Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of the Soybean Powder with Rich Aglycone Isoflavone on Lipid Metabolism and Antioxidative Activities in Hyperlipidemic Rats

고지혈증 흰쥐에서 비배당체 이소플라본 고함유 대두분말의 혈청 지질 대사 영향과 항산화효과

  • 임애경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 정희경 ((재)대구테크노파크 바이오산업지원센터) ;
  • 홍주헌 ((재)대구테크노파크 바이오산업지원센터) ;
  • 오정석 (신화제약연구소(주)) ;
  • 곽정훈 (신화제약연구소(주)) ;
  • 김용해 (신화제약연구소(주)) ;
  • 김대익 ((재)대구테크노파크 바이오산업지원센터)
  • Published : 2008.03.31
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Abstract

This study was performed to evaluate the effects of soybean powder with rich aglycone isoflavone through bio-transformation on lipid metabolism and antioxidative activites in diet induced hyperlipidemic rats. The rats were randomly divided into four groups: NO (basal diet normal group), CO (high fat diet control group), BFF10 (soybean powder with rich aglycone isoflavone 10% group), and BFF20 (soybean powder with rich aglycone isoflavone 20% group). After 7 weeks of BFF10 or BFF20 diets consumption, the concentrations in serum triglyceride, total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly decreased in the BFF10 and BFF20 diets groups compared with those in the CO group. The activities of alanine amino transferase and aspartate amino transferase were significantly decreased in the BFF10 and BFF20 than those in the CO group. Thiobarbituric acid reactive substance levels of serum were decreased in BFF10 and BFF20 groups compared to that of the CO group. The super oxide dismutase activites were increased in BFF10 and BFF20 groups compared to that of the CO group.

비배당체 이소플라본 고함유 대두분말이 고지방식이를 섭취한 흰쥐의 지질대사와 항산화 효과에 미치는 영향을 조사하고자 식이성 고지혈 흰쥐를 정상군(NO), 고지방식이군(CO), 고지방식이에 비배당체 이소플라본 고함유 대두분말 10%, 20%(w/w) 첨가군(BFF10, BFF20)으로 나누어 7주간 사육한 결과는 다음과 같다. 비배당체 이소플라본 고함유 대두분말 섭취는 체중과 간 무게를 유의적으로 감소시켰으며, CO군에 비해 혈청의 총 콜레스테롤, LDL-콜레스테롤, 중성지질 농도가 유의적으로 감소하였다. 간조직 손상시 활성증가가 우려되는 alanine amino transferase와 aspartate amino transferase 수준도 유의적으로 감소하였다. Thiobarbituric acid reactive substance의 생성량은 비배당체 이소플라본 고함유 대두분말의 첨가량이 높을수록 현저하게 감소되었으며 superoxide dismutase 활성은 농도 의존적으로 증가시키는 것으로 나타났다.

Keywords

References

  1. Cassidy A. 1996. Physiological effects of phytoestrogens in relation cancer and other human health risks. Proc Nutr Soc 55: 399-417 https://doi.org/10.1079/PNS19960038
  2. Messina M, Perky V, Setchell KDR, Barnes S. 1994. Soy intake and cancer risk. A review of in vitro and in vivo data. Nutr Cancer 21: 113-131 https://doi.org/10.1080/01635589409514310
  3. Anderson JW, Johnstone BM, Cook-Newell ME. 1995. Meta-analysis of the effects of soy protein intake on serum lipids. New Engl J Med 333: 276-282 https://doi.org/10.1056/NEJM199508033330502
  4. Bibgham SA, Atkinson C, Liggins J. 1998. Phytoestrogen; where are we now? British J Nutr 79: 393-406 https://doi.org/10.1079/BJN19980068
  5. Picard F, Deshaies Y, Lalonde J. 2000. Effects of the estrogen antagonist EM-652.HCl on energy balance and lipid metabolism in ovariectomized rats. Int J Obes Relat Metab Disord 24: 830-840 https://doi.org/10.1038/sj.ijo.0801240
  6. Ruiz-Larrea MB, Mohan AR, Paganga G. 1997. Antioxidant activity of phytoestrgenic isoflavones. Free Radic Res 26: 63-70 https://doi.org/10.3109/10715769709097785
  7. Wei HC, Wei LH, Frenkel K. 1993. Inhibition of tumor promotor-induced hydrogen peroxid formation in vitro and in vivo by genistein. Nutr Cancer 20: 1-12 https://doi.org/10.1080/01635589309514265
  8. Kwoon TW, Song YS, Hong JH, Moon GS, Kim JI, Hong JH. 1998. Current research on the bioactive functions of soyfoods in Korea. Korea Soybean Digest 15: 1-12
  9. Kirk EA, Sutherland P, Wang SA. 1998. Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr 128: 954-959
  10. Lee KH, Chung HK, Han JH, Sohn HS. 2003. Soy isoflavone: current usage and production. Korea Soybean Digest 20: 28-36
  11. Moon SW, Park MS, Ahn JB, Ji GE. 2003. Quality characteristics of chocolate blended with Bifidobacterium-fermented isofalvone powder. Korean J Food Sci Technol 6: 1162-1168
  12. Jeon KS, Hwang IK. 2002. The hydrolysis of isoflavones by Bifidobacterium sp. Int-57 during soymilk fermentation. Korea Soybean Digest 19: 42-47
  13. Haglund O, Loustarinen R, Wallin R, Wibell I, Saldeen T. 1991. The effect of fish oil on triglycerides, cholesterol, fibrinogen and malondialdehyde in mans supplemented with vitamin. Eur J Nutr 121: 165-172 https://doi.org/10.1093/jn/121.2.165
  14. Ohkawa H, Ohisi N, Yagi K. 1979. Assay for lipid peroxide in animal tissue by thiobabutiric acid reaction. Anal Biochem 95: 351-358 https://doi.org/10.1016/0003-2697(79)90738-3
  15. Martin JP, Dailey M, Sugarman E. 1987. Negative and positive assays of superoxide dismutase based on hematoxylin autoxidation. Arch Biochem Biophys 255: 329-336 https://doi.org/10.1016/0003-9861(87)90400-0
  16. Choi YB, Woo JG, Noh WS. 1999. Hydrolysis of ${\beta}$-glycosidic bonds of isoflavone conjugates in the lactic acid fermentation of soy milk. Korean J Food Sci Technol 31: 189-195
  17. Ju DS, Lee JG, Kim OS, Jo SY, Lee DS, Gwon JO, Choe JW. 2003. Effect of seatangle oligosaccharide drink on oxidation of serum lipid and bleeding and plasma clotting time in rats fed a hyperlipidemic diet. J Korean Soc Food Sci Nutr 32: 1364-1369 https://doi.org/10.3746/jkfn.2003.32.8.1364
  18. Mitchell GV, Jenkins MY, Grundel E. 1989. Protein efficiency ratios and net protein ratios of selected protein foods. Plant Foods Hum Nutr 39: 53-58 https://doi.org/10.1007/BF01092401
  19. Kim YH, Lee JH, Koo BK, Lee HS. 2007. Isoflavone-rich bean sprouts improves hypelipidemia. J Korean Soc Food Sci Nutr 36: 1248-1256 https://doi.org/10.3746/jkfn.2007.36.10.1248
  20. Wang H, Murphy PA. 1994. Isoflavone content in commercial soybean foods. J Agric Food Chem 42: 1666-1673 https://doi.org/10.1021/jf00044a016
  21. Choi YS, Lee SY. 1993. Cholesterol-lowering effect of soybean product (curd or crud residue) in rat. J Korean Soc Food Nutr 22: 673-677
  22. Kim MJ, Lee SS. 1995. The of dietary fiber on the lipid level and bowel function in rat. Kor J Nutr 28: 23-32
  23. Cha JY, Kim HJ, Jun BS, Cho YS. 2000. Effect of water extract of leaves from Moms alba and Cudrania tricuspidata on the lipid concentration of serum and liver in rats. Agric Chem Biotechnol 43: 303-308
  24. Myant NB. 1990. Cholesterol metabolism, LDL, and the LDL receptor. Academic Press Inc., New York. p 407
  25. Lee YN, Lee HS, Jang YA, Lee HJ, Kim BH, Kim CI. 2006. Dietary intake pattern of the Korean adult population by weight status 2001 national health and nutrition survey. Korean J Comm Nutr 11: 317-326
  26. Kim JD, Lee YI, Kim BR, Choi YS, Lee SY. 1997. Effects of Meju powder supplementation on lipid metabolism in rats fed hypercholesterolemic diet. J Korean Soc Food Sci Nutr 26: 314-318
  27. Smith EB. 1974. The relationship between plasma and tissue lipids in human atherosclerosis. Adv Lipid Res 11: 1-49
  28. Kang BH, Son HY, Ha CS, Lee HS, Song SW. 1995. Reference value of hematology and serum chemistry in Ktc: Sprague-Dawley rats. Korean J Lab Anl Sci 11: 141-145
  29. Lee JJ, Lee YM, Shin HD, Jeong YS, Lee MY. 2007. Effect of vegetable sprout power mixture on lipid metabolism in rats fed high fat diet. J Korean Soc Food Sci Nutr 36: 965-974 https://doi.org/10.3746/jkfn.2007.36.8.965
  30. Song YO, Chyun JH. 2004. Effect of ${\beta}$-carotene supplementation on lipid peroxides and antioxdative enzyme activities in hyperlipidemic rat. Korean J Nutr 37: 771-779
  31. Morel D, Chisolm GM. 1989. Antioxidative treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity. J Lipid Res 30: 1827-1834

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