Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Degradation of Endocrine Disrupting Chemicals by Laccase Transformant of Phlebia tremellosa

아교버섯 형질전환체를 이용한 내분비장애 물질의 분해

  • Yeo, Su-Min (Department of Biochemistry, Division of Life Sciences and Research institute of Life Sciences, Kangwon National University) ;
  • Kim, Myung-Kil (Division of Wood Chemistry and Microbiology, Korea Forest Research Institute) ;
  • Choi, Hyoung T. (Department of Biochemistry, Division of Life Sciences and Research institute of Life Sciences, Kangwon National University)
  • 여수민 (강원대학교 생명과학부 생화학과.생명과학연구소) ;
  • 김명길 (국립산림과학원 화학미생물과) ;
  • 최형태 (강원대학교 생명과학부 생화학과.생명과학연구소)
  • Published : 2008.03.31
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Abstract

Endocrine disrupting chemicals (EDCs) are hard to be degraded in nature, and are also accumulated in diverse organisms. They finally give negative effects to human through the food web. White rot fungi which have lignin-degrading enzymes have high potentials for degradation of recalcitrant compounds, and a white rot fungus, Phlebia tremellosa, isolated in Korea show good degrading activity against the endocrine disrupting phthalates. We have isolated a laccase cDNA which was involved in the degradation of EDCs, and constructed a laccase expression vector to use in the genetic transformation of P. tremellosa. The expression vector was stably integrated into the chromosomal DNAs and showed increased laccase activity in transformants. One of transformants showed not only increased degradation of several EDCs but also faster estrogenic decreasing activities generated by the EDCs.

내분비장애 물질은 분해가 어렵고, 생물체에 축적되기 때문에 먹이그물을 통하여 결국 인간에게 피해를 준다. 리그닌 분해효소 군을 가진 백색부후균들은 다양한 난분해성 물질의 분해능이 우수하며, 국내에서 분리한 아교버섯은 내분비장애 물질에 속하는 프탈레이트의 분해능이 우수하다. 내분비장애 물질분해와 관련된 laccase cDNA를 발현벡터로 재조합하고 이를 형질전환 방법에 의하여 아교버섯으로 도입하였으며 도입된 발현벡터는 형질전환체의 염색체에 안정하게 존재하였다. 형질전환체들 중 가장 효소활성이 좋은 균주를 대상으로 분석한 결과 laccase 활성이 증가되었을 뿐만 아니라 내분비장애 물질의 분해능도 향상되었고, 동시에 다양한 내분비장애물질에 의한 에스트로겐 활성도 야생형 균주에 비하여 빠르게 감소시켰다.

Keywords

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