Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimutagenicity and Cytotoxic Effects of Methanol Extract from Deep Sea Water Salt and Sea Tangle Added Soybean Paste (Doenjang)

해양심층수염 및 다시마분말을 첨가한 개량식 된장의 항돌연변이원성 및 암세포성장억제에 미치는 영향

  • Ham, Seung-Shi (Dept. of Biotechnology and Engineering, Kangwon National University) ;
  • Kim, Soo-Hyun (Dept. of Biotechnology and Engineering, Kangwon National University) ;
  • Yoo, Su-Jong (Dept. of Biotechnology and Engineering, Kangwon National University) ;
  • Oh, Hyun-Taek (Dept. of Biotechnology and Engineering, Kangwon National University) ;
  • Choi, Hyun-Jin (Dept. of Biotechnology and Engineering, Kangwon National University) ;
  • Chung, Mi-Ja (Dept. of Biotechnology and Engineering, Kangwon National University)
  • Published : 2008.04.30
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Abstract

This study was performed to determine the antimutagenic and anticytotoxic effects of soybean paste (doenjang) added deep sea water salt and see tangle in Salmonella Typhimurium TA98, TA100 and human cancer cell lines. In the Ames test, methanol extract of doenjang did not exhibit any mutagenicity but showed substantial inhibitory effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The methanol extracts of doenjang ($200{\mu}g$/plate) added deep sea salt and see tangle (doenjang C) showed approximately 89.1% and 70% inhibitory effect on the mutagenesis induced by MNNG and 4NQO against TA100 strain, whereas 84.4% inhibitions were observed on the mutagenesis induced by 4NQO against TA98 strain. The cytotoxic effects of doenjang methanol extracts against the cell lines with human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human gastric carcinoma (AGS), human lung carcinoma (A549) and human breast adenocarcinoma (MCF-7) were inhibited with the increase of the extract concentration. The treatment of 1.0 mg/mL doenjang C of methanol extracts showed strong cytotoxicities of 71%, 74.4%, 66.2%, 77.3%, and 71.2% against HeLa, Hep3B, AGS, A549, and MCF-7, respectively. In contrast 1 mg/mL treatment of doenjang C methanol extracts had only $10{\sim}40%$ cytotoxicity on normal human embryonal kidney cell (293). Doenjang methanol extract inhibited significantly the tumor growth in mice injected sarcoma-180 cells. Especially, doenjang C methanol extract showed an inhibition of tumor cell activity of 33% by the administration of 25 mg/kg methanol extracts.

다시마분말과 해양심층수염을 첨가하여 제조한 된장 메탄올 추출물의 항돌연변이원성과 세포독성을 측정하였다. S. Typhimurium TA98과 TA100 균주를 이용한 실험에서 모든 된장의 시료에서 돌연변이성이 없었으며, 항돌연변이 원성 실험에서는 직접변이원인 MNNG($0.4{\mu}g$/plate)의 경우 TA100 균주에서 다시마분말을 첨가한 해양심층수된장(된장C)의 시료농도 $200{\mu}g$/plate에서 89.1%의 억제효과를 나타내었으며 4NQO($0.15{\mu}g$/plate)에 대해서는 같은 시료 농도에서 70%의 억제효과를 나타내었다. 그리고 4NQO에서 TA98 균주에 대해서 다시마분말을 첨가한 해양심층수된장(된장C)은 84.4%의 높은 억제효과를 나타내었으며 모든 시료는 농도 의존적으로 억제하는 것으로 나타났다. 세포독성 효과를 알아보기 위해서 HeLa, Hep3B, AGS, A549와 MCF-7을 사용하였으며 다시마분말을 첨가한 해양심층수된장(된장C)이 1 mg/mL의 농도에서 위암세포를 제외한 자궁암세포, 간암세포, 폐암세포 및 유방암세포에서 모두 70% 이상의 높은 억제활성을 나타내었으며 그 중에서도 폐암세포에서 77.3%의 가장 높은 암세포 성장억제율을 나타내었다. 또한 다시마분말을 첨가한 해양심층수된장(된장C)은 고 형암 성장억제실험에서 대조군에 비해서 32.5%의 고형암 성장억제효과를 나타내었다.

Keywords

References

  1. Lee JS, Cheigh HS. 1997. Antioxidative characteristics of solated crude phenolics from soybean fermented foods (doenjang). J Korean Soc Food Sci Nutr 26: 376-382
  2. Cui CB, Cho MA, Jun YY, Lee DS, Ham SS. 2002. Antimutagenicity and cytotoxic effect of ethanol extract from Korean traditional mackjang added sea tangle. J East Asian Soc Dietary Life 12: 15-22
  3. Kwon SH, Shon MY. 2004. Antioxidant and anticarcinogenic effects of traditional doenjang during maturation periods. Korean J Food Preserv 11: 461-467
  4. Kim SJ, Park CW, Park SJ, Kim YS, Cho HJ, Lim DK, Kim JO, Lee JH, Ha YL. 2003. Enhanced antitumorigenicity and antimutagenicity of doenjang prepared from mushroom mycelia-cultured traditional mejus. J Korean Soc Food Sci Nutr 32: 143-148
  5. Park KY, Lee SJ, Lee KI, Rhee SH. 2005. The antitumor effect in sarcoma-180 tumor cell of mice administered with japanese apricot, garlic or ginger doenjang. Korean J Food Cookery Sci 21: 599-606
  6. Lee SL, Kim JG. 2005. Inhibitory effects of doenjang (Korean fermented soybean paste) and soybean extracts on the growth of KB cells. Kor J Env Hlth 31: 444-450
  7. Lim SY, Rhee SH, Park KY. 2004. Inhibitory effect of methanol extract of doenjang on growth and DNA synthesis of human cancer cells. J Korean Soc Food Sci Nutr 33: 936-940
  8. Rhee CH, Kim WC, Rhee IK, Lee OS, Park HD. 2006. Changes in the physicochemical property, angiotensin converting enzyme inhibitory effect and antimutagenicity during the fermentation of Korean traditional soy paste (doenjang). Korean J Food Preserv 13: 603-610
  9. Lee SJ, Lee KI, Rhee SH, Park KY. 2004. Physiological activity in doenjang added with various mushrooms. Korean J Food Cookery Sci 20: 365-370
  10. Cui CB, Lee EY, Lee DS, Ham SS. 2002. Antimutagenicity and cytotoxic effect of ethanol extract from Korean traditional doenjang added sea tangle. J Korean Soc Food Sci Nutr 31: 322-328 https://doi.org/10.3746/jkfn.2002.31.2.322
  11. Park JH, Ha AW, Cho JS. 2005. Effects of green tea soybean paste on weights and serum lipid profiles in rats fed high fat diet. Korean J Food Sci Technol 37: 806-811
  12. Colliec S, Fischer AM, Tapon-Bretaudiere J, Boisson C, Durand P, Jozefonvicz J. 1991. Anticoagulant properties of a fucoidan fraction. Thromb Res 64: 143-154 https://doi.org/10.1016/0049-3848(91)90114-C
  13. Moon DS, Kim HJ, Shin PK, Jung DH. 2005. Characteristics of chemical contents of horizontal spray salts from deep ocean water. J Kor Fish Soc 38: 65-69
  14. Maron DM, Ames BN. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  15. Scudiero DA, Shoemaker RH, Paul KD, Monks A, Tiemey S, Nofziger TH, Currens MJ, Seniff D, Boyd MR. 1988. Evaluation of a soluble tetrazolium formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res 48: 4827-4836
  16. Park KY, Moon SH, Baik HS, Cheigh HS. 1990. Antimutagenic effect of doenjang (Korean fermented soy paste) toward aflatoxin. J Korean Soc Food Nutr 19: 156-162

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