DOI QR코드

DOI QR Code

Delvo test, Eclipse 100 및 Parallux를 이용한 산양유의 잔류항균물질 검출 비교

Comparison of Antimicrobial Residue Detection in Goat Milk by the Delvo, Eclipse 100, and Parallux Tests

  • 발행 : 2008.03.30

초록

본 연구는 우유내 잔류물질 검사에 신속검출법으로 많이 사용되는 Delvo test가 산양유의 잔류물질 검사에도 적합한지 확인하기 위해 수행되었다. 8개 산양유목장 원유를 7회에 걸쳐 총 56개 시료를 Delvo test, Eclipse100, 그리고 Parallux로 분석하였다. 면역화학적 방법인 파라룩스에서는 양성 시료가 전혀 검출되지 않았으나 Delvo test에서는 37.5%의 양성율을 나타내었고 같은 미생물적 검출법인 Eclipse100에서는 3.6%의 양성율을 나타내었다. Delvo test는 국립수의과학검역원에서 발간한 원유검사표준화요령에 미생물학적 잔류물질 신속검출법으로 기재되어 있고, 축산물가공처리법상 "원유"의 정의가 '판매 또는 판매를 위한 처리 가공을 목적으로 하는 착유상태의 우유와 양유를 말한다'는 점을 고려할 때 Delvo test를 원유검사표준화요령에서 제외시키거나 Delvo test가 산양유의 잔류물질 신속검출법으로 적합하지 않다는 근거가 필요할 것으로 생각된다.

The aim of this work was to assess the Delvo test for the detection of antimicrobial residues in goat milk. A total of fifty six samples (eight farms, seven samplings each) were analyzed by the Delvo, Eclipse 100, and Parallux tests. None of the samples showed positive results with the Parallux test which is based on immune-chemical methods. However, 37.5% of samples showed positive results with the Delvo test. 3.6% of samples showed positive results with the Eclipse 100 test, which is based on a microbiological method. The Delvo test is included in the 'standard methods for the examination of raw milk' by the National Veterinary Research and Quarantine Service as a microbiological method used for the detection of antimicrobial residues. Because "raw milk" is defined as 'milked state of cow, ewe and goat milk for sale or for processing' in the Animal Food Products Processing Law, the Delvo test should be excluded from the 'standard method for the examination of raw milk', or additional official documents referring to the Delvo test as not appropriate for the detection of antimicrobial residues of goat milk are required.

키워드

참고문헌

  1. Althaus, R.L., Molina, P., Molina, A. Torres, A., and Fernandez,. N. (2002) $Detection^{\circledR}$ limits of antimicrobial agents in ewe milk by Delvotest. Milchwissenschaft. 57, 660-664
  2. Beukers, R. (1991) Some special aspects of $Delvotest^{\circledR}$. In: Inhibitory Substances in Milk-Current Analytical Practice. IDF Bull. No. 283. Ind Dairy Fed., Brussels, Belgium, pp. 20-23
  3. Brady, M. S. and Katz, S. E. (1988) Antibiotic/antimicrobial residues in milk. J. Food Prot. 51, 8-11 https://doi.org/10.4315/0362-028X-51.1.8
  4. Carlsson, A. and Bjrck, L. (1987) The effect of some indigenous antibacterial factors in milk on the growth of Bacillus stearothermophilus var. calidolactis. Milchwissenschaft. 42, 283-285
  5. Carlsson, A. and Bjrck, L. (1989) Lactoferrin and lysozyme in milk during acute mastitis and their inhibitory effect in Delvotest. J. Dairy Sci. 72, 3166-3174 https://doi.org/10.3168/jds.S0022-0302(89)79475-3
  6. Cullor, J. S. (1992) Test for identifying antibiotic residues in milk:how well do they work? Vet. Med. 87, 1235-1241
  7. Currie, D., Lynas, L., Kennedy, G., and Mc Caughey, J. (1998) Evaluation of modified EC four plate method to detect antimicrobial drugs. Food Addit Contam. 15(6), 651-660 https://doi.org/10.1080/02652039809374694
  8. Dewdney, J. M., Maes, L., Raynaud, J. P., Blanc, F., Scheid, J. P., Jackson, T., Lens, S., and Verschueren, C. (1991) Risk assessment of antibiotic residues of beta-lactams and macrolides in food-products with regard to their immunoallergic potential. Food Chem. Toxicol. 29, 477-483 https://doi.org/10.1016/0278-6915(91)90095-O
  9. Halbert, L. W., Erskine, R. J. Barlett, P. C., and Johnson, G. L. (1996) Incidence of false positives results for assays used to detect antibiotics in milk. J. Food Prot. 8, 886-888
  10. Ham, J. S., Noh, Y. B., Kim, S. I., Kim, H. S., Jeong, S. G., Chae, H. S., Ahn, J. N., Jo, Cheorun, and Lee, W. K. (2005) Changes of chemical, bacteriological, and allergenicity of raw milk by gamma irradiation. J. Korean Dairy Technol. Sci. 23, 93-98
  11. International Dairy Federation. (1991) Detection and confirmation of inhibitors in milk and milk products. IDF. Bull. No. 258. Int. Dairy Fed., Brussels, Belgium
  12. Macaulay, D. M. and Packard, V. S. (1981) Evaluation of methods used to detect antibiotic residues in milk. J. Food Prot. 44, 696-698 https://doi.org/10.4315/0362-028X-44.9.696
  13. Myr-Mkinen, A. (1990) T-101 test for antibiotic residues in milk. Scand. Dairy Inf. 2, 38-39
  14. Molina, M. P., Althaus, R. L., Balasch, S., Torres, A., Peris, C., and Fernandez, N. (2003a) Evaluation of screening test for detection of antimicrobial residues in ewe milk. J. Dairy Sci. 86, 1947-1952 https://doi.org/10.3168/jds.S0022-0302(03)73782-5
  15. Molina, M. P., Althaus, R. L., Torres, A., Peris, C., and Fernandez, N. (2003b) Antimicrobial agent detection in ewe milk by the microbial inhibitor test(Brilliant Black Reduction Test - BRT-Aim). Int. Dairy J. 13, 821-826 https://doi.org/10.1016/S0958-6946(03)00107-9
  16. Montero, A., Althaus, R. L., Molina, A., Berruga, I., and Molina, M. P. (2005) Detection of antimicrobial agents by a specific microbiological method (Eclipse100) for ewe milk. Small Ruminant Res. 57(2-3), 229-237 https://doi.org/10.1016/j.smallrumres.2004.07.006
  17. Mourot, D. and Loussourorn, S. (1981) Sensibilitdes ferments lactiques aux antibiotics utiliss en mdecine vtrinaire. Rec. Med. Vt. 157, 175-177
  18. Oliver, S. P., Duby, R. T., Prange, R. W., and Tritscler, J. P. (1984) Residues in colostrum following antibiotic dry cow therapy. J. Dairy Sci. 67, 3081-3083 https://doi.org/10.3168/jds.S0022-0302(84)81676-8
  19. Schiffmann, A. P., Schtz, M., and Wiesner, H. (1992) False negative and positive results in testing for inhibitory substances in milk. Factors influencing the brillant black reduction test (BRT). Milchwissenschaft. 47, 770-772
  20. Seymour, H., Jones, G. M., and Gilliard, M. L. (1988) Comparisions of on-farm screening test for detection or antibiotics residues. J. Dairy Sci. 71, 539-544 https://doi.org/10.3168/jds.S0022-0302(88)79587-9
  21. Tyler, J. W., Cullor, J. S., Erskine, R. J., Smith, W. L., Dellinger, J., and McClure, .K. (1992) Milk antimicrobial drug residue assay results in cattle with experimental, endotoxininduced mastitis. J. Am. Vet. Med. Assoc. 201, 1378-1381
  22. Van Eenennaam, A. L., Cullor, J. S., Perani, L., Cardner, T. A., Smith, W. L., Dellinger, J., Guterbock, W. M., and Jensen, L. (1993) Evaluation of milk antibiotic residue screening test in cattle with occurring clinical mastitis. J. Dairy Sci. 76, 3041- 3053 https://doi.org/10.3168/jds.S0022-0302(93)77644-4
  23. 농림부 국립수의과학검역원. 2001. 원유검사표준화요령. pp. 10-12

피인용 문헌

  1. Major Components of Caprine Milk and Its Significance for Human Nutrition vol.46, pp.2, 2014, https://doi.org/10.9721/KJFST.2014.46.2.121
  2. Effect of the presence of colostrum on microbial screening methods for antibiotic detection in goats’ milk vol.121, pp.2-3, 2014, https://doi.org/10.1016/j.smallrumres.2014.07.007
  3. Recent advances in exploiting goat's milk: Quality, safety and production aspects vol.89, pp.2-3, 2010, https://doi.org/10.1016/j.smallrumres.2009.12.033