The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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The Experimental Study on Anti-oxidant and Anti-inflammatory Effect Hyulbuchukeotanggamibang (HBCT)

혈부축어탕가미방(血府逐瘀湯加味方)의 염증(炎症) 병리(病理) 인자(因子)와 산화력(酸化力) 손상(損傷)에 미치는 영향

  • Han, Hye-Suk (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Lim, Hyun-Jung (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Shin, Sun-Mi (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Kim, Soo-Min (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Lee, Jung-Eun (Dept. of Oriental Medicine Graduate School, Daejeon University) ;
  • Yoo, Dong-Youl (Dept. of Oriental Medicine Graduate School, Daejeon University)
  • 한혜숙 (대전대학교 한의과대학 부인과교실) ;
  • 임현정 (대전대학교 한의과대학 부인과교실) ;
  • 신선미 (대전대학교 한의과대학 부인과교실) ;
  • 김수민 (대전대학교 한의과대학 부인과교실) ;
  • 이정은 (대전대학교 한의과대학 부인과교실) ;
  • 유동열 (대전대학교 한의과대학 부인과교실)
  • Published : 2008.02.29
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Abstract

Purpose: This study was performed to evaluate anti-inflammatory effects of Hyulbuchukeotanggamibang water extract (HBCT). Methods: In the study of anti-inflammatory effects, HBCT was investigated using cultured cells and a murine models. As for the parameters of inflammation, levels of several inflammatory cytokines and chemical mediators which are known to be related to inflammation were determined in mouse lung fibroblast cells (mLFCs) and RAW264.7 cells. Results: Prior to the experiment, we investigated the cytotoxicity of HBCT. HBCT showed a safety in cytotoxicity on mLFCs. In experiment of anti-inflammatory effect, HBCT effected scavenging activity on DPPH free radical, superoxide dismutase and superoxide anion radical. HBCT inhibited $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2 and NOS-II mRNA expression in a concentration-dependent manner in RAW264.7 cell line, and inhibited significantly $IL-1{\beta}$, IL-6, $TNF-{\alpha}$ production at $100{\mu}g/\;ml$ in a concentration-dependent manner. Conclusion: These results suggest that HBCT can be used for treating diverse female diseases caused by inflammation such as endometriosis, pelvic pain, cervicitis, pelvic inflammatory disease and pelvic tuberculosis and so forth.

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