Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

Effect of Soy Protein Hydrolyzate on Lipid Metabolism and Antioxidant Activity in the Rat

대두단백가수분해물이 흰쥐의 지질대사와 항산화에 미치는 영향

  • 한윤희 (용인대학교 식품영양학과) ;
  • 박상규 (건국대학교 생명분자정보화센터) ;
  • 김혜영 (용인대학교 식품영양학과)
  • Published : 2008.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the effect of soy protein hydrolyzate on lipid metabolism and antioxidant activity in the rat. Thirty-eight male rats of Sprague-Dawley strain were divided into five groups: casein, isolated soy protein (ISP), seoritae protein hydrolyzate (SH), soluble soy protein hydrolyzate (SS), and insoluble soy protein hydrolyzate (IS). The control diet (casein group) contained 20% casein protein and experimental diet contained 10% casein and 10% isolated soy-protein or soy-protein hydrolyzate. Fecal lipid content was increased and lipid apparent absorption rate was decreased significantly by the ISP group at the first week of experimental period. Blood triglyceride, total cholesterol, LDL-cholesterol and atherogenic index (AI) were decreased by soy protein hydrolyzate groups than casein group. Liver total lipid, triglyceride and cholesterol were not different among groups, but showed decreasing tendencies in soyprotein hydrolyzate groups. The lipid lowering effect was prominent in the IS group among soy protein hydrolyzate groups. Total antioxidant activity showed increasing tendency in the seoritae hydrolyzate group. Liver superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities also showed higher tendencies in the seoritae hydrolyzate group than other groups. In conclusion, insoluble soyprotein hydrolyzate was more effective in lowering body lipids and seoritae hydrolyzate had higher antioxidant capacity among soy protein hydrolyzates.

본 연구는 대두단백가수분해물의 섭취가 소화흡수율, 지질대사 및 항산화에 미치는 영향을 살펴보고자 흰쥐를 카제인, 분리대두단백 (ISP) 또는 대두단백가수분해물 (서리태단백분해물 (SH), 수용성단백분해물 (SS) 또는 불용성단백분해물 (IS)군으로 나누어 4주간 사육한 후 생리활성의 변화를 살펴보았으며, 그 결과를 요약하면 다음과 같다. 1) 지질소화흡수율은 실험 1주차에서 분리대두단백군 (ISP군)이 다른 군보다 유의적으로 낮았다. 불용성가수분해물군 (IS군)은 실험 4주차에서 다른 군들보다 콜레스테롤의 소화흡수율이 낮은 경향이었다. 2) 혈액 중성지방은 불용성가수분해물군 (IS군)이 카제인군보다 유의적으로 낮았고, 서리태가수분해물과 ISP군도 낮은 경향이었다. 총 콜레스테롤도 중성지방과 마찬가지로 불용성가수분해물군이 가장 낮았고 다른 대두가수분해물군들과ISP군도 카제인군보다 낮은 경향이었다. 대두가수분해물군들이 카제인군보다 HDL-cholesterol은 높이고, LDL-cholsterol은 낮추어서 동맥경화지수는 불용성, 수용성, 서리태가수분해물의 순으로 낮았다. 3) 총항산화력은 군 간에 유의적인 차이가 없었으나 서리태가수분해물군 (SH군)이 다른 군들에 비해 높은 경향이었다. 간의 항산화효소활성은 각 군 간에 유의적인 차이는 없었으나 서리태가수분해물군의 superoxide dismutase, glutathione peroxidase 및 catalase 활성이 카제인군과 다른 실험군들에 비해 높은 경향이었다. 결론적으로, 대두단백가수분해물군들은 카제인군보다 혈액 중성지질, 총콜레스테롤, LDL-cholesterol과 동백경화 지표 (AI)를 낮추어서 심혈관질환예방에 바람직한 것으로 나타났는데, 이러한 효과는 특히 불용성단백가수분해물군에서 뛰어났다. 한편, 항산화활성의 경우 총항산화력과 항산화효소활성이 서리태가수분해물군에서 높은 경향으로 나타나 앞으로 서리태의 항산화 효과의 원인물질을 규명하고 분리추출하는 등의 연구를 통해 우리나라의 전통 콩의 우수한 면모를 밝히는 연구가 더 필요하다고 사료된다.

Keywords

References

  1. Korea National Statistical Office, Annual report of mortality statistics, Seoul; 2005
  2. Ministry of Health and Welfare, Report on 2005 National Health and Nutrtion Survey, Seoul; 2006
  3. Nagata Y, Imaizumi K, Sugano M. Effects of soya-bean protein and casein on serum cholesterol levels in rabbits. Br J Nutr 1989; 4: 113-121
  4. Potter SM. Overview of proposed mechanism for the hypocholesterolemic effect of soy. J Nutr 1995; 125: 606S-611S
  5. Forsythe WA. Dietary protein, cholesterol and thyroxine; a proposed mechanism. J Nutr Sci Vitaminol 1992; 36: 595-593
  6. Choi YS, Lee SY. Cholesterol lowering effects of soybean products (curd or curd residue) in rats. J Korean Soc Food Sci Nutr 1993; 22: 673-677
  7. Kwon TW, Song YS. Health functions of soybean foods. Proceeding of IUFOST 96 regional symposium on non-nutritional health factors for future foods; 1996 Sep 13; Seoul; 1996
  8. Shin MK, Han SH. Effects of soybean extract on serum lipid contents in fed fat diet rats. Korean J Food Sci Technol 1999; 31 (3): 809-814
  9. Barnes S, Grubbs C, Setchell KDR, Carlson J. Soybeans inhibit mammary tumors in models of breast cancer. In: Pariza M, Liss A, editors. Mutagens and carcinogens in the diet. New York: Wiley Liss; 1990. p.239
  10. Messina MJ, Persky V, Setchell KDR, Barnes S. Soy intake and cancer risk, a review of in vitro and in vivo data. Nutr Cancer 1994; 21: 113 https://doi.org/10.1080/01635589409514310
  11. Wei H, Cai Q, Rahn RO. Inhibition of UV light and Fenton-induced oxidative DNA damage by the soybean isoflavone genistein. Carcinogenesis 1996; 17: 73-77 https://doi.org/10.1093/carcin/17.1.73
  12. Ritter MA, Morr CV, Thomas RL. In vitro digestibility of phytate- reduced and phenolics-reduced soy protein isolates. J Food Sci 1987; 52: 325-341 https://doi.org/10.1111/j.1365-2621.1987.tb06604.x
  13. Kim WJ. Soybean health and utilization. Seoul: Hyoilbooks; 2006
  14. Kim HYP, Han YH, Kim MH, Kim KS. Effects of different kinds of korean soybeans on body lipids and lipid peroxidation in rats. Korean J Nutr 2007; 40(3): 229-234
  15. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37: 911-917 https://doi.org/10.1139/y59-099
  16. Floher L, Becker R, Brigelius R, Lengfelder E, Otting F. Convenient assays for superoxide dismutase. In: Miquel J, Quintaniha AT, Weber H, editors. CRC handbook of free radicals and antioxidants in biomedicine; 1992. p.287-288
  17. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutae activity. J Lab Clin Med 1975; 35: 337-341
  18. Johansson LH, Hankan BLA. A spectrophotometric method of determination of catalase activity in small tissue samples. Anal Biochem 1988; 174: 331-336 https://doi.org/10.1016/0003-2697(88)90554-4
  19. Paglia DE, Valentine WM. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70(1): 158-169
  20. Lee HJ. Effects of dietary casein, soyprotein and mixed protein on body lipid components of rats. [masters thesis]. Seoul: Sungshin women's university; 1986
  21. Ko MK, Kwon TW, Song YS. Effects of yellow and black soybeans on plasma and hepatic lipid composition and fecal lipid excretion in rats. J Korean Soc Food Sci Nutr 1998; 27(1): 126-131
  22. Song YS, Kwon TW. Hypocholesterolemic effect of soybean and soy products. Food Industry Nutr 2000; 5(2): 36-41
  23. Chen JR, Chiou SF, Suetsuna K, Yang HY, Yang SC. Lipid metabolism in hypercholesterolemic rats affected by feeding cholesterol- free diets containing different amounts of non dialyzed soybean protein fraction. Nutrition 2003; 19: 676-680 https://doi.org/10.1016/S0899-9007(03)00072-8
  24. Potter SM, Baum JA, Teng H, Stillman RJ, Shay NF, Erdman JW. Soy protein and isoflavones: their effects on blood lipids and bone density in postmenopausal women. Am J Clin Nutr 1998;68: 375s-1379s
  25. Kim BN, Kim JD, Ham SS, Lee SY. Effects of spice added natto supplementation on the lipid metabolism in rats. J Korean Soc Food Sci Nutr 1995; 24: 121-126
  26. Terpstra AH, van Tintelen G, West CE. The hypocholesterolemic effects of dietary soy protein in rats. J Nutr 1982; 112: 810-817 https://doi.org/10.1093/jn/112.4.810
  27. Balmir F, Staack R, Jeffrey E, Jimenez MD, Wang L, Potter SM. An extract of soy flour influences serum cholesterol and thyroid hormones in rats and hamsters. J Nutr 1996; 126: 3046-3053 https://doi.org/10.1093/jn/126.12.3046
  28. Sirtori CR, Gatti E, Mantero O, Conti F, Agradi E, Tremoli E, Sirtori M, Fraterrigo L, Tavazzi L, Kritchevsky D. Clinical experiment with the soybean protein diet in the treatment of hypercholesterolemia. Am J Clin Nutr 1979; 32: 1645-1658 https://doi.org/10.1093/ajcn/32.8.1645
  29. Carroll KK, Kurowska EM. Soy consumption and cholesterol reduction: Review of animal and human studies. 1st International Symposium on The Role of Soy in Preventing &Treating Chronic Disease. 1994 Feb 20; Mesa, Arizona; 1994
  30. Pratt DE, Pietro C, Porter W, Giffee W. Phenolic antioxidants of soy protein hydrolyzates. J Food Sci 1981; 47: 24 https://doi.org/10.1111/j.1365-2621.1982.tb11018.x
  31. Kim MJ. Studies on functional compositions and physicochemical characteristics of korean traditional soybeans. [masters thesis]. Yongin: Yongin university; 2004
  32. Bae EA, Moon GS. A study on the antioxidative activities of Korean soybeans. J Korean Soc Food Sci Nutr 1997; 26(2): 203- 208