Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
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NADH Variation and Process Control with NADH Fluorometer in Full Scale Biological Nutrient Removal Process

실규모의 고도처리공정에서 NADH변화 유형과 이를 활용한 공정제어

  • Kim, Han-Lae (Department of Environmental Engineering, Kyonggi University) ;
  • Cho, Jong-Bok (AquaTech, Co., Ltd.) ;
  • Cho, Il-Hyoung (Department of Environmental Engineering, Kyonggi University) ;
  • Lee, Jin-Woo (Department of Environmental Engineering, Kyonggi University) ;
  • Chang, Soon-Woong (Department of Environmental Engineering, Kyonggi University) ;
  • Lee, Si-Jin (Department of Environmental Engineering, Kyonggi University)
  • Published : 2008.12.31
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Abstract

In this study, we investigated the possibility of auto control and the proper operating factors in the BNR(Biological Nutrient Removal) process using an NADH(Reduced Nicotinamide Adenine Dinucleotide) fluorometer, which characterized the emitted fluorescence when activated by flashes of UV light at 460 nm. In terms of finding adequate operating parameters, results indicted that nitrification efficiency decreased in the controlled DO while denitrification efficiency decreased in the controlled pH. The above results indicated that controlled operating condition after combination with NADH, DO and pH was resonable. Result obtained from the correlation between NADH and pH showed that variation trend of influent loading was similar to those of NADH and pH, and also the variation cycle was repeated on a daily basis. Consequently, this result showed the increase of BOD loading caused the nitrification efficiency to decrease because air-flow, required for nitrification, was reduced, and so the NADH value was increased. From these results, it is possible to use NADH flourimetry to assess the variation of organic load and nitrification efficiency in the case of small change in influent pH such as in sewage and also to handle and operate the load variation in the auto control system using the NADH fluorometer.

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References

  1. Vaboliene, G. and Matuzevicius, A. B. : Investigationinto biologocal nutrient removal from wastewater. Journal of Environmental Engineering and Landscape Management, 8(4), 177-181, 2005
  2. Harrison, D. E. F. and Chance, B. : Fluorometric technique for monitoring changes in the level of reduced nicotinamide nucleotides in continuous culture of microorganisms. Applied and Environmental Microbiology, 19, 446-449, 1970
  3. Armiger, W. B., Lee, G., Bordacs, K. and Tracy, K. D. : Automated on-line control strategy for the optimization of nutrient removal processes, Proceedings of the Fifth IAWQ Workshop on Instrumentation, Control and Automation of Water and Wastewater Treatment and Transport System, Japan, 26 July, 1990
  4. Teusink, B. : Exposinga complex metabolic system : glycolysis in saccharomyces cerevisiae, Ph.D. thesis, University of Amsterdam, Amsterdam, 1990
  5. Yang, X., Lee, J. F., Maneshin, S. K. and Kolb, M. E. : Apparatus and method for monitoring and controlling biological activity in wastewater and controlling the treatment thereof, US Patent 5552319, 1996
  6. Mah, T. J. : Use of culture fluorescence monitoring for control and optimization of biological nutrient removal form wastewaters. Ph.D. thesis, University of British columbia, Canada, 1995
  7. Chance, B. and Williams, G. R. : The respiratory chain and oxidative phosphory-lation. Advanced Enzymnology, 17, 65-134, 1956
  8. Trivedi, H. and Heinen, N. : Simultaneous nitrification/denitrification by monitoring NADH fluorescence in activated sludge, In: WEFTEC 2000, Annual Conference and Exposition on Water Quality and Wastewater Treatment, 73rd, Anaheim, CA, United States, 2262-2278, 2000
  9. Trivedi, H. and Tim, W. : Industrial Waste- water, Water Environment Federation, 5-7, 2002
  10. Rittman, B. E. and Langeland, W. E. : Simultaneous denitrification with nitrification in single-channel oxidation ditches. Journal of Water Pollution Control Federation, 57, 300-308, 1985
  11. Van Huyssteen, J. A., Barnard, J. L. and Hendriksz, J. : The olifantsfontein nutrient removal plant. Water Science and Technology, 22, 1-8, 1990
  12. Srivastava, S., Harsh, S. and Srivastava, A. K. : Use of NADH fluorescence measurement for on-line biomass estimation and characterization of metabolic status in bioreactor cultivation of plant cells for azadirachtin (a biopesticide) production. Process Biochemistry xxx, xxx-xxx, 2008
  13. Huang, L. and Ju, L. K. : Sludge settling and online NAD(P)H fluorescence profiles in wastewater treatment bioreactors operated at low dissolved oxygen concentrations. Water Research, 41, 1877-1884, 2007 https://doi.org/10.1016/j.watres.2007.01.040
  14. Helmo, K. and Heinen, N. : Novel method for the control of biodegradation, EP No. 0 828 692 B1, 1996
  15. Ju, L. K. and Nallagatla Prakash, B. : Monitoring and kinetic study of ammonia oxidation using dissolved oxygen electrode and NAD(P)H fluorometer. Water Research, 37, 188-196, 2003 https://doi.org/10.1016/S0043-1354(02)00242-7
  16. Kim, Y. G. and Cho, I . H. : A s tudy on the removal of nitrogen and phosphorus of municipal wastewater with biological coated media. Journal of Environmental Health Sciences, 32(1), 27-35, 2006
  17. Lee, W. I., Lee, J. M., Kim, K. D., Cho, G. W., Choi, J. H. and Chung, T. H. : The Analysis of NADH Fluorescence Signal in the Biological Nitrogen Removal. 2005 Academic Conference, Korean Society on Water Quality. Korean Society of Water and Wastewater, 223-228, 2005
  18. Melissa Wos and Peter Pollard : Sensitive and meaningful measures of bacterial metabolic activity using NADH fluorescence. Water Research, 40, 2084-2092, 2006 https://doi.org/10.1016/j.watres.2006.03.020
  19. Lee, W. I., Lee, J. M., Kim, Y. J. and Chung, T. H. : Characteristic of NADH Fluorescence Sensor for Online Aeration Control of Biological Nitrogen Removal Process. 2006 Academic Conference, Korean Society on Water Quality. Korean Society of Water and Wastewater, 67-72, 2006
  20. Oh, K. K. : A Study on the Simultaneous Nitrification and Denitrification with NADH Sensor in Oxdiation Ditch Process, master's course thesis, Ajou university, 2006
  21. Chen, F., Xia, Q. and Ju, L. K. : Aerobic denitrification of pseudomonas aeruginosa monitored by online NAD(P)H fluorescence. Applied and Environmental Microbiology, 69(11), 6715-6722, 2003 https://doi.org/10.1128/AEM.69.11.6715-6722.2003