The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Isolation and Identification of Micromonospora sp. Showing Nematocidal Activity Against Pine Wood Nematode

소나무재선충에 대한 살선충 활성을 나타내는 Micromonospora sp.의 분리 및 동정

  • Park, Dong-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jae-Chan (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Pan-Kyung (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Jin (Functional Metabolomics Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • 박동진 (한국생명공학연구원 기능대사물질연구센터) ;
  • 이재찬 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김판경 (한국생명공학연구원 기능대사물질연구센터) ;
  • 김창진 (한국생명공학연구원 기능대사물질연구센터)
  • Published : 2008.03.31
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Abstract

For the isolation of Actinomycetes showing nematocidal activity against Pine wood nematode, Bursaphelenchus xylophilus, about 2000 culture broth of Actinomycetes were tested and their activity were compared with that of Streptomyces avermitilis resulting a selected strain AW050027. The cultural, morphological and physiological analysis was performed for the identification of a selected strain. Phylogenetic analyses based on 165 rDNA gene sequences showed that the selected strain AW050027 belonged to the genus Micromonospora and M. corioriae $NAR01^T$ was the closest neighbors, sharing 98.9% 165 rDNA gene sequence similarity.

소나무재선충에 대한 살선충 활성을 나타내는 방선균의 탐색 및 동정을 위하여 2000여개의 방선균배양액 라이브러리로부터 살선충 활성을 탐색하였다. 현재 소나무재선충에 대한 생물학적 방제제로 사용되고 있는 Streptomyces avermitilis 균주 배양액의 살선충 활성과 비교하여 선발한 결과, 강력한 살선충 활성을 나타내는 균주 AW050027을 균주를 최종 선발하였다. 선발군주에 대하여 형태학적, 배양학적, 생리학적 특성 분석 및 분자계통분류학적 분석을 한 결과 선발군주는 Micromonospora coriariae $NAR01^T$ 균주와 98.9%의 상동성을 가진 균주로 동정되었다.

Keywords

References

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