Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Dietary Protein Level and Supplementation of Conjugated Linoleic Acid on Growth Performance and Meat Quality Parameters in Finishing Pigs

사료 중 단백질 수준 및 CLA(Conjugated Linoleic Acid) 첨가가 비육돈의 생산성 및 육질특성에 미치는 영향

  • Moon, Hong-Kil (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Lee, Sung-Dae (Swine Science Division, National Institute of Animal Science, RDA, Cheonan 330-801, Korea) ;
  • Jung, Hyun-Jung (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Young-Hwa (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Park, Jun-Cheol (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Ji, Sang-Yun (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, Chong-Dae (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kwon, Oh-Sub (Swine Science Division, National Institute of Animal Science, RDA) ;
  • Kim, In-Cheul (Swine Science Division, National Institute of Animal Science, RDA)
  • 문홍길 (농촌진흥청 축산과학원 양돈과) ;
  • 이성대 (농촌진흥청 축산과학원 양돈과) ;
  • 정현정 (농촌진흥청 축산과학원 양돈과) ;
  • 김영화 (농촌진흥청 축산과학원 양돈과) ;
  • 박준철 (농촌진흥청 축산과학원 양돈과) ;
  • 지상윤 (농촌진흥청 축산과학원 양돈과) ;
  • 김종대 (농촌진흥청 축산과학원 양돈과) ;
  • 권오섭 (농촌진흥청 축산과학원 양돈과) ;
  • 김인철 (농촌진흥청 축산과학원 양돈과)
  • Published : 2008.10.01
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Abstract

This study was conducted to investigate effects of dietary crude protein(CP) level and supplementation of conjugated linoleic acid(CLA) on growth performances and meat quality parameters in finishing pigs. The experiment was designed using protein levels(11.3%, 16.0%) and CLA levels(0%, 2.5%) according to 2×2 factorial design. A total of forty-eight pigs [(Landrace×Yorkshire)×Duroc] with an average initial weight of 79±1kg were allotted to one of four dietary treatments. Each treatment had four replications of three pigs per replicate. Final body weight(P<0.05) and average daily gain(P<0.01) were lower in 11.3% CP treatments than in 16.0% CP treatments, while feed/gain was high(P<0.01) in 11.3% CP treatments compared with 16.0% CP treatments. Carcass weight was lighter in 11.3% CP treatments(P<0.001) and CLA 2.5% treatments(P<0.01) than in 16.0% CP and CLA 0% treatments, respectively. A significant interaction between CP and CLA on carcass weight was observed(P<0.01), where supplementation of 2.5% CLA to finishing diets decreased carcass weight in 16.0% CP treatments, while no difference was found in 11.3% CP treatments. Backfat thickness was thinner in 11.3% CP treatments and CLA 2.5% treatments than in 16.0% CP and CLA 0% treatments, respectively(P<0.05). A significant interaction between CP and CLA on backfat thickness was observed(P<0.001), where supplementation of 2.5% CLA to finishing diets decreased backfat thickness in 16.0% CP treatments, while no difference was found in 11.3% CP treatments. Marbling score and intramuscular fat contents were higher in 11.3% CP treatments than in 16.0% CP treatments(P<0.01). In conclusion, feeding of protein-deficient diets in finishing pigs could produce favorable pork with high marbling score and thinner backfat. On the other hand, supplementation of CLA was considered to decrease backfat thickness when diets with optimal level of crude protein were fed to finishing pigs.

본 연구에서는 비육돈 사료내 조단백질 및 CLA 수준과 그들간의 상호작용이 성장 및 육질특성에 미치는 효과를 구명하기 위하여 수행하였다. 공시동물은 일령이 비슷하고 체중이 79±1kg되는 3원교잡종 48두를 공시하였다. 처리구는 시료 중 조단백질 함량 2수준(11.3%, 16.0%)과 CLA 함량 2수준(0%, 2.5%)의 2×2 요인실험으로, 처리당 4반복, 반복당 3두씩 완전임의 배치하였다. 종료체중(P<0.05)과 일당증체량(P<0.01)은 조단백질 11.3% 급여구가 16.0% 급여구에 비해 유의적으로 낮았으며, 사료요구율(feed/gain, P< 0.01)은 조단백질 11.3% 급여구가 16.0% 급여구에 비해 유의적으로 높았다. 도체중량은 조단백질 11.3% 급여구가 16.0% 급여구에 비해(P<0.001), CLA 2.5% 첨가구가 CLA 무첨가구에 비해(P<0.01) 유의적으로 낮았다. 조단백질 11.3% 첨가구에서는 CLA의 첨가가 도체중량에 영향을 미치지 않았으나, 조단백질 16.0% 첨가구에서는 CLA 2.5% 첨가 시 도체중량이 유의적으로 감소하여, 조단백질과 CLA간 상호작용이 나타났다(P<0.01). 등지방두께는 조단백질 11.3% 급여구가 16.0% 급여구에 비해, CLA 2.5% 첨가구가 CLA 무첨가구에 비해 유의적으로 낮았다(P<0.05). 조단백질 11.3% 첨가구에서는 CLA의 첨가가 등지방두께에 영향을 미치지 않았으나, 조단백질 16.0% 첨가구에서는 CLA 2.5% 첨가 시 등지방두께가 유의적으로 감소하여, 조단백질과 CLA간 상호작용이 나타났다(P<0.001). 상강도(marbling score) 및 근내지방(intramuscular fat) 함량은 조단백질 11.3% 수준의 사료를 섭취한 시험구가 조단백질 16.0% 수준의 사료를 섭취한 시험구보다 유의적으로 높았다(P<0.01). 결론적으로 비육후기에 저 단백질 사료 급여로 등지방은 얇으면서 기호성이 높은 고 상강도의 돈육생산이 가능할 것으로 사료된다. 한편 정상적인 수준의 조단백질 사료 급여 시 CLA의 첨가로 등지방두께를 낮출 수 있을 것으로 사료된다.

Keywords

References

  1. AOAC. 1995. Official method of analysis, 15th edition. Association of Official Analytical Chemist, Washington. DC
  2. Blanchard, P. J., Ellis, M., Warkup, C. C., Hardy, B., Chadwick, J. P. and Deans, G. A. 1999. The influence of rate of lean and subcutaneous fat tissue development on pork eating quality. Anim. Sci. 68:477-485 https://doi.org/10.1017/S1357729800050487
  3. Brown, J. M. and McIntosh, M. K. 2003. Conjugated linoleic acid in humans: Regulation of adiposity and insulin sensitivity. J. Nutr. 133:3041-3046
  4. Castell, A. G., Cliplef, R. L., Poste-Flyun, L. M. and Butler, G. 1994. Performance, carcass and pork characteristics of castrates and gilts self-fed diets differing in protein content and lysine: energy ratio. Can. J. Anim. Sci. 74:519-528 https://doi.org/10.4141/cjas94-073
  5. Cisneros, F., Ellis, M., Baker, D. H., Easter, R. A. and Mekeith, F. K. 1996. The influence of short-term feeding of amino acid-deficient diets and high dietary leucine levels on the intramuscular fat content of pig muscle. Anim. Sci. 63:517-522 https://doi.org/10.1017/S1357729800015411
  6. Corino, C., Magni, S., Pastorelli, G., Rossi, R. and Mourot, J. 2001. CLA in heavy pig nutrition: influence on growth, meat quality and sensory characteristics of dry-cured ham. J. Anim. Sci. 78(Suppl. 1):195
  7. Corino, C., Musella, M., Pastorelli, G., Rossi, R., Paolone, K., Costanza, L., Manchisi, A. Maiorano, G. 2008. Influences of dietary conjugated linoleic acid(CLA) and total lysine content on growth, carcass characteristics and meat quality of heavy pigs. Meat Sci. 79:307-316 https://doi.org/10.1016/j.meatsci.2007.10.001
  8. Donato, J., Pedrosa, R. G., Cruzat, V. F., Pires, I. S. O. and Tirapegui, J. 2006. Effects of leucine supplementation on the body composition and protein status of rats submitted to food restriction. Nutrition 22:520-527 https://doi.org/10.1016/j.nut.2005.12.008
  9. Dugan, M., Aarhus, J. L., Jeremiah, L. E., Kramer, J. K. G. and Schaefer, A. L. 1999. The effects of feeding conjugated linoleic acid on subsequent pork quality. Can. J. Anim. Sci. 79:45-51 https://doi.org/10.4141/A98-070
  10. Essen-Gustavsson, B., Karlsson, A., Lundstom, K. and Enfalt, A. C. 1994. Intramuscular fat and muscle fibre lipid contents in halothane-gene-free pigs fed high or low protein diets and its relation to meat quality. Meat Sci. 38:269-277 https://doi.org/10.1016/0309-1740(94)90116-3
  11. Evans, M., Geigerman, C., Cook, J., Curtis, L., Kuebler, B. and McIntosh, M. 2000. Conjugated linoleic acid suppresses triglyceride accumulation and induces apoptosis in 3T3-L1 preadipocytes. Lipids. 35:899-910 https://doi.org/10.1007/S11745-000-0599-6
  12. Fitch, H. B. 1996. Conjugated linoleic acid offers research promise. INFORM. 7:152-159
  13. Gatlin, L. A., See, M. T., Larick, D. K., Lin, X. and Odle, J. 2002. Conjugated linoleic acid combination with supplemental dietary fat alters pork fat quality. J. Nutr. 132:3105-3112
  14. Goerl, K. F., Eilert, S. J., Mandigo, R. W., Chen, H. Y. and Miller, P. S. 1995. Pork characteristics as affected by two populations of swine and six crude protein levels. J. Anim. Sci. 73:3621-3626 https://doi.org/10.2527/1995.73123621x
  15. Ha, Y. L., Park, G. B., Kang, S. J., Shim, K. H. and Kim, J. O. 1998. Newly recongnized multifunctional fatty acids for the production of high quality meat, fish and agricultural products. Ministry of Agriculture and Forestry (Report):1-314
  16. Ha., Y. L., Storkson, J. M. and Pariza, M. W. 1990. Inhibitation of benzo(a)pyrene-induced mouse forestomach neoplasis by conjugated dienoic derivatives of linoleic acid. Cancer Res. 50:1097-1101
  17. Heo, K., Odle, J., Han, I. K., Cho, W., Seo, S., van Heugten, E. and Pilkington, D. H. 2000. Dietary L-carnitine improves nitrogen utilization in growing pigs fed low energy, fat-containing diets. J. Nutr. 130:1809-1814
  18. Hodgson, R. R., Davis, G. W., Smith, G. C., Savell, J. W. and Cross, H. R. 1991. Relationships between pork loin palatability traits and physical characteristics of cooked chops. J. Anim. Sci. 69:4858-4865 https://doi.org/10.2527/1991.69124858x
  19. Joo, S. T., Lee, J. I., Ha, Y. L. and Park, G. B. 2002. Effects of dietary conjugated linoleic acid on fatty acid composition, lipid oxidation, color, and water-holding capacity of pork loin. J. Anim. Sci. 80:108-112
  20. Karlsson, A., Enfalt, A. C., Essen-Gustavsson, B., Lundstrom, K., Rydhmer, L. and Stern, S. 1993. Muscle histochemical and biochemical properties in relation to meat quality during selection for increased lean tissue growth rate in pigs. J. Anim. Sci. 71:930-938
  21. Katsumata, M., Kobayashi, S., Matsumoto, M., Tsuneishi, E. and Kaji, Y. 2005. Reduced intake of dietary lysine promotes accumulation of intramuscular fat in the Longissimus dorsi muscles of finishing gilts. Anim. Sci. J. 76:237-244 https://doi.org/10.1111/j.1740-0929.2005.00261.x
  22. Kerr, B. J., McKeith, F. K. and Easter, R. A. 1995. Effect on performance and carcass charac- teristics of nursery to finisher pigs fed reduced crude protein, amino acid supplemented diets. J. Anim. Sci. 73:433-440
  23. Kim, J. H., Park, B. Y., Yoo, Y. M., Cho, S. H., Kim, Y. K., Lee, J. M., Yun, H. J. and Kim, K. N. 2002. Characterstics of carcass and meat yields of fattening pigs by production step. J. Anim. Sci. & Technol. (Kor.). 44:793-800 https://doi.org/10.5187/JAST.2002.44.6.793
  24. Lauridsen, C., Mu, H. and Henckel, P. 2005. Influence of dietary conjugated linoleic acid (CLA) and age at slaughtering on performance, slaughter- and meat quality, lipoproteins, and tissue deposition of CLA in barrows. Meat Sci. 69:393-399 https://doi.org/10.1016/j.meatsci.2004.08.009
  25. Loughmiller, J. A., Nelssen, J. L., Goodband, R. D., Tokach, M. D., Titgemeyer, E. C. and Kim, I. H. 1998. Influence of dietary lysine on growth performance and carcass characteristics of late-finishing gilts. J. Anim. Sci. 76:1075-1080
  26. Martin, D., Muriel, E., Gonzalez, E., Viguera, J. and Ruiz, J. 2008. Effect of dietary conjugated linoleic acid and monounsaturated fatty acids on productive, carcass and meat quality traits of pigs. Livest. Sci. (in press)
  27. Mayes, P. A. 1996. Oxidation of fatty acids: ketogenesis. In: Murray, R. K., Granner, D. K., Mayes, P. A. and Rodwell, V. W.(eds), Harper's Biochemistry, 24th edition, Appleton and Lange, Stanford. pp:224-235
  28. Mueller, H. L., Kirchgessner, M., Roth, F. X. and Stangl, G. I. 2000. Effect of conjugated linoleic acid on energy metabolism in growing-finishing pigs. J. Ani. Phys. Ani. Nutrition. 83:85-94 https://doi.org/10.1046/j.1439-0396.2000.00253.x
  29. NRC. 1998. Nutrient requirement of swine, 10th edition. National Academy Press, Washington, DC
  30. Pariza, M. W., Park, Y. and Cook, M. E. 2000. Mechanisms of action of conjugated linoleic acid: Evidence and speculation. Pro. Soc. Exp. Biol. Med. 223:8-13
  31. Park, B. Y., Yoo, Y. M., Kim, J. H., Cho, S. H., Kim, S. T., Lee, J. M. and Kim, Y. K. 1999. Effect of intramuscular fat contents on meat quality of pork loins. Kor. J. Anim. Sci. 41:59-64
  32. Park, J. C., Kim, Y. H., Jung, H. J., Lee, S. D., Cho, K. H., Kim, I. C., Lee, S. J. and Moon, H. K. 2006. Effects of Dietary L-leucine levels in low-lysine diets on growth performance and meat quality parameters in finishing duroc pigs. J. Anim. Sci. & Technol. (Kor.). 48:813-818 https://doi.org/10.5187/JAST.2006.48.6.813
  33. Park, Y., Albreight, K. J., Liu, W., Storkson, J. M., Cook, M. E. and Pariza, M. W. 1997. Effect of conjugated linoleic acid on body composition in mice. Lipids 32:853-858 https://doi.org/10.1007/s11745-997-0109-x
  34. Pastorelli, G., Moretti, V., Macchioni, P., Lo Fiego, D., Santoro, P., Panseri, S., Rossi, R. and Corino, C. 2005. Influence of dietary conjugated linoleic acid on the fatty acid composition and volatile compounds profile of heavy pig loin. J. Sci. Food Agric. 85:2227-2234 https://doi.org/10.1002/jsfa.2241
  35. Ramsay, T. G., Evock-Clover, C. M., Steele, N. C. and Azain, M. J. 2001. Dietary conjugated linoleic acid alters fatty acid composition of pig skeletal muscle and fat. J. Anim. Sci. 79:2152-2161
  36. SAS. 1998. Applied statistics and the SAS programming language. SAS Institute INC, Cary, NC, USA
  37. Stahly, T. S., Cromwell, G. L. and Aviotti, M. P. 1979. The effect of environmental temperature and dietary lysine source and level on the performance and carcass characteristics of growing swine. J. Anim. Sci. 49:1242-1251 https://doi.org/10.2527/jas1979.4951242x
  38. Sun, D., Zhu, X., Qiao, S. and Li, D. 2004. Effects of conjugated linoleic acid levels and feeding intervals on performance, carcass traits and fatty acid composition of finishing barrows. Arch. Anim. Nutr. 58:277-286 https://doi.org/10.1080/00039420412331273286
  39. Tischendorf, F., Shone, F., Kircheim, U. and Jahreis, G. 2002. Influence of a conjugated linoleic acid mixture on growth, organ weights, carcass traits and meat quality in growing pigs. J. Anim. Physiol. Anim. Nutr. 86:117-128 https://doi.org/10.1046/j.1439-0396.2002.00366.x
  40. Wiegand, B. R., Sparks, J. C., Parrish, Jr. F. C. and Zimmerman, D. R. 2002. Duration of feeding conjugated linoleic acid influences growth performances, carcass traits and meat quality of finishing barrows. J. Anim. Sci. 80:637-643
  41. Witte, D. P., Ellis, M., McKeith, F. K. and Wilson, E. R. 2000. Effect of dietary lysine level and environmental temperature during the finishing phase on the intramuscular fat content of pork. J. Anim. Sci. 78:1272-1276

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