Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Discovery of Candidate SNP Related to Meat Quality Using the BcSNPdb and Cattle QTLdb in Hanwoo

한우에서 Cattle QTLdb와 BcSNPdb를 이용한 육질연관 후보 SNP 발굴

  • Kim, Dae-Hyun (Department of Animal Resources and Biotechnology Graduate School Yeungnam University) ;
  • Lee, Yoon-Seok (Department of Animal Resources and Biotechnology Graduate School Yeungnam University) ;
  • Oh, Young-Sook (Institute of Biotechnology Yeungnam University) ;
  • Choi, Chang-Bon (School of Biotechnology Yeungnam University) ;
  • Yeo, Jung-Sou (School of Biotechnology Yeungnam University)
  • 김대현 (영남대학교 대학원 동물생명과학과) ;
  • 이윤석 (영남대학교 대학원 동물생명과학과) ;
  • 오영숙 (영남대학교 생명공학연구소) ;
  • 최창본 (영남대학교 생명공학부) ;
  • 여정수 (영남대학교 생명공학부)
  • Published : 2008.12.31
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Abstract

This study was aimed to find SNPs related with marbling score in Hanwoo(Korean cattle) using BcSNPdb. This study was searched QTL region related with marbling score and extracted 3,605 SNPs by applying BcSNPdb. Among these SNPs, 347 nonsynonymous SNP were selected and 160 SNPs were verified by PCR and finally proven with application to experimental data of the national progeny test. BTS_003888, BTS_012665 and BTS_009454 candidate SNPs were revealed significantly associated with marbling score(P<0.05), and BTS_025951 candidate SNPs was significantly associated with backfat thickness(P<0.05). From the result, SNPs from BTS_003888, BTS_009454, BTS_052584 and BTS_025951 were considered to be useful for the advancement in selective improved model in marbling score and backfat thickness of Hanwoo.

본 연구는 한우의 근내지방도와 연관된 SNP를 규명하고자 Cattle QTLdb와 BcSNPdb를 이용하였다. 먼저 Cattle QTLdb에서 근내지방도에 관련된 QTL 영역을 탐색한 후 BcSNPdb에 적용하여 총 3605개의 SNP를 발굴하였고 이 중에서 347개의 nonsynonymous SNP를 선정하여 PCR 반응이 확인된 160개의 SNP를 확인하였다. 확인된 160개의 SNP를 30차에서 35차 국가후대검정우(n=476)에서 혈연관계가 없으며 근내지방도 성적을 근거로 정규분포상에서 평균±2SD 범위 밖의 성적이 높은 집단 50두와 낮은 집단 50두에 적용한 결과 6개의 근내지방도 관련 SNP(BTS_003888, BTS_012665, BTS_ 018201, BTS_025951, BTS_009454, BTS_052584)를 확인하였다. 확인된 6개의 SNP를 30차에서 35차 국가후대검정우 집단(n=476)에 적용한 결과 BTS_003888, BTS_009454, BTS_052584의 SNP에서 근내지방도와 유의적인 차이를 나타내었고 BTS_025951의 SNP에서는 등지방두께와 유의적인 차이를 보였다(P<0.05). 따라서 본 연구의 결과에서 밝혀진 BTS_003888, BTS_ 009454, BTS_052584, BTS_025951 SNP들은 한우의 근내지방도와 등지방두께의 선발개량모형에 포함하여 진일보하는 보탬의 기틀이기를 기대한다.

Keywords

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