Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Control of MPF Activity and Nuclear Remodeling of Somatic Cell Nuclear Transfer Bovine Embryos by Chemical Treatments

소 체세포 핵이식란의 화학적 처리에 의한 MPF 활성 및 핵의 Remodeling 조절

  • Choi, Yong-Lak (School of Veterinary Medicine, Kangwon National University) ;
  • Lee, Yu-Mi (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Ho-Jeong (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Joo-Hee (School of Veterinary Medicine, Kangwon National University) ;
  • Kwon, Dae-Jin (School of Veterinary Medicine, Kangwon National University) ;
  • Park, Choon-Keun (Department of Animal Biotechnology, Kangwon National University) ;
  • Yang, Boo-Keun (Division of Animal Resource Science, Kangwon National University) ;
  • Cheong, Hee-Tae (School of Veterinary Medicine, Kangwon National University)
  • 최용락 (강원대학교 수의학부대학) ;
  • 이유미 (강원대학교 수의학부대학) ;
  • 김호정 (강원대학교 수의학부대학) ;
  • 박주희 (강원대학교 수의학부대학) ;
  • 권대진 (강원대학교 수의학부대학) ;
  • 박춘근 (강원대학교 동물생명공학과) ;
  • 양부근 (강원대학교 동물자원학부) ;
  • 정희태 (강원대학교 수의학부대학)
  • Published : 2008.03.31
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Abstract

We attempted to control the maturation promoting factors (MPF) activity and nuclear remodeling of somatic cell nuclear transfer (NT) bovine embryos. Bovine ear skin fibroblasts were fused to enucleated oocytes treated with either 5 mM caffeine for 2.5 h or 0.5 mM vanadate for 0.5 h and activated. The nuclear remodeling type of the reconstituted embryos was evaluated 1.5 h after activation. MPF activity was assessed in enucleated and chemical treated oocytes before the injection of a donor cell. Effect of chemicals on the embryonic development was evaluated with parthenogenetic embryos. MPF activity increased significantly by caffeine treatment, but decreased by vanadate treatment (p<0.05). Caffeine or vanadate had no deleterious effect on the parthenogenetic embryo development. In caffeine treated group, premature chromosome condensation (PCC) was occurred in 72.2% of NT embryos (p<0.05). In contrast, vanadate induced the formation of a pronucleus-like structure (PN) in a high frequency (68.9%, p<0.05) without PCC (NPCC). Blastocyst development of NT embryos increased by treating with caffeine (30.3%), whereas decreased by treating with vanadate (11.4%) compared to control (22.1%, p<0.05). The results indicate that caffeine or vanadate can control of MPF activity and remodeling type of NT embryos, resulting in the increased or decreased in vitro development.

Keywords

References

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