Antioxidant activity analysis of Catechin compounds in Korean green tea using HPLC On-line $ABTS^{+}$ Antioxidant screening system

HPLC On-line $ABTS^{+}$ Antioxidant screening 시스템을 이용한 한국산 녹차로부터 Catechin compounds의 황산화 활성분석

  • Lee, Kwang-Jin (Natural Product Research Center, Korea Institute of Science and Technology KIST Gangneung)
  • 이광진 (한국과학기술연구원 KIST강릉분원, 천연물소재연구센터)
  • Published : 2008.02.29

Abstract

In this work, we describes analysis of the antioxidant potential of Korean green tea phenolics using an high-performance liquid chromatography (HPLC) on-line $ABTS^{+}$ antioxidant screening method. In conjunction with the analysis of their 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS+) radical scavenging ability, the extraction of catechine compounds from Korean green tea were performed by various temperature and time. The optimum operating conditions were experimentally determined to analyze the catechine compounds in the pretreatment extracts. From the results, the extraction temperature $60^{\circ}C$, time 3 min was selected as an optimal antioxidant activity condition. The analysis by $C_{18}$ column was performed, the flow rate of mobile phase and UV wavelength was fixed at 1.0 ml/min and 254 nm, respectively. the mobile phase was composed from acetonitrile and water, and the gradient elution mode were applied.

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