (Dept. of Biotechnology, Seoul Women's University) ;
  • Kim, Hae-Kwon (Dept. of Biotechnology, Seoul Women's University)
  • 박수연 (서울여자대학교 생명공학과) ;
  • 박세아 (서울여자대학교 생명공학과) ;
  • 강현미 (서울여자대학교 생명공학과) ;
  • 김해권 (서울여자대학교 생명공학과)
  • Published : 2008.09.30
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Abstract

A variety of stem cells has been emerging as therapeutic cells that can replace organ transplantation in human liver diseases. The present study focused on whether human eyelid adipose-derived stem cells (HAD) might differentiate into functional hepatocyte-like cells in vitro. HAD were isolated from human eyelid adipose tissue. Effect of dimethyl sulfoxide (DMSO), fibroblast growth factor (FGF)-2 and FGF-4 on the hepatic differentiation of HAD have been examined in vitro. Immunocytochemical analysis and PAS staining showed that HAD cultured in both DMSO and FGF-4 exhibited the most intense staining than HAD of the other experimental groups. These HAD expressed numerous hepatocyte-related genes. Immunoblotting analyses showed that HAD cultured in the presence of DMSO and FGF-4 secreted higher amount of human albumin than HAD cultured in other conditions. Urea analysis also demonstrated that these HAD produced higher amount of urea than any other groups of HAD. In conclusion, combined treatment of DMSO and FGF-4 could effectively induce the functional differentiation of HAD into hepatocyte-like cells.

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