Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Gucheokbogol-tang in Proliferation and Calcification of Osteoblast

구척보골탕 추출물이 뼈모세포의 증식 및 석회화에 미치는 영향

  • Han, Deok-Hee (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Kang, Hee-Jeong (Department of Microbiology, High Technology, Dankook University) ;
  • An, Joung-Jo (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Jo, Hyun-Kyung (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Yoo, Ho-Rhyong (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Seol, In-Chan (Department of Internal Medicine, College of Oriental Medicine, Daejeon University) ;
  • Kim, Yoon-Sik (Department of Internal Medicine, College of Oriental Medicine, Daejeon University)
  • 한덕희 (대전대학교 부속한방병원 심계내과학교실) ;
  • 강희정 (단국대학교 첨단과학부 미생물학교실) ;
  • 안정조 (대전대학교 부속한방병원 심계내과학교실) ;
  • 조현경 (대전대학교 부속한방병원 심계내과학교실) ;
  • 유호룡 (대전대학교 부속한방병원 심계내과학교실) ;
  • 설인찬 (대전대학교 부속한방병원 심계내과학교실) ;
  • 김윤식 (대전대학교 부속한방병원 심계내과학교실)
  • Published : 2008.02.25
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Abstract

This study was purpose to investigate that Gucheokbogol-tang(GBT), mainly used in osteoporosis, has possible regulatory effect on bone regeneration by operating in proliferation and calcification of osteoblast. So that, their cytotoxicity and proliferation was investigated with MTT by ELISA, and morphological change was observed by optical microscope and electron microscope. And activation of alkaline phosphates(ALP) which is secreted in early stage of bone formation was measured, and accumulation of $Ca^{2+}$ in the process of calcification was investigated by alizaline red S assay(ARS). The results were as follows: The MG-63 cell, originated from human cell was activated in $10^{-5}$ treated group, and the level of ALP was also increased in the treated group, highest on the third day. And from the outcome of ARS assay of calcification process in the Gucheokbogol-tang(GBT) treated group for 21days. These results suggested that Gucheokbogol-tang(GBT) is effective in proliferation and calcification of osteoblast.

Keywords

References

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