Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
권호 표지
DOI QR코드

DOI QR Code

Polymorphism Analysis and Expression of the CCAAT/Enhancer Binding Protein β(C/EBPβ) in the Korean Native Cattle and Black Cattle Storage

한우와 흑한우 CCAAT/Enhancer Binding Protein β(C/EBPβ) 유전자의 발현과 다형분석

  • Kim, Hey-Min (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Lee, Sang-Mi (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Pack, Hyo-Young (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Yoon, Seul-Ki (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Yoon, Du-Hak (National Institute of Animal Science) ;
  • Lee, Seung-Soo (National Institute of Subtropical Agriculture) ;
  • Ko, Moon-Suck (National Institute of Subtropical Agriculture) ;
  • Moon, Seung-Ju (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Kang, Man-Jong (Department of Animal Science, Insti. of Ag. Sci. and Tech., College of Agriculture & Life Science, Chonnam National University)
  • 김혜민 (전남대학교 동물자원학부 농업과학기술연구소) ;
  • 이상미 (전남대학교 동물자원학부 농업과학기술연구소) ;
  • 박효영 (전남대학교 동물자원학부 농업과학기술연구소) ;
  • 윤슬기 (전남대학교 동물자원학부 농업과학기술연구소) ;
  • 윤두학 (축산과학원) ;
  • 이성수 (난지농업연구소) ;
  • 고문석 (난지농업연구소) ;
  • 문승주 (전남대학교 동물자원학부 농업과학기술연구소) ;
  • 강만종 (전남대학교 동물자원학부 농업과학기술연구소)
  • Published : 2008.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

The CCAAT/enhancer binding protein β(C/EBPβ), a member of the leucine zipper DNA-binding protein of transcription factor, plays a crucial role in the control of early phases of adipocyte differentiation. In this studies, we report the identification, characterization, and expression of the Korean native cattle C/EBPβ gene. The Korean native cattle and black cattle C/EBPβ cDNA includes a 1047bp open reading frame encoding a protein of 348 amino acids. The C/EBPβ cDNA sequence of the Korean native cattle and black cattle shows high conservation with the corresponding amino acid sequences reported in other species. The distribution of C/EBPβ mRNA in various tissues of Korean native cattle aged 26 months was investigated using Northern Blot analysis. The C/EBPβ expression was detected in adipose tissue, lung, sirloin while expression was not detected in heart, kidney, small intestine, colon, and liver. However, we are analyzed polymorphism of bZIP domain in the C/EBPβ gene. A polymorphism was not identified at this position.

지방세포분화 초기에 중요한 역할을 수행하는 것으로 보고되고 있는 C/EBPβ 유전자를 cloning하기 위하여 한우 26개월령 등심조직을 이용하여 Total RNA를 추출하고 RT-PCR을 수행한 결과 한우 및 흑한우 C/EBPβ는 1047bp, 348 아미노산 서열을 가지고 있었다. 한우 및 흑한우 C/EBPβ의 아미노산 서열을 NCBI에 등록된 Japanese black cattle C/EBPβ(NCBI accession No. NM_176788) 비교한 결과 약 97%의 상동성을 나타내었다. 또한 인간, 닭, 생쥐, 쥐의 C/EBPβ 아미노산 서열을 비교한 결과 각각 96%, 58%, 74%, 75%의 상동성을 나타내었다. 그러나 각 종간의 leucine zipper domain간의 상동성은 매우 높게 나타났다. 한우 C/EBPβ의 발현은 폐, 등심, 지방조직에서 나타나고 있으나, 지방조직에서 좀 더 많은 발현을 보이고 있었다. 그리고 C/EBPβ 유전자의 bZIP 영역에서 polymorphism이 나타나지 않음을 확인하였다.

Keywords

References

  1. Brun, R. P., Kim, J. B., Hu, E., Altiok, S. and Spiegelman, B. M. 1996. Adipocyte differentiation: a transcriptional regulatory cascade. Curr Opin Cell Biol. 8:826-832 https://doi.org/10.1016/S0955-0674(96)80084-6
  2. Cao, Z., Umek, R. M. and McKnight, S. L. 1991. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes & Dev. 5:1538-1552 https://doi.org/10.1101/gad.5.9.1538
  3. Christy, R. J., Kaestner, K. H., Geiman, D. E. and Lane, M. D. 1991. CCAAT/Enhancer binding protein gene promoter: Binding of nuclear factors during differentiation of 3T3-L1 preadipocytes. Proc. Natl. Acad. Sci. USA 88:2593-2597 https://doi.org/10.1073/pnas.88.6.2593
  4. Cianzio, D. S., Topel, D. G., Whitehurst, G. B., Beitz, D. C. and Self, H. L. 1985. Adipose tissue growth and cellularity: changes in bovine adipocyte size and number. J Anim Sci. 60:970-976 https://doi.org/10.2527/jas1985.604970x
  5. Eleswarapu, S. and Jiang, H. 2005. Growth hormone regulates the expression of hepatocyte nuclear factor-3 gamma and other liver-enriched transcription factors in the bovine liver. J. Endocrinol. 184:95-105 https://doi.org/10.1677/joe.1.05821
  6. Hamm, J. K., Park, H. P. and Farmer, S. R. 2001. A role for C/EBP$\beta$ in regulating per- oxisome proliferator-activated receptor ${\gamma}$ activity during adipogenesis in 3T3-L1 preadipocytes. J. Biol. Chem. 276:18464-18471 https://doi.org/10.1074/jbc.M100797200
  7. Harper, G. S. and Pethick, D. W. 2004. How might marbling begin? Australian Journal of Experimental Agriculture. 44:653-662 https://doi.org/10.1071/EA02114
  8. Jeoung, Y. H. and Lee, S. M. 2004. Molecular cloning and mRNA expression of the bovine peroxisome proliferator-activated receptor gamma(PPAR${\gamma}$). J. Anim. Sci. & Technol.(Kor.) 46:23-30 https://doi.org/10.5187/JAST.2004.46.1.023
  9. Lekstrom-Himes, J. and Xanthopoulos, K. G. 1998. Biological role of the CCAAT/Enhancer- binding protein family of transcription factors. J. Biol. Chem. 273:285450-285458
  10. Loftus, T. M. and Lane, M. D. 1997. Modulating the transcriptional control of adipogenesis. Curr Opin Genet Dev. 7:603-608 https://doi.org/10.1016/S0959-437X(97)80006-8
  11. Mandrup, S. and Lane, M. D. 1997. Regulating Adipogenesis. J. Biol. Chem. 272:5367-5370 https://doi.org/10.1074/jbc.272.9.5367
  12. Park, B. H., Qiang, L. and Farmer, S. R. 2004. Phosphorylation of C/EBP$\beta$ at a consensus extracellular signal-regulated kinase/glycogen synthase kinase 3 site is required for the induction of adiponectin gene expression during the differentiation of mouse fibroblasts into adipocytes. Mol. Cell. Biol. 24:8671-8680 https://doi.org/10.1128/MCB.24.19.8671-8680.2004
  13. Grimaldi, P. A. 2001. The roles of PPARs in adipocyte differentiation. Progress in Lipid Research. 40:269-281 https://doi.org/10.1016/S0163-7827(01)00005-4
  14. Tang, Q. -Q., Jiang, M.-S. and Lane, M. D. 1999. Repressive effect of Sp1 on the C/EBP${\alpha}$ gene promoter: Role in adipocyte differentiation. Mol. Cell. Biol. 19:4855-4865 https://doi.org/10.1128/MCB.19.7.4855
  15. Tang, Q. -Q., Otto, T. C. and Lane, M. D. 2003. CCAAT/enhancer-binding protein $\beta$ is required for mitotic clonal expansion during adipogenesis. Proc Natl Acad Sci. U.S.A. 100:850-855 https://doi.org/10.1073/pnas.0337434100
  16. Thomassin, H., Hamel, D., Bernier, D., Guertin, M. and Belanger, L. 1992. Molecular cloning of two C/EBP-related proteins that bind to the promoter and the enhancer of the ${\alpha}$1-fetoprotein gene. Further analysis of C/EBP$\beta$ and C/EBP$\gamma$. Nucleic Acids Res. 20:3091-3098 https://doi.org/10.1093/nar/20.12.3091
  17. Yamaoka, I., Taniguchi, Y. and Sasaki, Y. 1997. Rapid communication: nucleotide sequence of bovine C/EBP beta gene. J. Anim. Sci. 75:587
  18. Zhang, J. W., Tang, Q. Q., Vinson, C. and Lane, M. D. 2004. Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes. Proc Natl Acad Sci. U.S.A. 101:43-47 https://doi.org/10.1073/pnas.0307229101